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Application of recombinant pichia pastoris bacterial strain

A technology of Pichia pastoris and yeast, which can be applied to medical preparations without active ingredients, medical preparations containing active ingredients, and drug combinations, etc., and can solve the problems of narrow access to acetaldehyde dehydrogenase and the like

Active Publication Date: 2011-05-04
武汉工大科技园发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, there have been some reports of anti-alcoholic drugs containing aldehyde dehydrogenase, but the narrow access to aldehyde dehydrogenase has become the bottleneck of this type of health care products

Method used

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  • Application of recombinant pichia pastoris bacterial strain
  • Application of recombinant pichia pastoris bacterial strain
  • Application of recombinant pichia pastoris bacterial strain

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0021] 1. Acquisition and verification of plasmid pPIC9K-ALDH2

[0022] 1. Target sequence design and synthesis:

[0023] From the human ALDH2DNA (gene ID217) sequence on Genebank, delete the UTR and signal peptide, add endonuclease EcoR I and endonuclease Not I restriction sites, and then modify 28 bases according to the codon usage of Pichia pastoris The target sequence is ALDH2; the specific sequence is as follows:

[0024] CGGAATTCTCAGCCGCCGCCACCCAGGCCGTGCCTGCCCCCAACCAGCAGCCCGAGGTCT 60

[0025] TCTGCAACCAGATTTTCATAAACAATGAATGGCACGATGCCGTCAGCAGAAAAAACATTCC 120

[0026] CCACCGTCAATCCGTCCACTGGAGAGGTCATCTGTCAGGTAGCTGAAGGGGACAAGGAAG 180

[0027] ATGTGGACAAGGCAGTGAAGGCCGCCAGAGCCGCCTTCCAGCTGGGCTCACCTTGGAGAC 240

[0028] GTATGGACGCATCACACAGAGGCCGACTGCTGAACAGACTGGCCGATCTGATCGAGAGGG 300

[0029] ACAGAACCTACCTGGCTGCCTTGGAGACCCTGGACAATGGCAAGCCCTATGTCATCTCCT 360

[0030] ACCTGGTGGATTTGGACATGGTCCTCAAATGTCTCAGATATTATGCCGGCTGGGCTGATA 420

[0031] AGTACCACGGGAAAACCATCCCCATTGACGGAGAC...

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Abstract

The invention relates to application of a recombinant microorganism in genetic engineering, in particular to application of a recombinant pichia pastoris bacterial strain. The invention is characterized by being a pichia pastoris GS115-07ALDH2 strain which is applied to preventing alcoholism and protecting livers. The invention has the characteristic of good effects of preventing alcoholism and protecting livers.

Description

technical field [0001] The invention relates to the application of a genetically engineered recombinant microorganism. Background technique [0002] With the rapid development of the economy and the continuous improvement of people's living standards, people pay more and more attention to high-quality life, the most important of which is health. However, economic development is accompanied by changes in people's diet structure, increased work and life pressure, reduced exercise, and deterioration of the living environment and other factors that are unfavorable to health. The incidence of various diseases, especially malignant diseases, is increasing year by year. Among them, liver cancer is one of the common malignant tumors in my country, with a high mortality rate. It ranks third in the order of malignant tumor deaths after stomach and esophagus, and ranks second in rural areas in some areas. Second only to gastric cancer, about 110,000 people die from liver cancer every ye...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N11/10A61K38/44A61K47/36A61P1/16C12R1/84
Inventor 吴元欣赵玉凤雷明科邓凡黄锟赵锦王红波王存文
Owner 武汉工大科技园发展有限公司
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