Method for preparing three-dimensional silk fibroin porous scaffold material

Abstract

The invention discloses a method for preparing three-dimensional silk fibroin porous scaffold material, and relates to the field of biomaterial, in particular to the field of tissue engineering scaffolds. The method comprises: putting silk fibroin water solution with the volume concentration of 2-50wt% into a container, and adding sodium chloride into the silk fibroin water solution at 20-80 DEG C under continuous stirring, wherein the ratio between the mass (unit: gram) of sodium chloride granules and the volume (unit: ml) of silk fibroin solution is 1-20:1; after gelatinating, standing and precipitating the silk fibroin solution, taking out the precipitate to be continuously stirred and evenly mixed; then, putting the precipitate into a mould with the needed shape to be dried; finally, washing off the sodium chloride in the scaffold by distilled water, and obtaining the silk fibroin porous scaffold material having three-dimensional porous structure. No organic solvent is used in the method, the method is simple and efficient, and the prepared silk fibroin porous scaffold has controllable porosity factor and pore size, highly communicated pore structure as well as degradability.

Description

Technical field [0001] The invention is a preparation method of a three-dimensional silk fibroin porous scaffold material, which relates to the field of biomaterials, in particular to the field of tissue engineering scaffolds. Background technique [0002] Silk fibroin is a natural polymer fibrin extracted from silk. Because of its excellent physical and chemical properties and biocompatibility, the research and application of silk fibroin in biomedical materials have received extensive attention in recent years. With the rise of tissue engineering and the demand for porous materials in tissue engineering, more and more attention has been paid to the preparation of silk fibroin scaffold materials that can be used in tissue engineering. [0003] In 1987, the Natural Science Foundation Engineering Council put forward the concept of tissue engineering at the Bioengineering Symposium, that is, "applying the principles and methods of engineering and life sciences to understand the relat...

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Problems solved by technology

However, the preparation of porous silk fibroin scaffolds by these methods basically requires a freeze-drying step to shape the silk fibroin, which is cumbersome; and the preparation process also requires the use of organic solvents such as hexafluoroisopropanol and methanol, which may cause the scaffold to have toxicity

In 2005, Kim UJ et al. reported a salt leaching method that completely prepared silk porous scaffolds from silk fibroin aqueous solution without using any organic solvent. We found after many repetitions that this method cannot effectively Prepare silk porous scaffolds, because the silk gel will dissolve together during the salt washing process

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