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Extraction process of paeoniflorin

An extraction process, the technology of paeoniflorin, applied in the field of paeoniflorin extraction process, can solve the problems of low productivity, poor product purity, unfavorable promotion of production, etc., and achieve the effect of high output rate, less processing equipment, and easy automatic control

Inactive Publication Date: 2010-01-20
张守力
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] An object of the present invention is to provide a simple and effective paeoniflorin extraction process with high product purity that can realize large-scale and industrialized production in order to solve the problems of the above-mentioned prior art with low productivity, poor product purity, and unfavorable promotion of production.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Weigh 1kg paeonol as the raw material, cut the raw material into about 1mm slices, and add 1kg solution prepared by 24g cellulase, the enzymatic hydrolysis temperature is 45°C, and the enzymatic hydrolysis time is 4 hours; after enzymatic hydrolysis, add 3 times the amount of water for decoction Boil 4 times, 1 hour each time, after boiling, let it stand and let it cool;

[0026] Centrifugal filtration: put the extract into a centrifuge, separate at 3000 rpm for 25 minutes, collect the supernatant, filter the centrifugate, and collect the filtrate for later use;

[0027] Elution: Concentrate the filtrate, put it on the D201 macroporous adsorption resin column, first elute the impurities with water, and then elute with 80% ethanol, the dropping speed is 2.0ml / (min·cm 2 ), collect the ethanol eluate, and the collected amount is 3 times of column volume;

[0028] Refining: Concentrate the eluent under reduced pressure to half, add activated carbon to decolorize and filter...

Embodiment 2

[0030] Weigh 10kg paeonol as the raw material, make the raw material into 40 mesh grains, and add 10kg solution prepared by 0.30kg cellulase and purified water, the enzymolysis temperature is 40°C, and the enzymolysis time is 3 hours; after enzymolysis, add Decoct 8 times the amount of water for 3 times, 1 hour each time, after decocting, let it stand and let it cool;

[0031] Centrifugal filtration: Put the extract into a centrifuge, separate it for 20 minutes at 2000 rpm, collect the supernatant, filter the centrifugate, and collect the filtrate for later use;

[0032] Elution: Concentrate the water extract after centrifugation, put it on the D201 macroporous adsorption resin column, first elute the impurities with water, and then elute with 70% ethanol, the dropping speed is 2.0ml / (min·cm 2 ), collect the ethanol eluate, and the collected amount is 4 times of column volume;

[0033] Refining: When the eluate is concentrated under reduced pressure to half, add activated car...

Embodiment 3-11

[0035] Based on 10kg of raw materials, the amount and composition of enzymes, as well as the time and temperature of enzymatic hydrolysis are shown in the table below:

[0036]

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PUM

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Abstract

The invention provides an extraction process of paeoniflorin, which relates to the field of drug extraction and solves the problems of low production efficiency, poor product purity and inapplicability of industrial production and large-scale production in the prior art. The extraction process of paeoniflorin adopts the combination of an enzymatic method and a non-alcoholic liquid phase extraction process and comprises the following steps: firstly adding enzyme into Chinese herbal medicines containing paeoniflorin active ingredients to carry out enzymolysis, adding appropriate amount of water to decoct the Chinese herbal medicines after enzymolysis and standing the Chinese herbal medicines for cooling; centrifugally separating extract at high speed, collecting and filtering supernatant liquid, collecting and concentrating filtrate, eluting condensate for decolourization on macroporous adsorption resin columns to prepare crude paeoniflorin products and refining the crude paeoniflorin products to obtain the paeoniflorin with purity of more than 98 percent. Because of adopting the enzymatic method, the extraction process of the paeoniflorin enables the active ingredients in the raw materials to be extracted sufficiently and easily. Because of adopting the non-alcoholic liquid phase extraction process, the whole extraction process of the paeoniflorin has the obvious advantages of high product purity, high yield, low costs of construction and production, simple and convenient operation, and the like.

Description

technical field [0001] The invention relates to the field of medicine extraction, and relates to a paeoniflorin extraction process. Background technique [0002] Paeoniflorin, also known as paeoniflorin, English name: Paeoniflorin, molecular weight 480.45, molecular formula: C 23 h 28 o 11 In the prior art, it is mostly extracted and refined from the root of Paeonia albiflora Pall, a plant of the family Ranunculaceae. It is hygroscopic amorphous brown powder (90% is off-white powder), tetraacetate is colorless needle crystal, melting point: 196°C. Paeoniflorin is stable in acidic environment (pH2~6), unstable in alkaline environment. Studies have found that paeoniflorin has significant analgesic, sedative, anticonvulsant, antispasmodic, coronary artery expansion, hypoglycemic, immune regulation, and antitumor effects. [0003] Due to the complex composition of Radix Paeoniae Alba and the influence of technology, the ethyl ester of more than 90% high-purity paeoniflori...

Claims

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Application Information

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IPC IPC(8): C07H17/04C07H1/08
Inventor 张守力
Owner 张守力
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