A method for auxiliary screening of powdery mildew resistant wheat and its special primers

A technology for resistance to powdery mildew and wheat, which is applied in the fields of botanical equipment and methods, biochemical equipment and methods, and applications, and can solve problems such as hidden dangers in production, strong reproductive ability, and loss of resistance of major disease resistance genes.

Inactive Publication Date: 2011-12-21
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the complex physiological race of wheat powdery mildew, rapid variation, and strong reproductive ability, the resistance of the main disease resistance gene is rapidly lost, and the disease resistance of the variety cannot be stabilized for a long time, and there are great hidden dangers in production.

Method used

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  • A method for auxiliary screening of powdery mildew resistant wheat and its special primers
  • A method for auxiliary screening of powdery mildew resistant wheat and its special primers
  • A method for auxiliary screening of powdery mildew resistant wheat and its special primers

Examples

Experimental program
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Effect test

Embodiment 1

[0025] Example 1. Molecular markers Xbarc79 and Xwmc288 of powdery mildew resistance gene and acquisition of special primers thereof

[0026] The production of haploid wheat plants from wheat×maize crosses. Theor. Appl. Genet. 76: 393-397 was obtained by using Bainong 64 and Jingshuang 16 through the wheat×maize cross method (Laurie and Bennett, 1988, The production of haploid wheat plants from wheat×maize crosses. Theor.Appl. Genet. 76: 393-397) DH groups.

[0027] 1. Acquisition of phenotypes and their performance in the field

[0028]The powdery mildew race E20 (Duan et al. 1993, Establishment of wheat powdery mildew isolates with putative virulence genotype. Plant Prot. 19: 27-28), which is currently popular in wheat in my country, was used to test Bainong 64×Jingshuang 16DH containing 181 families. The populations were identified for adult plant resistance in 2005-06 and 2007-08 in Beijing and Anyang, respectively. The results showed that the maximum disease severity (MD...

Embodiment 2

[0043] Example 2, application of molecular markers Xbarc79 and Xwmc288 and their special primers

[0044] 1. Detect whether different strains and varieties of wheat carry molecular markers Xbarc79 and / or Xwmc288

[0045] Using the DH population line produced by the hybrid offspring of Bainong 64 and Jingshuang 16 and the genomic DNA of the wheat variety to be tested as templates, the primer pair Xbarc79-1 and Xwmc288-1 were used for PCR amplification.

[0046] The 15 μl PCR reaction system consists of: 20ng template DNA, 450 μM dNTPs (purchased from Tiangen Company), 0.95U Taq DNA polymerase (purchased from Tiangen Company), 1.5 μl 10×PCR buffer (purchased from Tiangen Company) Company), 4pmol primer pair Xbarc79-12.3μl, 4pmol primer pair Xwmc288-12.3μl, supplement the reaction system with sterile distilled water to 15μl. The procedure of PCR amplification was as follows: pre-denaturation at 94°C for 5 minutes; then denaturation at 94°C for 1 minute, annealing at 52°C for 1 m...

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Abstract

The invention discloses a powdery mildew resistant wheat auxiliary screening method and a special primer thereof. The powdery mildew resistant wheat auxiliary screening primer pairs provided by the invention are at least one pair of the primer pairs of Xbarc79-1 and Xwmc288-1; the deoxyribonucleotide sequence of the primer pairs Xbarc79-1 is shown as sequence 1 and sequence 2 in a sequence table; the deoxyribonucleotide sequence of the primer pair Xwmc288-1 is shown as sequence 3 and sequence 4 in the sequence table. The invention also provides molecular markers Xbarc79 and Xwmc288 of powdery mildew resistant gene, the deoxyribonucleotide sequence of the molecular marker Xbarc79 is shown as sequence 5 in the sequence table, and the deoxyribonucleotide sequence of the molecular marker Xwmc288 is shown as sequence 6 in the sequence table. The primer pairs and the molecular marker of the invention can play an important role in wheat breeding for disease resistance.

Description

technical field [0001] The invention relates to a method for assisting in screening wheat resistant to powdery mildew and special primers thereof. Background technique [0002] Wheat powdery mildew is a fungal disease caused by wheat powdery mildew (Blumeria graminis f.sp.tritici), which mainly occurred in mild climate, humid and rainy areas in the past. With the popularization of dwarf and semi-dwarf varieties of wheat and the continuous improvement of water and fertilizer conditions, the production level of wheat has been greatly improved. The high density caused by large sowing volume, unreasonable cultivation measures such as partial application of nitrogen fertilizer, excessive irrigation, etc., artificially The induction condition of powdery mildew is provided, and the harm of powdery mildew is aggravated day by day. Before the 1970s, wheat powdery mildew was mainly prevalent in the humid and rainy southwest regions such as Yunnan and Guizhou and the coastal areas of ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68C12N15/11A01H1/04
Inventor 夏先春何中虎兰彩霞
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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