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Preparation of universal toxin paper detection sensor and application thereof

A detection sensor, a general-purpose technology, used in instruments, measuring devices, scientific instruments, etc., can solve the problems of limiting the popularization and application of sensors, inconvenient life for ordinary citizens, and difficulty in using methods.

Inactive Publication Date: 2010-01-06
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The outbreak of cyanobacteria in Wuxi Taihu Lake in 2007 caused large-scale pollution of domestic water and drinking water in Wuxi City, which caused great inconvenience to the lives of ordinary citizens and caused great harm to the health of the people.
However, due to the high cost of preparation and the difficulty of using methods, the practical application of the developed sensor is limited.

Method used

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  • Preparation of universal toxin paper detection sensor and application thereof
  • Preparation of universal toxin paper detection sensor and application thereof
  • Preparation of universal toxin paper detection sensor and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Preparation of cyanotoxin carbon nanotube paper sensor

[0029] 1. Preparation of carbon nanotube PSS solution: disperse PSS in an aqueous solution to obtain a PSS aqueous solution with a mass concentration of 5%-20%. Disperse the water-soluble carbon nanotubes in the PSS aqueous solution to obtain a carbon nanotube PSS solution with a carbon nanotube concentration of 30-60 mg / mL, and perform ultrasonic treatment overnight in an ultrasonic instrument at a power of 100 W and 40 KHz.

[0030] 2. Add a certain concentration of MC-LR antibody solution to the carbon nanotube PSS solution prepared above to obtain an antibody-carbon nanotube PSS solution with a final concentration of MC-LR antibody of 5-30 μg / mL.

[0031] 3. Immerse the filter paper with a fixed length and width (5cm×0.5cm) (qualitative filter paper with a diameter of 18cm, Hangzhou Xinhua Paper Co., Ltd.) into the antibody-carbon nanotube PSS solution, and take it out after standing for 10-30 minute...

Embodiment 2

[0032] The rapid and highly sensitive detection method of embodiment 2 toxin

[0033] 1. With the above-mentioned prepared carbon nanotube paper sensor as the working electrode, the Pt electrode as the counter electrode, and the saturated calomel electrode as the reference electrode, record the current flowing through the working electrode with time under the control of the electrochemical workstation, namely i-t curve.

[0034] 2. Optimize the buffer solution used in the detection system, so that the response signal is the strongest in the specific buffer solution phosphate buffer solution PBS.

[0035] 3. Optimizing the pH value of the buffer solution of the detection system, so that the response signal of the sensor is the strongest under the specific PBS condition of pH 7.4.

[0036] 4. Optimize the temperature of the detection system, so that the sensor has the strongest response signal when the detection is carried out at a specific temperature of 37°C.

[0037] 5. Add...

Embodiment 3

[0041] Replace the type of antibody used in step 2 of Example 1 (such as ochratoxin antibody, aflatoxin antibody, antibiotic gentamycin antibody, etc.), prepare sensors for different toxins, and establish a general-purpose sensor preparation technology .

[0042] Optimize the detection conditions of general-purpose sensors, and establish detection methods and technical standards for general-purpose sensors.

[0043] Using the prepared general-purpose toxin paper detection sensor as the working electrode, replace the MC-LR series solution with the general-purpose toxin, and use the same conditions as the detection method of the cyanobacterial toxin paper detection sensor to establish the current-toxin concentration for detecting the general-purpose toxin Standard curve: detect the stable current value of the sample liquid, and compare it with the established current-toxin concentration standard curve to determine the content of the universal toxin in the detection sample liquid. ...

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Abstract

The invention relates to preparation of a universal toxin paper detection sensor and application thereof and belongs to the technical field of toxin detection. The preparation comprises the following steps: packaging the traditional filter paper by utilizing a DIP-Dry technology through a carbon nanometer tube of a functional nanometer material during the preparation of the toxin paper detection sensor and adding an antibody of cyanobacterial toxin MC-LR into package solution to prepare the paper detection sensor capable of specially detecting the cyanobacterial toxin. The universal toxin paper detection sensor has the detection sensitivity of 0.6 ng / mL satisfying the limiting requirements of WHO on MC-LR in daily water, has equivalent detection sensitivity compared with the traditional ELISA method and leads the whole detection process not to exceed 0.5 hour, thereby greatly reducing the detection time, ensuring the detection quality and remarkably improving the detection efficiency simultaneously. The method is a universal detection method and can be used for conveniently preparing another universal toxin paper detection sensor only through replacing the antibody added during the preparation process of the sensor.

Description

technical field [0001] The invention relates to the preparation and application of a general-purpose paper-based toxin detection sensor, relates to the generality of the detection method for toxin detection, and belongs to the technical field of toxin detection. Background technique [0002] With the development of society, environmental pollution is becoming more and more serious, and there are more and more toxins in water resources, which have caused great harm to the lives of ordinary residents. The outbreak of cyanobacteria in Wuxi Taihu Lake in 2007 caused large-scale pollution of domestic water and drinking water in Wuxi City, which caused great inconvenience to the lives of ordinary citizens and caused great harm to the health of the people. In 2009, the domestic water source in Yancheng, Jiangsu Province was polluted by chemical plants, forcing residents in many districts to cut off water supply in large areas. The pollution of water resources not only brings incon...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/403G01N33/53
Inventor 胥传来尼古拉斯·科托夫陈伟许定花刘丽强朱颖越孙凤霞彭池方胡拥明
Owner JIANGNAN UNIV
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