Method for fast obtaining regenerated plant for cayenne pepper anther culture

Abstract

The invention relates to a method for fast obtaining a regenerated plant for cayenne pepper anther culture. The method comprises the following steps: selecting an anther with microspores in a late uninucleate stage; positioning the anther at a low temperature of 3-5 DEG C for pretreatment which lasts 24-72 h; sterilizing a flower bud subject to the low temperature pretreatment with 75% of alcohol and 0.1% of mercury bichloride; cleaning the flower bud with sterile water; stripping the anther from the flower bud in a sterile condition; inoculating the anther in a culture medium comprising MS basic culture medium, 0.5-1.0 mg / L of NAA, 1.0-2.0mg / L of KT, 2 wt% of maltose, 0.8 wt% of agar and 0.5 wt% of activated carbon; carrying out heat shock treatment lasting for 6-10 days at a temperature of 33-37 DEG C; and continuing the culture at an illumination intensity of 2000 lx, an illumination time lasting 12 h and a room temperature of 25 DEG C till a complete plant with roots, sprouts and leaves is formed. The method can accomplish the formation of embryoids with large anthers and different types on one culture medium and obtain the regenerated plant. 7 weeks is needed in the process from the beginning of the anther culture to the fast obtaining of the regenerated plant. The method can be used for the case that haploids, dihaploids and other materials are obtained from cayenne pepper by means of the anther culture.

Description

technical field [0001] The invention belongs to the field of biotechnology sciences, in particular to a method for quickly obtaining regenerated plants by culturing capsicum anthers. Background technique [0002] Haploid refers to an individual or organization with the number of gamete chromosomes. Individuals composed of only one chromosome group are called haploid (D.F.I.Langlet, 1927), which is equivalent to half of diploid. It is of great significance in the basic theoretical research of crops and in the actual breeding practice. In genetic research, haploid is a good experimental material, especially in crop genetics and transgenic research. For example, the double haploid obtained after haploid doubling is a good material for the study of molecular markers and genetic mapping; using haploid cells as receptors, the transgenic material obtained after doubling is theoretically a stable group. In breeding practice, using haploids to obtain double haploid (DH) population...

AI Technical Summary

Problems solved by technology

Generally this pathway is undesirable because it produces plants with genetic variation and complex ploidy

At present, in the haploid induction process of pepper, the regenerated plants are mainly produced through the second way, which must undergo multiple transfers and replacement of medium types, which undoubtedly prolongs the cultivation time and increases the difficulty of cultivation, resulting in the regenerated plants being obtained. very low rate

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