Area decontamination via low-level concentration of germicidal agent
A bactericide, regional technology, applied in the fields of chemistry, atomized substances, water supply devices, etc., can solve the problems of insufficient, expensive, low efficiency, etc.
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Embodiment 1
[0055] The following experiment was performed to demonstrate the reduction of spores of G. stearothermophilus surviving on stainless steel surfaces using hydrogen peroxide vapor released from the urea complex at a minimal level of 0.6 ppm. Inoculate 10 μL of 6 × 10 4 Inoculum of spores. The inoculum then dries on the blade surface. Nine inoculated blades placed at 1ft 3 In a sealed chamber made of acrylic. In the chamber the urea complex was placed in a glass tube with fluid confinement enabling the peroxide concentration in the chamber to be controlled. Three similarly inoculated blades were placed in a covered petri dish and placed close to the chamber as a control. At three-day intervals, three inserts were removed from the sealed chamber along with a control. The inserts were subjected to standard restoration, plating and inoculation procedures from which surviving spore counts and log reduction values were determined. The log reduction values are shown in Table ...
Embodiment 2
[0060] To demonstrate that the log reduction of spores of B. stearothermophilus hydroperoxide is dependent on the concentration of peroxide, scalpel blades prepared as described in Example 1 were exposed to a minimum of 0.35 ppm H 2 o 2 concentration. In this case, the inoculated blades were exposed continuously, which resulted in some changes in the mean peroxide concentration during the exposure. The log reduction values after 3, 6, and 9 days of exposure and the average, high and low concentrations of peroxide during exposure are shown in Table 2.
[0061] Table 2
[0062] log-reduced mean
[0063] Concentrations shown above are averages over the entire exposure period. As shown, there was some variation at the very small concentration of 0.35 ppm, however, the data in the table indicate that higher concentrations resulted in higher microbial kill rates.
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