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Hypoglycemic active part and hypoglycemic effective component of madder, preparation method and application thereof

A technology of effective parts and active ingredients, which is applied in the effective part of madder for lowering blood sugar and its preparation, can solve the problems of high cost and intestinal side effects, and achieve safe price, good social value and economic value, and a wide range of sources of diabetes treatment drugs Effect

Inactive Publication Date: 2010-03-10
HENAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing drugs of this type are costly and have intestinal side effects

Method used

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  • Hypoglycemic active part and hypoglycemic effective component of madder, preparation method and application thereof
  • Hypoglycemic active part and hypoglycemic effective component of madder, preparation method and application thereof
  • Hypoglycemic active part and hypoglycemic effective component of madder, preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] This example is the extraction method and preliminary activity screening of each extract of Rubia radiata, that is, the effective part.

[0023] The preparation method of the effective part of madder for lowering blood sugar is as follows: crush 500 g of madder rhizome, moisten with acetic acid, extract twice with chloroform and methanol respectively at room temperature, filter, recover the solvent, and obtain the effective part of chloroform for lowering blood sugar (Rc, 4.5g) and methanol hypoglycemic effective fraction (Rm, 12.8g).

[0024] Activity assay:

[0025] Method: Microplate method

[0026] Principle: α-D-glucosidase catalyzes the hydrolysis of 4-nitrobenzene-α-D-glucopyranoside (PNP-G) to produce nitrophenol (PNP, a yellow substance with maximum absorption at around 400m), α -Glycosidase inhibitors can inhibit the binding of α-glucosidase to the substrate to reduce the release of PNP. The enzyme inhibitory activity of the extract was calculated by the ch...

Embodiment 2

[0036] This example is a method for preparing the active ingredient, that is, the active compound, from the active part of Rubia chloroform under the guidance of activity.

[0037] The specific method is: the effective part of chloroform (4.5g) obtained in Example 1 is subjected to 200-300 mesh silica gel column chromatography, petroleum ether-acetone gradient elution (95:5-9:1), and 4 parts are obtained, of which Fraction 3 (Rc-3, 1.6 g) had α-glucosidase inhibitory activity (Table-2). This part was eluted by silica gel H column chromatography, petroleum ether-acetone (15:1), combined by TLC detection, and subjected to Sephadex LH-20 column chromatography, acetone eluted to obtain compound 1 (12.5mg), compound 2 (5.5mg ) and compound 3 (120.7 mg).

Embodiment 3

[0039] This example is the determination of the activity of the compounds in Rubia radiata inhibiting α-glucosidase.

[0040] Measuring method: with embodiment 1.

[0041] Determination results: The experimental results are shown in Table 2. The initial screening inhibition rates of the three compounds were all higher than the positive control Acarbose, while the IC 50 Values ​​are all lower than 50μg·mL -1 , far less than the clinically commonly used hypoglycemic drug Acarbose (IC 50 =1081.27μg·mL -1 ), has high α-glucosidase inhibitory activity, and can be developed as an active ingredient of madder for α-glucosidase inhibition.

[0042] The α-glucosidase inhibitory activity of active ingredient in table 2 madder

[0043]

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PUM

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Abstract

The invention relates to a hypoglycemic active part of madder and a preparation method thereof, an effective component obtained by extracting from the hypoglycemic active part and a preparation methodthereof, and application of the madder in reducing blood sugar and application of the active part and the effective component of the madder. The active part is a chloroform active part and a methanolactive part which are obtained by crushing and moistening the root of a medicinal plant, namely the madder, and orderly using chloroform and methanol for leaching. The invention adopts the Chinese medicament, namely the madder, as a raw material; the madder has rich sources, is a natural botanical medicament, and has the characteristics of relative safety and low cost; and the method provides a wider source of medicaments for treating diabetes, and has good social value and economic value. In addition, the method has important effect on the development of novel high-efficiency and safe medicaments for reducing the blood sugar after the meal.

Description

technical field [0001] The present invention relates to an effective part of madder for lowering blood sugar and a preparation method thereof, and also relates to an active ingredient extracted from the effective part for lowering blood sugar and a preparation method thereof, and also relates to the application of madder in reducing blood sugar and its effective part and its preparation method. Application of active ingredients. Background technique [0002] Diabetes mellitus is a systemic disease characterized by hyperglycemia, metabolic disorders, and closely related to insulin secretion. It is a clinical syndrome caused by the interaction of genetic and environmental factors. Absolute or relative insufficiency of insulin secretion and decreased sensitivity of target tissues to insulin lead to a series of metabolic disorders of sugar, protein, fat, water and electrolytes. Diabetes is mainly divided into two types: type I diabetes (ie, insulin-dependent diabetes) and type ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/74A61P3/10C07C50/18
Inventor 康文艺张丽宋艳丽
Owner HENAN UNIVERSITY
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