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FN test paper for quickly diagnosing bladder cancer and application thereof

A technology for rapid diagnosis and bladder cancer, applied in measurement devices, instruments, scientific instruments, etc., can solve problems such as unreported, and achieve the effect of easy to grasp, high sensitivity, intuitive and reliable result judgment

Inactive Publication Date: 2010-01-27
RUIJIN HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But, there is no report about a kind of FN test paper for rapid diagnosis of bladder cancer and its application

Method used

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  • FN test paper for quickly diagnosing bladder cancer and application thereof
  • FN test paper for quickly diagnosing bladder cancer and application thereof
  • FN test paper for quickly diagnosing bladder cancer and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Preparation of gold-labeled antibody complexes

[0029] 1. Colloidal gold preparation

[0030] Add 100ml of 0.005%-0.02% chloroauric acid solution into a round bottom flask and heat to boiling. After boiling, add freshly prepared 0.4%-2% trisodium citrate (Na 3 C 6 h 5 o 7 2H 2 O) 1-2.2ml of aqueous solution, after boiling for 10-25 minutes, continue to stir and cool to room temperature. During this process, it can be seen that the color change of the solution is: golden yellow→black→purple→dark blue→bright red. When the color of the solution completely changes to transparent bright red, the required colloidal gold is obtained. After cooling, put it into a dialysis bag and dialyze it against ultrapure water (1:5000) three times, and finally transfer the dialyzed colloidal gold to a clean glass bottle with a screw cap, and store it at 4°C in a dark environment. (Colloidal gold: HAuCl 4 Sinopharm Chemical Reagent Co., Ltd., trisodium citrate (Shanghai Qingxi Chemic...

Embodiment 2

[0039] Preparation of FN test paper for rapid diagnosis of bladder cancer

[0040] Please refer to figure 1 .

[0041] figure 1 It is a FN test paper for rapidly diagnosing bladder cancer. The FN test paper is provided with a bottom plate 1, and the bottom plate 1 is covered with a sample pad 2, a gold rubber pad 3, a nitrocellulose film layer 4, and a water-absorbing paper layer 5 in sequence. The base plate 1 is a PVC base plate, and the material of the sample pad 2 is urine-absorbing glass fiber. Preparation of gold gel pad 3: Spray the gold-labeled antibody solution prepared in Example 1 on a glass fiber membrane with a width of 1 cm, with a sample volume of about 2 ul / cm, and dry at 37°C. Production of nitrocellulose membrane layer 4: Spray the secondary antibody on the nitrocellulose membrane (NC membrane) as the quality control line (C line), and spray the anti-FN polyclonal antibody at a distance of 1 cm from the quality control line as the test line (T-line). The...

Embodiment 3

[0043] Detection of FN test paper for rapid diagnosis of bladder cancer

[0044] 1. Test paper detection method:

[0045] 1. Take 50ul of the sample to be tested and add it to the sample area of ​​the test strip;

[0046] 2. Observe and record the test results after 10 minutes. The results observed after 20 minutes are invalid.

[0047] 2. Judgment of test results:

[0048] 1. Positive result: a purple-red band appears on the test line and quality control line of the test strip. (See Figure 2A )

[0049] 2. Negative result: only a purple-red band appears on the quality control line of the test strip. (See Figure 2B )

[0050] 3. Invalid result: There is no purple-red band on the quality control line of the test strip. (See Figure 2C )

[0051] 3. Detection principle

[0052] See image 3 A few drops of urine sample are dropped on the sample pad. Due to the action of chromatography, the liquid flows toward the absorbent paper layer. When it flows through the gol...

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Abstract

The invention relates to an FN test paper for quickly diagnosing bladder cancer, which comprises a substrate, a sample pad, a gold colloidal pad, a nitrocellulose membrane layer and an absorbent paper layer, wherein the substrate is sequentially covered by the sample pad, the gold colloidal pad, the nitrocellulose membrane layer and the absorbent paper layer, and the gold colloidal pad comprises a gold-labelled antibody compound combining colloidal gold and anti-urine fibronectin antibodies. The invention also discloses application of the FN test paper for quickly diagnosing the bladder cancer. The FN test paper for quickly diagnosing the bladder cancer has the advantages of high speed, high sensitivity, strong specificity, good stability, simple and convenient operation, no need of any instrument or equipment, visual and reliable result judgment, easy grasp and suitability for diagnosis of the disease of bladder cancer and self-monitoring of the condition of the disease by detection of the urine fibronectin in urine.

Description

technical field [0001] The invention relates to a test paper, in particular to a FN test paper for rapidly diagnosing bladder cancer and its application. Background technique [0002] At present, the diagnosis of bladder cancer, especially the monitoring of recurrence after treatment, is mainly based on urine exfoliated cytology and cystoscopic biopsy, but urine exfoliated cytology is affected by many factors, and the positive rate is low; although cystoscopy is the most reliable Diagnostic method, but it is an invasive operation, has certain trauma and complications, and is not easy to be accepted and adhered to by patients. Urinary fibronectin (FN) is a widely studied new tumor marker for bladder cancer. Studies have shown that urinary FN is used as a diagnostic index for invasive bladder cancer, the diagnostic sensitivity is 76.47% to 100%, the specificity is 87.22%, and the diagnostic accuracy is 86.96%. At present, the clinical detection of urinary FN mainly adopts EL...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574G01N33/558
Inventor 沈周俊张敏光沈鹤柏周海清
Owner RUIJIN HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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