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Method for quickly evaluating disease resistance of banana fusarium wilt

A technology for Fusarium wilt and Fusarium wilt of banana, applied in the field of agriculture-plant protection, to achieve the effect of repeatability and reliability assurance

Inactive Publication Date: 2012-07-18
POMOLOGY RES INST GUANGDONG ACADEMY OF AGRI SCI
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Problems solved by technology

Other resistance identification methods with greater improvement have not been reported so far

Method used

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  • Method for quickly evaluating disease resistance of banana fusarium wilt

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Embodiment Construction

[0016] Six varieties including Brazil, GCTCV-119, Formosana, Nongke 1, Guangfen 1 and Dongguan Dajiao were used as experimental materials. Inoculate the adventitious buds produced by the shoot tip explants of the above varieties into the rooting medium: 1 / 2MS+1.0mg / l NAA+0.2mg / l IBA+30g / l sucrose+2g / l activated carbon+6g / l agar. After rooting and culturing for 2-3 weeks, the rooting test-tube plantlets whose leaves have begun to unfold are transferred to MIS for pre-cultivation, and a 150ml triangular flask is used as a culture container, and one test-tube plantlet is inoculated in each bottle. After the rooted test-tube plantlets of the above varieties were grown in MIS medium for 1-2 weeks, select small plants with a plant height of 4.5-5 cm, the number of expanded leaves on the upper part of the plant ≥ 2, and the number of roots ≥ 3 to inoculate with Foc. When inoculating, open the sealing film of the triangular flask on the ultra-clean workbench, and use tweezers to pre-...

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Abstract

The invention relates to a method of quickly evaluating disease resistance of banana fusarium wilt, belonging to the technical field of plant protection of agriculture. The invention has the following steps: transferring test-tube plantlets which grow for 2-3 weeks in a root culture medium to an improved culture medium MIS to pre-culture for 1-2 weeks; selecting plantlets with the height of 4.5-5cm, the number of blades unfolded on the upper part of plant being more than or equal to 2 and the number of roots being more than or equal to 3 to inoculate Fusarium oxysporum f. sp. cubense (Foc) ona super-clean worktable in the phase of root culture of fast in vitro propagation of banana; placing a culture vessel in a tissue culture room with the temperature of 25 plus or minus 2 DEG C to observe the condition of pathogenesis; evaluating disease grades of singleplant rooted test-tube plantlet according to the disease evaluation grade from grade 0 to grade 6 24 days after inoculating FOC. Compared with the present widely adopted pot culture systems and water culture systems, the evaluation method provided in the invention is not only able to be closely linked with the research of breeding for disease resistance of banana, but also has the characteristics of accuracy and fast speed.

Description

technical field [0001] The invention relates to a method for quickly identifying the disease resistance of Fusarium wilt of banana, especially under the condition of in vitro culture, by improving the medium formula and inoculating the pathogenic bacteria in the rooting culture stage, quickly and effectively Screen banana disease-resistant germplasm resources and breeding materials to improve the efficiency of banana fusarium wilt-resistant breeding. The invention belongs to the technical field of agriculture-plant protection. Background technique [0002] Banana fusarium wilt (also known as Panama disease, yellow leaf disease) is one of the main diseases that threaten banana production in my country and even the world in recent years. Widely regarded as one of the most devastating plant diseases of all time, the disease is caused by the soil fungus Fusarium oxysporum f. sp. cubeense (E.F. Smith) Snyd. & Hans (Foc). Four physiological races of Foc have been confirmed, amon...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/18C12R1/77
Inventor 吴元立易干军彭新湘
Owner POMOLOGY RES INST GUANGDONG ACADEMY OF AGRI SCI
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