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Function labeling development of wide compatibility gene S5<n>

A gene, wide-parent technology, applied in the field of molecular genetics, can solve problems such as hybrid sterility, and achieve the effect of shortening the breeding process and improving the breeding efficiency

Inactive Publication Date: 2009-12-30
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The difference of only two bases between the S5 alleles of indica and japonica rice causes the substitution of two amino acids in the corresponding protein, which may be the reason for the hybrid sterility

Method used

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  • Function labeling development of wide compatibility gene S5&lt;n&gt;
  • Function labeling development of wide compatibility gene S5&lt;n&gt;

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1, a kind of identification broad affinity gene S 5 n Acquisition of Genotype Molecular Markers

[0022] 1 test material

[0023] Hybrid F of indica rice 3037 and japonica wide-compatibility variety 02428 bred by Jiangsu Agricultural College 1 and F 2 group.

[0024] 2DNA extraction

[0025] Take about 1 gram of rice leaves at the tillering stage or booting stage, and use the SDS method to extract DNA.

[0026] DNA extraction was slightly modified with reference to the method of Dellapporta et al. (1983), and the specific steps were as follows:

[0027] ①Take 200-300 mg of fresh rice leaves (leaves stored at -20°C), grind them into powder with liquid nitrogen in a pre-cooled mortar at -20°C.

[0028] ② Transfer to a 1.5ml centrifuge tube.

[0029] ③Add 600ul SDS extract solution, shake well, incubate at 65°C for 30min, shake 3-4 times.

[0030] ④Add 1 / 4 volume (about 100ul) of KAc and shake well.

[0031] ⑤Add 1 / 2 volume (about 300-400ul) of chlorof...

Embodiment 2

[0044] Example 2: Marker genotype analysis of rice variety resources using S5136 marker

[0045] Materials used for analysis include Asian cultivated rice (Oryza Sativa L.) (indica rice, japonica rice) and cultivated rice ancestor common wild rice (Oryza Rufipogen) and weedy rice materials; geographical distribution includes China, Japan, India, Korea, There are 550 species resources in the United States and Thailand. These materials include not only cultivated varieties popularized in a large area, but also local varieties, such as local varieties in the Taihu Lake Basin. Dular is a local variety from the Aus group in India. It has red skin. It has stronger affinity and affinity spectrum than 02428. It is a commonly used wide-compatibility gene resource. Other indica rice such as: 9311, IR24, IR36, Nanjing 11, Nanjing 14, Nanjing 16, IR58, Yangfuxian 2, Yangdao 1, Zhenxian 96, Guichao 2, Miryang 23, Hunan Zao, Zaoxiang No. 1, Zhongzao 9, Chengai 29, Jasmine Xiangxian, Yunna...

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Abstract

The invention relates to a molecular labeling method of a wide compatibility gene S5<n>, belonging to the field of molecular genetics. An InDel label S136 is designed according to a structural difference between the cloned wide compatibility gene S5<n> and a non-glutinous rice allelic gene Si and between the cloned wide compatibility gene S5<n> and a japonica rice allelic gene Sj and is separated from the wide compatibility gene S5<n> and the allelic genes thereof. The label can be utilized to accurately sieve and distinguish a material with the wide compatibility gene S5<n> from variety sources and can be used for the molecular labeling breeding of the wide compatibility gene S5<n> and the seed selection of a rice wide compatibility restorer.

Description

technical field [0001] The present invention provides broad affinity gene S 5 n The molecular marker identification method belongs to the field of molecular genetics and is specially used for the broad affinity gene S 5 n genotype identification. technical background [0002] Cultivated rice (Oryza sativa L.) is divided into two subspecies: indica rice (Oryza sativa L. indica sub) and japonica rice (Oryza sativa L. japonica sub), and inter-subspecies hybrids have a strong advantage. However, the hybrids between indica and japonica subspecies have fertility barriers, and the seed setting rate is very low, which limits the use of heterosis. In rice, there is a special kind of germplasm resources called wide-compatibility varieties, such as 02428 bred by Jiangsu Academy of Agricultural Sciences. The offspring hybridized with most indica and japonica rice are normal and fertile, and its wide-compatibility gene has been successfully cloned . The normal S5 gene encodes an as...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 杨杰仲维功陈志德王军
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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