Human aldehyde dehydrogenase 2 sequence designed according to preferred codon of pichia pastoris
A technology of acetaldehyde dehydrogenase and codon preference, applied in fermentation, botany equipment and methods, biochemical equipment and methods, etc., can solve the problem of narrow access to acetaldehyde dehydrogenase
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[0045] 1. Acquisition and verification of plasmid pPIC9K-ALDH2
[0046] 1. Target sequence design and synthesis:
[0047] The human acetaldehyde dehydrogenase 2 sequence is designed according to the preferred codon of Pichia pastoris, which is characterized in that: the DNA molecule is designed according to the preference of Pichia pastoris, and the molecule is encoded with the gene ID 217 of the human acetaldehyde dehydrogenase 2 gene. A sequence of nucleotides that is identical to a protein at the amino acid level.
[0048] The human acetaldehyde dehydrogenase 2 gene sequence designed according to the preferred codons of Pichia pastoris is based on the human ALDH2 gene (gene ID 217) [from the human ALDH2 DNA (gene ID217) sequence on Genebank], delete UTR and Signal peptide and endonuclease EcoR I and endonuclease Not I restriction sites, and then replace the codon GCG encoding Ala with GCU according to the codon usage of Pichia pastoris, and replace the codon CGC and CGG en...
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