Method for applying gold nanoparticles mimetic enzyme in biological detection

Abstract

A method for applying gold nanoparticles mimetic enzyme in biological detection adopts gold nanoparticles instead of horse radish peroxidase (HRP) in the biological detection. The detection comprises the following steps: coupling the gold nanoparticles and a specific molecular probe to construct a specific nano-probe; performing specific binding between the nano-probe and the corresponding target molecule to be detected; developing with coloring solution containing peroxide and hydrogen-donating substrate, and measuring absorbance or performing microscopic observation so as to realize the qualitative and quantitative detection of the target molecule. The method belongs to the nanometer material and biomedical nanometer technical field. In the method, the size of the used gold nanoparticles mimetic enzyme is 1-1000nm, the gold nanoparticles mimetic enzyme can imitate HRP to catalyze peroxide and hydrogen-donating substrate to perform a color development reaction, and the enzymatic activity increases with the decrease of the size of the gold nanoparticles. The method of the invention uses the gold nanoparticles to label antibody and other biological molecules to build the similar enzyme labeled antibody and other diagnose preparations, thus having extensive application value.

Description

technical field [0001] The invention relates to the discovery of gold nanoparticle-simulating enzymes, and provides a method for using it to replace horseradish peroxidase for biological detection, belonging to the field of nanometer materials and biomedical nanotechnology. Background technique [0002] Gold nanoparticles are a typical representative of metal nanoparticles, which have a wide range of applications in biomedicine. Gold nanoparticles refer to gold materials with a diameter of gold particles between 1-100nm, and are one of the most stable heavy metal nanoparticles. Gold sols dispersed in water, namely colloidal gold, are often used in immunoassays. Gold nanoparticles are mesoscopic particles with special electronic structure and surface plasmon characteristics. Gold nanoparticles can be synthesized by common reagents such as chloroauric acid and sodium citrate or sodium borohydride, and the reaction process is simple, and the diameter of nanoparticles can be c...

AI Technical Summary

Problems solved by technology

[0006] Analyzing the application of the above gold nanoparticles in biological detection and sensing, the principle is based on the surface plasmon optical properties of gold nanoparticles (aggregate discoloration, Raman enhancement, etc.), microscopic imaging properties, adsorption and electron transfer properties, Au Ion spectroscopy and electrochemical detectable characteristics, as well as catalytic silver deposition and dyeing characteristics, have not yet involved the use of gold nanoparticles' simulated enzyme characteristics to catalyze the color development of substrates for detection.

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