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Array electrode-based biosensor used for rapidly detecting minimal pesticide residue on fruit and vegetable surfaces

A biosensor and array technology, applied in the field of rapid detection of pesticide residues, can solve the problems of unfavorable instrument miniaturization, reduced instrument sensitivity, and small electrode response current, and achieve reliable detection results, improved detection sensitivity, and increased response current.

Inactive Publication Date: 2012-01-11
北京农产品质量检测与农田环境监测技术研究中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In practical applications, pesticide residues in agricultural products are trace or even trace amounts, and the response current of a single electrode is small, requiring special current detection instruments to meet the accuracy requirements, which is not conducive to the miniaturization of the instrument, and the weak signal response is easy to cause noise interference and baseline shift, reducing instrument sensitivity

Method used

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  • Array electrode-based biosensor used for rapidly detecting minimal pesticide residue on fruit and vegetable surfaces
  • Array electrode-based biosensor used for rapidly detecting minimal pesticide residue on fruit and vegetable surfaces
  • Array electrode-based biosensor used for rapidly detecting minimal pesticide residue on fruit and vegetable surfaces

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Rapid determination of organophosphorus pesticide residues on apple surface by array electrode biosensor

[0033] 1. Preparation of array electrode biosensor

[0034] On the electrode film substrate, first use screen printing technology to print conductive silver paste to form electrode connections and reference electrodes, and then print conductive carbon paste to cover the terminals to form working electrodes and counter electrodes. The connecting terminal, working electrode, counter electrode and reference electrode are bare conductive materials, and a layer of insulating ink is printed on the rest of the bare parts. Made of insulating film, 8 working electrodes are printed on it with conductive materials, a ring-shaped reference electrode in the center and a disc auxiliary counter electrode, except for all working electrodes, counter electrodes, reference electrodes and terminals, the rest Printed insulating ink overlay.

[0035] The resulting screen-printed elect...

Embodiment 2

[0048] Rapid determination of organophosphorus pesticide residues on apple surface by array electrode biosensor

[0049] 1. Preparation of array electrode biosensor

[0050] Using the screen electrode prepared in Example 1 as the base electrode:

[0051] (1) Disperse carbon nanotubes, glutaraldehyde and acetylcholinesterase in ethanol so that the concentration is 10 mg / ml, and fix acetylcholinesterase on the surface of the working electrode under the joint action of carbon nanotubes and glutaraldehyde to form Composite layer of carbon nanotubes and acetylcholinesterase;

[0052] (2) coating one deck of milky acrylic resin on the surface of above-mentioned composite layer, dry in the shade;

[0053] (3) Use a small volume of 0.05-0.1M phosphate buffer solution (pH 7.0) to drop on the surface of the acrylic resin to form a film of the buffer solution required for the electrochemical reaction on the biosensor.

[0054] 2. Determination of malathion on apple surface with array ...

Embodiment 3

[0063] Rapid Determination of Carbamate Pesticide Residues on Vegetable Leaf Surface with Array Electrode Biosensor

[0064] 1. Preparation of array electrode biosensor

[0065] Using the screen electrode prepared in Example 1 as the base electrode:

[0066] (1) 5 μl of carbon nanomaterials with a concentration of 1 mg / mL (using ethanol as a dispersant) was drip-coated on multiple array working electrodes, and dried under an infrared lamp;

[0067] (2) Apply 5 μl of butyrylcholinesterase solution (dissolved in PBS buffer) with a concentration of 2 U / ml on the electrochemical catalyst layer, add 5 μl of 1% glutaraldehyde solution dropwise, and dry;

[0068] (3) Use a microvolume (40μl) of 0.1M phosphate buffer solution (pH7.0) and an appropriate amount of alkylbenzenesulfonate (surfactant) to form a liquid, adsorb on the PE polymer material, and place it on the biosensor Forms buffer films required for electrochemical reactions.

[0069] The above-mentioned electrode is bent...

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Abstract

The invention relates to an array electrode-based biosensor, comprising insulating media, a reference electrode, an auxiliary electrode and a plurality of working electrodes, wherein, a plurality of working electrodes form an array, the surface of the biosensor has a buffer solution film formed by a macromolecule polymeric material layer and the absorbed buffer solution. Compared with the currentbiosensors, the biosensor in the invention has the main advantages that: 1. the detection result is reliable and accurate; array working electrodes are connected in parallel, the capture probability of the electric signals can be improved by the working electrode array, thus improving detecting reliability and reducing the side effects brought about by differences among electrodes and failure of the single electrode; 2. detection environment is self-carried; the detected samples need no pre-treatment, thus realizing rapid and nondestructive detection; 3. the parallel connection of array working electrodes improves the signal-to-noise ratio of the test system and the detection sensitivity, simultaneously, ordinary, microminiature and portable detectors can be used as the response current increases, and are applicable to field detection.

Description

technical field [0001] The invention relates to the field of rapid detection of pesticide residues, in particular to a biosensor for detecting trace pesticide residues on the surface of fruits and vegetables and an array electrode thereof. Background technique [0002] The wide application of pesticides in agricultural production has played an important role in increasing crop yield and income. However, the long-term large-scale use of pesticides has also caused excessive pesticide residues in soil and agricultural products, causing harm to the ecological environment and human health. The problem of pesticide residues is related to the safety of agricultural products, which has become a public health issue of widespread concern. Since the production and transportation of domestic agricultural products are very scattered, vegetable farmers can directly transport their products to the market for sale. This situation makes the safety and rapid detection of agricultural products...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/403G01N27/327
Inventor 潘立刚向玲韩平陆安祥王纪华
Owner 北京农产品质量检测与农田环境监测技术研究中心
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