Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Human aldehyde dehydrogenase 2 sequence designed according to preferred codon of pichia pastoris

A technology of acetaldehyde dehydrogenase and codon preference, applied in fermentation, botany equipment and methods, biochemical equipment and methods, etc., can solve the problem of narrow access to acetaldehyde dehydrogenase

Inactive Publication Date: 2010-02-17
WUHAN INSTITUTE OF TECHNOLOGY
View PDF1 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, there have been some reports of anti-alcoholic drugs containing aldehyde dehydrogenase, but the narrow access to aldehyde dehydrogenase has become the bottleneck of this type of health care products

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Human aldehyde dehydrogenase 2 sequence designed according to preferred codon of pichia pastoris
  • Human aldehyde dehydrogenase 2 sequence designed according to preferred codon of pichia pastoris
  • Human aldehyde dehydrogenase 2 sequence designed according to preferred codon of pichia pastoris

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0045] 1. Acquisition and verification of plasmid pPIC9K-ALDH2

[0046] 1. Target sequence design and synthesis:

[0047] The human acetaldehyde dehydrogenase 2 sequence is designed according to the preferred codon of Pichia pastoris, which is characterized in that: the DNA molecule is designed according to the preference of Pichia pastoris, and the molecule is encoded with the gene ID 217 of the human acetaldehyde dehydrogenase 2 gene. A sequence of nucleotides that is identical to a protein at the amino acid level.

[0048] The human acetaldehyde dehydrogenase 2 gene sequence designed according to the preferred codons of Pichia pastoris is based on the human ALDH2 gene (gene ID 217) [from the human ALDH2 DNA (gene ID217) sequence on Genebank], delete UTR and Signal peptide and endonuclease EcoR I and endonuclease Not I restriction sites, and then replace the codon GCG encoding Ala with GCU according to the codon usage of Pichia pastoris, and replace the codon CGC and CGG en...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Upstream primeraaaaaaaaaa
Login to View More

Abstract

The invention relates to a gene sequence, in particular to a human aldehyde dehydrogenase 2 sequence designed according to a preferred codon of pichia pastoris. The sequence is characterized in that aDNA molecule is designed according to the preference of the pichia pastoris, and a code of the molecule has the same nucleotide sequence as a protein coded by a human aldehyde dehydrogenase 2 gene ID217 on an amino acid level. The human aldehyde dehydrogenase 2 gene sequence designed according to the preferred codon of pichia pastoris is realized by the following steps: on the basis of a human ALDH2 gene (gene ID 217), UTR and signal peptide are deleted; enzyme cutting sites of an incision enzyme EcoR I and an incision enzyme Not I are added; a codon GCG for coding Ala is replaced by GCU according to the use method of the pichia pastoris codon, and codons CGC and CGG for coding Arg are replaced by AGA, AGG, CGU and CGA; and altogether 28 bases are modified to obtain a target sequence ALDH2. The gene sequence can express with high efficiency in the pichia pastoris, can be applied to preventing alcoholism and protecting livers and has the effects of preventing alcoholism and protecting livers.

Description

technical field [0001] The present invention relates to a gene sequence. Background technique [0002] With the rapid development of the economy and the continuous improvement of people's living standards, people pay more and more attention to high-quality life, the most important of which is health. However, economic development is accompanied by changes in people's diet structure, increased work and life pressure, reduced exercise, and deterioration of the living environment and other factors that are unfavorable to health. The incidence of various diseases, especially malignant diseases, is increasing year by year. Among them, liver cancer is one of the common malignant tumors in my country, with a high mortality rate. It ranks third in the order of malignant tumor deaths after stomach and esophagus, and ranks second in rural areas in some areas. Second only to gastric cancer, about 110,000 people die from liver cancer every year in my country, accounting for 45% of the wo...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N15/53C12R1/84
Inventor 赵玉凤吴元欣雷明科邓凡池汝安黄锟赵锦
Owner WUHAN INSTITUTE OF TECHNOLOGY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products