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Method for producing deoxyadenosine by bioconversion method

A technology for deoxyadenosine and adenine, which is applied in the field of biotransformation to produce deoxyadenosine, which can solve the difficulties in effective contact between substrates and biological enzymes, affecting the rate and yield of conversion reactions, and the low water solubility of substrate adenine, etc. problems, to achieve low cost, mild conditions, and high selectivity

Inactive Publication Date: 2009-11-11
SHANDONG JIANWEI BIOENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The substrate adenine in this method has very low water solubility, which makes it very difficult to effectively contact the substrate with biological enzymes, which seriously affects the rate and yield of the conversion reaction; the catalytic deoxyribose transferase and the like are intracellular enzymes, microbial The lipid bilayer structure of the cell membrane makes it difficult for the substrate to enter and exit the microbial cell, and the reaction speed and conversion rate are low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1, biotransformation method produces deoxyadenosine

[0020] (1) Bacteria slant culture

[0021] Inoculate Lactobacillus helveticus (Lactobacillus helveticus) CGMCC 1.1877 (General Microbiology Center of China Microbiological Culture Collection Management Committee) into the slant culture medium, 37 ℃, pH value 6.5, and cultivate for 24 hours to make the slant strain; the slant medium consists of the following weight The composition of the serving:

[0022] 18 parts of glucose, 18 parts of peptone, 8 parts of yeast extract, 2 parts of ammonium citrate, 2 parts of dipotassium hydrogen phosphate, 9 parts of sodium sulfate, 0.02 parts of manganese sulfate, 20 parts of agar and 1000 parts of water.

[0023] (2) Inoculation fermentation

[0024] Insert the slant bacterial strain into the fermentation medium and expand the cultivation as the seed solution; the seed solution is inserted into the fermentor equipped with the fermentation medium at 10% (volume percen...

Embodiment 2

[0034] Embodiment 2, biotransformation method produces deoxyadenosine

[0035] (1) Lactobacillus helveticus CGMCC 1.1877 was inoculated into the slant culture medium, 40 DEG C, pH6.5, cultivated for 24 hours to make the slant strain; the slant medium was composed of the following components by weight:

[0036] 15 parts of glucose, 18 parts of peptone, 6 parts of yeast extract, 2 parts of ammonium citrate, 2 parts of dipotassium hydrogen phosphate, 8 parts of sodium sulfate, 0.02 parts of manganese sulfate, 20 parts of agar and 1000 parts of water.

[0037] (2) Inoculation fermentation

[0038] Insert the slant bacterial strain into the fermentation medium and expand the cultivation as the seed liquid; the seed liquid is inserted into the fermenter equipped with the fermentation medium with an inoculum of 20% (volume percentage) at 37°C, 140 rpm, and the pH value is 6.5, fermented for 36 hours; the fermentation medium was composed of the following components by weight:

[003...

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Abstract

The invention discloses a method for producing deoxyadenosine by a bioconversion method, comprising the following steps of: inoculating swiss lactobacillus seed liquid in a fermentation medium for fermentation and culture, thus obtaining a crude strain; crushing the crude strain to prepare a cell homogenates; and adding a deoxythymidine and a adenine in the cell homogenates for enzymatic reaction, thus obtaining the deoxyadenosine; the fermentation medium comprises the following substances by weight portion: 10-20 portions of dextrose, 1-5 portions of peptone, 10-25 portions of corn gluten, 1-2 portions of citric acid amino, 1-2 portions of potassium phosphate dibasic, 5-10 portions of sodium sulfate, 0.02-0.4 portions of manganese sulfate and 1000 portions of water; and the granularity of the adenine is less than or equal to 10 microns. The method for producing the deoxyadenosine by the bioconversion method sufficiently utilizes the high effectiveness, specificity and moderateness of the enzyme, and has the advantages of high selectivity, moderate condition, simple process, low cost, small pollution and the like compared with the traditional chemical synthesis method.

Description

technical field [0001] The invention relates to a method for producing deoxyadenosine by biotransformation. Background technique [0002] Jiang Zhongliang et al. reported the research on the total synthesis of 2'-deoxyadenosine (Journal of Tongji University: Natural Science Edition, 2007, 35(9): 1264-1268), Lu Youchang et al. reported the synthesis of 2'-deoxyadenosine ( Applied Chemical Industry, 2006, 35(7): 564-565), Wang Xiaomeng et al. reported the research on the synthetic route of 2'-deoxyadenosine related intermediates (dissertation), Henan Normal University and Xinxiang Tuosheng Chemical Technology Co., Ltd., etc. Obtained a process patent for the production of 2′-deoxyadenosine by chemical synthesis (Chinese patent 200710053920.X). The above documents all adopt chemical method to prepare deoxyadenosine. Put 6-chloroadenine and 1-acetyl-3,5-bis(p-methylbenzoyl)-2-deoxyribose and the organic solvent containing phosphate phenol ester compound catalyst into the react...

Claims

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Application Information

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IPC IPC(8): C12P19/40C12R1/225
Inventor 王大然乔瑞颖王雅婷
Owner SHANDONG JIANWEI BIOENG
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