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Preparation method for culturing ecological convenient bacteria

A convenient and ecological technology, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of chemical toxicity and residues, high price, and poor use, and achieves simple operation, low cost, and good effect. Effect

Inactive Publication Date: 2010-05-26
北京普仁生态技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most of the live bacteria products seen in the market are fermented in the factory after the bacteria agent and the product are fermented, and then packaged and distributed to the market. Defects such as instability and unreasonable cost performance
Most live bacteria products must be prepared by specialized reaction tanks and reactors in the factory production process. The technical requirements and economic investment are high, and some excipients and additives added in the preparation process are prone to chemical toxicity and residues. After being finalized and packaged, they are transported and sold to users. The links are many and the chains are long, which not only greatly reduces the activity of the product, but also tends to cause unstable use effects, and greatly increases the cost, making most microbial preparations more expensive. Often "unable to use" or "not easy to use", which affects the healthy development of the microbial industry

Method used

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  • Preparation method for culturing ecological convenient bacteria

Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment approach

[0006] The specific embodiment of product of the present invention is:

[0007] 1. Screening of strains: Select excellent strains, including lactic acid bacteria, Lactobacillus acidophilus, Lactobacillus plantarum, Bacillus subtilis, Streptococcus faecalis, Streptococcus faecium, Lactobacillus casei, Pediococcus lactis, Bifidobacterium , Bacillus natto, yeast, photosynthetic bacteria, Rhodopseudomonas palustris, and Bacillus licheniformis.

[0008] 2. Configure the culture medium: configure the culture medium with different components according to different types of bacteria. The main components of the culture medium are glucose, brown sugar, lactose, inorganic salt, starch, beef extract, yeast powder, yeast extract, peptone, agar, Water, Citric Acid, Lactic Acid, Acetic Acid, Sodium Hydroxide, Potassium Hydroxide, Phosphate Buffered Saline. The medium contains coordinating and growth-promoting factors, and its main components are vitamins, bases, porphyrin and its derivative...

Embodiment 1

[0014] Example 1: 10 g of commercially available freeze-dried photosynthetic bacterial strains; 15 g of yeast extract, 17.5 g of sodium acetate, 10 g of sodium chloride, 5 g of magnesium sulfate, 5 g of dipotassium hydrogen sulfate, and 10 g of citric acid were packaged separately. When using it, mix it, add 10,000ml of water and place it under the sun for about 7 days (about 35°C) to obtain a microbial preparation containing about 2 billion live bacteria per milliliter. The bacterial agent has a very good effect on enhancing the health status and growth promotion of farmed animals, especially aquatic animals, and can improve water quality.

[0015] The application effect of the product in the broiler farm:

[0016] 1. The average weight of broiler chickens (20 days old) in the test group was 750 grams per chicken, and the average weight of the control group was 670 grams per chicken;

[0017] 2. In this period (20 days), the total feed intake of the experimental group (1100 ...

Embodiment 2

[0019] Example 2: 5 g of commercially available freeze-dried lactic acid bacteria; 990 g of brown sugar and 10 g of yeast powder are packaged separately. When using it, mix it, add 9000ml of water and leave it at room temperature for about 2 days (about 20-25° C.), to obtain a microbial preparation containing about 1.5-2 billion viable bacteria per milliliter. The preparation has very good effects on preventing diarrhea and promoting growth of farmed animals.

[0020] Effect of the product on disease and mortality of piglets

[0021]

[0022] Compensatory effects of products on the stress of classical swine fever vaccine

[0023] group

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Abstract

The invention relates to a convenient preparation method for culturing ecological bacteria. The product comprises a composite microbial freeze-drying strain, a special culture medium, a flora coordination and growth promotion factor and the like. After the freeze-drying microbial strains and nutrients are mixed according to the weight ratio of 1:100-1:100000, finished products are obtained by sealing and encapsulating. When in use, the product is added with water with the quantity of 20 times, the mixture is stirred evenly, the environmental temperature is controlled between 15 DEG C and 40 DEG C, and the mixture is covered by a cover, sealed at a dry position and then can be fed after being naturally activated for 2-3 days. The added water is required to be drinking water and does not contain a disinfector, a bactericide, an antibiotic and a synthetic antibacterial agent, and the temperature of the water can not exceed 45 DEG C, warm water with the temperature of 25-37 DEG C is preferred; a used container must be washed and cleaned, is provided with a cover for sealing and can be recycled by disinfection after being used. At present, most of the active bacterium products sold in the market are packaged and delivered to the market after a microbial agent and the products are fermented in a factory, which is a sale-after-growth mode; the defects of unstable effect, unreasonable performance-price ratio and the like generally exist in the active bacterium products produced by this mode. The invention adopts a growth-after-sale mode and hands the production priority of a microbial preparation to a user, and the user can prepare and culture the required ecological bacteria under a simple condition by carrying out water addition, temperature control and natural fermentation on the product. The convenient bacteria do not contain chemical medicaments and toxin and are ecological and environment-friendly.

Description

technical field [0001] The invention relates to the field of preparation of microbial preparations, in particular to an ecological and convenient preparation method for cultivating microorganisms. technical background [0002] In recent years, microorganisms and their preparations have been more and more widely used in fields such as planting, breeding, environmental protection, medical treatment, and health care, and their preparation methods are even more varied. At present, most of the live bacteria products seen on the market are fermented in the factory after the bacteria agent and the product are fermented, and then packaged and distributed to the market. Defects such as instability and unreasonable cost performance. Most live bacteria products must be prepared by specialized reaction tanks and reactors in the factory production process. The technical requirements and economic investment are high, and some excipients and additives added in the preparation process are ...

Claims

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Application Information

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IPC IPC(8): C12N1/00C12R1/10C12R1/645C12R1/07C12R1/01C12R1/245C12R1/46C12R1/125C12R1/25C12R1/23C12R1/225
Inventor 秦泽荣曹学锋罗光建赵国栋
Owner 北京普仁生态技术有限公司
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