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Reproduction method of Boston fern by tissue culture of sprout

A tissue culture and fern technology, applied in the field of propagation of Boston fern seedling tissue culture, can solve the problems of high cost, slow propagation speed, high production cost medium cost, etc., achieve low cost, fast propagation speed, simplify Effects of culture conditions or culture methods

Inactive Publication Date: 2010-05-12
珠海市园艺研究所 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Generally speaking, this process needs more than 6~8 months, and needs higher production cost (comprising culture medium cost and culture condition consumption) etc., so existing Boston fern seedling propagation technology still has the following defects: propagation speed slow, expensive

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] (1) Induction and differentiation steps of buds: get the root hairs of Boston fern as explants; the root hairs are first treated with 75% alcohol for 8 seconds, and then soaked in 0.1% mercuric chloride solution for 8 minutes Take it out, then rinse it 4 times with sterile water; get the root hairs sterilized by mercuric chloride solution and cut them into 1cm segments, put them into MS culture medium with 3% sugar water, without adding agar; The temperature is 24-26 degrees, the light is 1800lux, and the light time is 10 hours a day. After 6 weeks of cultivation, it can be seen that the two ends of the roots have differentiated into round bulbs.

[0013] (2) Proliferation and cultivation of sterile vaccines

[0014] After getting the spherical corm chopped, sprinkle it with 0.25mg / L of 6-benzylaminopurine (BA), sugar is 3%, in the MS medium of kinetin (KT) of 0.25mg / L, cultivate six After a week, each bulb can differentiate into 5 new bulbs; cultivation temperature re...

Embodiment 2

[0018] (1) Induction and differentiation steps of buds: get the root hairs of Boston fern as explants; the root hairs are first treated with 75% alcohol for 12 seconds, and then soaked in 0.1% mercuric chloride solution for 12 minutes Take it out, then rinse it with sterile water for 5 times; get the root fibers sterilized by mercuric chloride solution and cut them into 1cm segments, put them into MS culture medium with 3% sugar water, without adding agar; The temperature is 24-26 degrees, the light is 2500 lux, and the light time is 14 hours a day. After 6 weeks of cultivation, it can be seen that the two ends of the roots have differentiated into round bulbs.

[0019] (2) Proliferation and cultivation of sterile vaccines

[0020] After getting the round corm chopped, sprinkle it with 0.8mg / L of 6-benzylaminopurine (BA), sugar is 3%, in the MS medium of kinetin (KT) of 0.8mg / L, cultivate six After a week, each bulb can differentiate into 6 new bulbs; culture temperature requ...

Embodiment 3

[0024] (1) Induction and differentiation steps of buds: get the root hairs of Boston fern as explants; the root hairs are first treated with 75% alcohol for 10 seconds, and then soaked in 0.1% mercuric chloride solution for 10 minutes Take it out, then rinse 4 times with sterile water; get the root hairs sterilized by mercuric chloride solution and cut them into 1cm segments, put them into MS culture medium with 3% sugar water, without agar; culture temperature The temperature is 24-26 degrees, the light is 2000lux, and the light time is 12 hours a day. After 6 weeks of cultivation, it can be seen that the two ends of the roots have differentiated into round bulbs.

[0025] (2) Proliferation and cultivation of sterile vaccines

[0026] After getting the round corm chopped, sprinkle it with 0.5mg / L of 6-benzylaminopurine (BA), sugar is 3%, in the MS medium of kinetin (KT) of 0.5mg / L, cultivate six After a week, each bulb can differentiate into 5 new bulbs; cultivation temperat...

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PUM

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Abstract

The invention discloses a reproduction method of Boston fern by tissue culture of sprout and aims at providing a reproduction method of Boston fern by tissue culture of sprout with high reproduction speed and low cost. The reproduction method includes the steps of (a) induction and differentiation step of sprout; (b) propagation culture step of germ-free sprout; and (c) domestication transplanting step of germ-free sprout. The method puts the root hair of Boston fern in MS culture medium added with 3 percent of white sugar water without adding agar, thus being capable of effectively promoting the quick differentiation of the root hair of Boston fern to form round carmus. As the method adopts 0.25-0.8mg / L 6-benzyladenine (BA) 3 percent of sugar, and 0.25-0.8mg / L MS culture medium of kinetin (KT) with as propagation culture medium, the round carmus can propagate vigorously. Finally, the round carmus is put in the MS culture medium with 0.5-1.5mg / L 3- indolebutyric acid (IBA) and 3 percent of sugar, and then the round carmus can quickly root all around. The reproduction method can be widely applied to the field of tissue culture of sprout of Boston fern.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a propagation method for tissue culture of Boston fern seedlings. Background technique [0002] Boston fern, scientific name: Nephralepis exaltata cu. Bastaniensis, English name: Boston fern. Country of Origin: USA. Nephronaceae, plant dwarf, cluster type. Boston fern is a mutant species of Kidney-leaf fern, which later surpassed Kidney-leaf fern in fame. This fern is extremely adaptable and easy to grow, making it very popular. Regardless of garden landscaping, home greening or flower arrangement art, Boston fern is widely used, and it can be called the most living fern. In the previous method of propagating by ramets, a Boston fern can only reproduce 6 to 8 plants a year. Because of its low reproduction coefficient, it is far from meeting the needs of the market. Or adopt the spore propagation method, but because of collecting spore difficulty, cause spore impurity, the reason...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 金剑平文方德巫仕荣徐红黄皑冰姚慧芬
Owner 珠海市园艺研究所
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