Rapid detection kit of legionella bacteria and detection method thereof

Abstract

The invention discloses a rapid detection kit of legionella bacteria, comprising bacteria genomic DNA extracting solution, PCR reaction solution, PCR product purification solution and restriction endonuclease solution; wherein the solutions are respectively placed in a container; the PCR reaction solution comprises a first PCR reaction solution and a second PCR reaction solution; and the first PCR reaction solution and the second PCR reaction solution are respectively placed in containers. The invention also discloses a detection method of the kit. The invention can be used for detecting not only clinical samples such as the legionella bacteria in sputum and bronchial lavage fluid, but also the legionella bacteria in water sample, and has the advantages of high speed, simple and convenient and easy usage.

Description

Technical field: [0001] The invention relates to the technical field of legionella species detection, in particular to a Legionella species that can not only detect Legionella in clinical and environmental water samples, but also can be classified into Legionella pneumophila and non-pneumophila Legionella Rapid detection kit and detection method thereof. Background technique: [0002] At present, Legionella detection kits are mainly divided into two categories. One is to use the antigen-antibody reaction to detect Legionella specific antibodies or antigens. Such kits include Legionella ELISA kits and Legionella colloidal gold kits, because Legionella-related antigens or antibodies appear late in patients with Legionella infection. It usually takes a week or more to detect, and at the same time, it can only detect Legionella pneumophila, and has a certain cross-reactivity, so this type of kit has certain limitations. The second category is to use molecular biology methods t...

AI Technical Summary

Problems solved by technology

One is to use the antigen-antibody reaction to detect Legionella specific antibodies or antigens. Such kits include Legionella ELISA kits and Legionella colloidal gold kits, because Legionella-related antigens or antibodies appear late in patients with Legionella infection. It usually takes a week or more to detect, and at the same time, it can only detect Legionella pneumophila, and has a certain cross-reaction, so this type of kit has certain limitations

The second category is to use molecular biology methods to detect Legionella, such as the fluorescent quantitative PCR detection kit for Legionella pneumophila, which can only be used to detect Legionella pneumophila, and cannot detect non-Legionella pneumophila

Although more than 80% of clinical Legionella infections are caused by Legionella pneumophila, and Legionella pneumophila is also widely present in the water environment, but non-pneumophila Legionella also has a relatively wide range of existence and certain infectivity

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