Production process for extracting and preparing high-purity 5-hydroxytryptophan from Griffonia simplicifolia

A technology for pentahydroxytryptophan and production process, which is applied in the field of production technology for preparing high-purity pentahydroxytryptophan, can solve the problems of slow extraction speed, low yield, low purity and the like, achieves good quality and improved product yield , the effect of high product yield

Inactive Publication Date: 2010-02-17
三明华健生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It solves the problems of low yield, low purity, variegated colors caused by other pigments, affecting product quality, and slow extraction speed in the extraction of five hydroxyls and tryptophan from plants in the pri

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Grizzly seeds were crushed to 30 mesh with CO 2 The supercritical extraction method removes oil once, the temperature is controlled at 45°C, the extraction time is 1 hour / time, the extraction pressure is controlled at 30mPa, CO 2 The flow rate is controlled at 20L / h.

[0022] The material after degreasing is extracted 3 times with 10 times of weight methanol, and the extraction temperature is controlled at 30 DEG C, and each extraction time is 1 hour; Merge extract, microfiltration (microfiltration adopts polymer microfiltration membrane, and pressure is controlled at 2kPa, After ultrafiltration (using ultrafiltration membrane, the membrane pore size is 200A°), it is adsorbed by ion exchange resin, eluted with NaOH aqueous solution with a pH value of 9, concentrated in vacuo, crystallized at 4°C, crystallized for 5 hours, and centrifuged. Microwave drying was carried out for 15 minutes, and the temperature was controlled at 50°C; 98.2% dry powder of pentahydroxytryptop...

Embodiment 2

[0024] Crush Ghana seeds to 20 mesh, using supercritical CO 2 The extraction method extracts and removes oil once, the temperature is controlled at 0-10°C, the extraction time is 1 hour / time, the extraction pressure is controlled at 20mPa, CO 2 The flow rate is controlled at 10L / h. The material after degreasing is extracted 5 times with 15 times of weight ethanol again, and the extraction temperature is controlled at 60°C, and the extraction time is 1 hour each time; After ultrafiltration (using ultrafiltration membrane, the membrane pore size is 500A°), it is adsorbed by ion exchange resin, eluted with KOH solution with a pH value of 8, concentrated in vacuo, crystallized at -20°C, crystallized twice, each crystallization The time is 10 hours, centrifugal separation, microwave drying for 60 minutes, and the temperature is controlled at 30° C. to obtain 98.3% pentahydroxytryptophan dry powder, with a yield of 90.2%.

Embodiment 3

[0026] Grizzly seeds are crushed to 100 mesh, using supercritical CO 2 The extraction method extracts and removes oil once, the temperature is controlled at 40-50°C, the extraction time is 1 hour / time, the extraction pressure is controlled at 25mPa, CO 2 The flow rate is controlled at 30L / h. The material after degreasing is extracted 4 times with 10 times of quality 60wt% methanol again, and the extraction temperature is controlled at 40 ℃, and each time of extraction is 2 hours; ), ultrafiltration (Ultrafiltration membrane, membrane pore size at 300A°), adsorption by ion exchange resin (resin type), elution with KOH solution with a pH value of 10, vacuum concentration, crystallization at 10-0°C, The crystallization time was 12 hours, centrifuged, microwave dried for 30 minutes, and the temperature was controlled at 35° C. to obtain 98.3% pentahydroxytryptophan dry powder, with a yield of 91.2%.

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Abstract

The invention provides a production process for extracting and preparing high-purity 5-hydroxytryptophan from Griffonia simplicifolia, belonging to the technical content of extracting 5-hydroxytryptophan from plants and solving the problems of low yield, low purity and influence on product quality, low extraction speed and the like due to variegation generated by other pigment in the process of extracting 5-hydroxytryptophan from plants in the prior art. The production process comprises the following steps: using Griffonia simplicifolia produced in Africa as raw material, carrying out supercritical extraction for degreasing, extracting the degreased material for 3-5 times by using water or alcoholic solvents or mixed solution of water and alcoholic solvents; merging the extraction solution, carrying out microfiltration, ultrafiltration, adsorption by using ion exchange resin, elution, vacuum concentration, lower-temperature crystallization, centrifugal separation and microwave drying to obtain high-purity 5-hydroxytryptophan dry powders. The process has the advantages of rapidness, high efficiency, high yield, good quality and the like, and is suitable for large-scale industrial production; and the purity of the obtained product can reach more than 99.5 percent, and the yield is more than 90 percent.

Description

technical field [0001] The invention belongs to the technology of extracting five-hydroxytryptophan from plants, in particular to a production process for extracting and preparing high-purity five-hydroxytryptophan from Ghana seeds. Background technique [0002] Ghana seeds are the dry seeds of Griffonia simplicifolia, a leguminous shrub plant. The seeds are oblong, about 1.5 cm long, and about 1 cm wide. Weighs about 80 grams. Wild in West African countries such as Ghana, Ivory Coast and Togo. The English name of 5-hydroxytryptophan is 5hydroxytryptophan, which is a natural amino acid extracted from Ghana seeds in Africa. Since the synthetic five-hydroxytryptophan and tryptophan are easily polluted during the fermentation process, compared with the synthetic five-hydroxytryptophan It is safer to use. [0003] The molecular formula of 5-hydroxytryptophan is C 11 h 12 N 2 o 3 , molecular weight 220.23, high-purity product is white fine powder, is a pure natural herbal ...

Claims

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Application Information

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IPC IPC(8): C07D209/20
CPCY02P20/54
Inventor 赵敏余峰陈燕梅
Owner 三明华健生物工程有限公司
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