Strand substitute polymerase chain reaction
A technology of strand displacement and polymerase, applied in the field of molecular biology, can solve problems such as cross-contamination and difficult precise quantification
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[0083] Example 1 Multiple amplification of replacement reporter gene of HFMD enterovirus:
[0084] At present, the source of infectious diseases, including enteroviruses, is generally diagnosed by isolation and culture methods, gene cloning and sequencing methods, immunoserological and other diagnostic methods, and real-time fluorescent PCR methods for molecular detection. The detection results of the first two methods are the most reliable, but it takes a long time and depends on certain experimental technical conditions. The latter two are clinical diagnostic methods widely used in clinical testing.
[0085] Human enteroviruses are pathogenic strains of Picornaviridae, including enterovirus (EV) A, B, Coxsack virus (COX) A16, echovirus (echorirus30) and EVA new serotype EV71, EV71 includes BrCr, B1, 2, 3, 4, 5 and C1, 2, 3, 4 subtypes. The epidemic is sporadic in Asia all the year round, peaking in June and July, and the virus is prone to highly mutated, small epidemics in...
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