On-site rapid high-sensitivity detection kit and detection method of taura syndrome virus (TSV)

Abstract

The invention relates to an on-site rapid high-sensitivity detection kit and a detection method of taura syndrome virus (TSV). The on-site rapid high-sensitivity detection kit comprises a sampling tube, a rinsing tube filled with distilled water, a nucleic acid denaturation tube filled with TE buffer liquid, an amplification detection tube filled with amplification reaction liquid and nucleic acid dye, a negative control tube, a positive control tube, a FTA membrane, quick drying liquid, and the like. The detection method has higher specificity, sensitivity and convenience than the TR-PCR detection method and has the advantages of low cost, no toxicity, high safety, convenient use, more accurate and quicker detection and high sensitivity and can replace the prior related detection methods, such as a pathological section method, an electron-microscopic observation method, an antibody detection method and a TR-PCR detection method. The on-site rapid high-sensitivity detection kit and the detection method can be both used in an indoor laboratory and a production site in the field, have significance for strengthening the TSV epidemiological monitoring and the disease control and prevention and have high popularization and application value.

Description

technical field [0001] The invention relates to a marine biological pathogen detection technology, in particular to an on-site rapid and high-sensitivity detection kit for prawn Taura syndrome virus (TSV for short) and a detection method thereof. Background technique [0002] Shrimp taura virus is a single-stranded RNA virus belonging to the Dicistroviridae family. The virus mainly infects Penaeus vannamei and Penaeus stylirostru, and can also infect Penaeus chinensis and Penaeus japonicus. (Penaeus japonicus), Penaeus monodon (Penaeus monodon), etc. Among them, Litopenaeus vannamei is the most sensitive, and the mortality rate of prawns after infection with the virus is as high as 40-95%. The virus once caused huge losses in the American shrimp farming industry. In recent years, with the rapid expansion of the culture scale of Litopenaeus vannamei in my country, the risk of large-scale occurrence of TSV has gradually increased. During the national epidemiological investiga...

AI Technical Summary

Problems solved by technology

The pathological section method cannot directly detect the virus, but can only detect the histopathological signs of the disease, and it is also limited to the laboratory; although the electron microscope technology can directly observe the existence of virus particles, its operation is complicated and costly. The time is long and the accuracy is low; although the detection of TSV by antibody detection method is faster than pathological section method, its sensitivity is low, and it is difficult to detect by antibody method before TSV has caused infection or the very early stage of infection; the RT of TSV -PCR detection method, although it overcomes the shortcomings of the previous methods, it can achieve relatively fast and accurate detection of TSV under laboratory conditions, but because conventional RT-PCR detection requires expensive PCR machines, gel electrophoresis and imaging system, making it difficult for RT-PCR detection method to be used for rapid detection on the spot, which greatly limits the application of RT-PCR detection method in production