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Breeding method of dedicated silkworm hybrid secreting natural and green sericin with high content

A sericin, high-content technology, applied in animal husbandry and other directions, can solve the problems of not being used in production and perishing, and achieve the effect of promoting development and utilization, improving anti-oxidation, improving quality and product added value

Active Publication Date: 2010-03-10
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

Japan has preliminarily bred a silkworm variety "Sericin Star" with a sericin content of 98.5%, a cocoon layer volume of 83mg, and a white cocoon character. However, Japanese sericulture production has basically perished, and this variety has not been used in production; Chen Fusheng et al. (Anhui Agricultural Science, 2007, 35(32): 10356-10357; invention patent, publication number: CN101401563A) used the sericin silkworm germplasm resource Nd introduced from Japan to breed Japanese varieties with high sericin protein content Nd-s and the mid-line variety Nd-f were crossed to obtain a variety Nd-s×Nd-f that secretes high sericin content and has white cocoon traits

Method used

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  • Breeding method of dedicated silkworm hybrid secreting natural and green sericin with high content
  • Breeding method of dedicated silkworm hybrid secreting natural and green sericin with high content

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Embodiment 1

[0018] Embodiment 1: Breeding of sericin-specific silkworm hybrids

[0019] The naked pupa variety with Nd-s gene was selected from the existing silkworm germplasm resources in my country. Cocoons (naked pupae) or very thin sericin cocoons. The female silkworms of naked pupal varieties were used as female parents, and they were respectively crossed with male silkworms of dimorphic, Japanese, and high-yielding conventional varieties Baiyu, Xia 6, and 54A. The first to fourth generations were self-selected, and the offspring were all mixed. For ant mass breeding, according to the phenotype of the Nd-s gene (sericin cocoon, which can be easily spread by hand), each generation selects 30 female and male individuals with sericin cocoons and common markings, and conducts sericin cocoons one by one. Content tracking and detection, select female and male individuals with sericin content above 99.0% and cocoon layer content above 60mg for mating and offspring to accelerate the homozygo...

Embodiment 2

[0022] Embodiment 2: the main character of the silkworm kind of selection

[0023]On the basis of screening a large number of breeding materials and applying the designed variety breeding technical plan, three special silkworm original species with a sericin content of more than 99.0% have been bred, namely SC5, SC6 and SC8, which were bred in the spring of 2009. The amount of the cocoon layer is 71mg, 73mg, 70mg respectively, and the content of sericin is 100%. The above three Japanese original species were crossed with the conventional variety A3 with green cocoon traits, and compared with the top-crossing test, a pair of silkworm hybrids SC5×A3 special for secreting high-content green sericin were selected and bred. They were bred in the spring of 2009 and produced cocoons. The layer weight reaches 93 mg, and the sericin content is 99.88%. The main properties are shown in Table 1.

[0024] Table 1. Silkworm performance and sericin content of the original and hybrid varieti...

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Abstract

The invention provides a breeding method of dedicated silkworm hybrid secreting natural and green sericin with high content, comprising the following steps: taking exarate pupae breeds (with Nd-s genes) as stuff to be hybridized with the breeds with high amount of silk production, and breeding Japanese special original strains with sericin content being more than 99.0% and homozygous genotypes (Nd-s / Nd-s) by adopting the methods of selfing, backcrossing, marker gene selecting, sericin content tracking and detecting and the like; and hybridizing the Japanese special original strains with the Chinese conventional breeds (Gc / Gc) with green cocoon properties to breed the dedicated silkworm hybrid. The method can provide high quality raw materials to such fields as cosmetics, medicines, food, fiber modified materials and the like and improve the product added value.

Description

technical field [0001] The present invention relates to the breeding of silkworm varieties with special purposes. Specifically, it uses methods such as hybridization, self-crossing, backcrossing, marker gene selection, trace detection of sericin content, and determination of combining ability to select and breed silkworms that secrete pure natural, high-content, A method for special-purpose silkworm hybrids for green sericin. Background technique [0002] my country is the largest producer and supplier of cocoon and silk in the world. The output and export volume of cocoon and silk account for more than 70% and 80% of the world's total respectively. Silk is a natural animal protein, mainly composed of two proteins: fibroin in the inner layer and sericin in the outer layer. Silk fibroin is a high molecular weight insoluble fibrin, accounting for 70-80%. The current silk industry mainly uses silk fibroin to make raw silk and silk products; sericin is a soluble globular protei...

Claims

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Application Information

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IPC IPC(8): A01K67/04
Inventor 王永强孟智启胡桂燕曹锦如计东风陈诗姚陆松叶爱红何秀玲刘培刚
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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