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Lactobacillus fermentum ess-1, dsm17851 and its use for the treatment and/or prevention of candidiasis and urinary tracrinfections

A technology of DSM17851 and Lactobacillus fermentum, applied in the field of probiotics, can solve problems such as limited success of vaginal candidiasis

Inactive Publication Date: 2009-10-28
ESSITY HYGIENE & HEALTH AB
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there has been very limited success in preventing and / or treating vaginal candidiasis

Method used

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  • Lactobacillus fermentum ess-1, dsm17851 and its use for the treatment and/or prevention of candidiasis and urinary tracrinfections
  • Lactobacillus fermentum ess-1, dsm17851 and its use for the treatment and/or prevention of candidiasis and urinary tracrinfections
  • Lactobacillus fermentum ess-1, dsm17851 and its use for the treatment and/or prevention of candidiasis and urinary tracrinfections

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0069] Example 1: Isolation and typing of Ess-1

[0070] Purpose

[0071] The initial aim of this study was to isolate and type Lactobacillus strains that inhibited the growth of C. albicans and C. glabrata to a greater extent than other Lactobacillus strains.

[0072] method

[0073] yeast strain

[0074] Clinical isolates of C. albicans and C. glabrata were used as test strains. These strains were isolated from the vaginas of women with vaginal candidiasis and healthy women.

[0075] Screen I

[0076] Approximately 140 Lactobacillus strains derived from human skin, throat, teeth, infant feces, vegetables and seeds were grown in MRS broth and streaked on MRS agar plates. The agar plates were incubated at 37°C under anaerobic conditions. Additionally, SAB (Sabouraud) agar (LAB M, Bury, UK) was poured over the MRS agar and allowed to solidify. A culture of Candida albicans was inoculated on this agar, and the plate was incubated anaerobically at 37°C. Inhibition was ass...

Embodiment 2

[0091] Embodiment 2: heat treatment

[0092] Purpose

[0093] The purpose of this experiment was to study the heat resistance of Candida growth inhibitory substances produced by strain Ess-1.

[0094] method

[0095] The LCF was heat-treated at 90°C for 20 minutes. Add Candida albicans and Candida glabrata to a final concentration of approximately 5 x 10 4 CFUml -1 . The plates were incubated at 37°C for approximately 20 hours and the vials were visually assessed for Candida growth and graded from 5 to 0. The plates and vials are as above.

[0096] result

[0097] The heat-treated filtrate of strain Ess-1 was equally successful in inhibiting yeast growth compared to the untreated filtrate used as a comparison. Therefore, heat treatment of the filtrate does not destroy the active substances of this bacterium.

Embodiment 3

[0098] Example 3: Inhibition of Candida albicans with LCF by adding salt and acid

[0099] Purpose

[0100] The aim of this study was to determine whether salt and acid affect the growth of Candida and whether an improvement in growth inhibition could be achieved when added to the LCF of strain Ess-1.

[0101] method - salt

[0102] LCF aliquots were pH adjusted to the pKa value for each salt and air dried until completely dry before resuspension in 0.3% sodium acetate and sodium propionate. Add Candida albicans to a final concentration of approximately 5 x 10 4 CFU / ml. Fractions were incubated at 37°C for 20 hours. Inhibition was assessed by visual turbidity and graded by two people using a template from a 5 to 0 scale. Wells with robust growth of Candida in pure dMRSs broth were graded 5, whereas wells with no observed growth were graded 0.

[0103] method - acid

[0104] Add succinic acid, acetic acid, propionic acid, and ascorbic acid to the tube containing LCF to a...

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Abstract

The present invention relates to a novel strain of Lactobacillus fermentum, denoted strain Ess-1, which has been deposited at Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH and has been assigned accession number DSM17851. Lactobacillus fermentum, strain Ess-1, has shown valuable properties in the inhibition of Candida. The invention therefore also relates to the medical use of Lactobacillus fermentum strain Ess-1, in particular for the treatment and / or prevention of candidasis and urinary tract infections. The invention also relates to pharmaceutical products and articles comprising this novel bacterial strain.

Description

technical field [0001] The present invention relates to the field of probiotics having a beneficial effect on candida infections, ie candidiasis, as well as bacterial infections. The invention also relates to pharmaceutical compositions and products for personal care comprising such bacteria and to pharmaceutical applications of such bacteria. Background technique [0002] The normal flora in the urogenital zone consists of a complex ecosystem comprising more than 50 different bacterial populations (Hill et al., Scand. J. Urol. Nephrol. 1984(86(suppl.)): 23-29). The normal flora is mainly bacteria belonging to the genus Lactobacillus (LB), which are Gram-positive bacilli suitable for the vaginal environment of women of childbearing age. These bacteria also help maintain the special environment and ecological balance in the vagina. The pH of the vaginal environment is below 4.5. Yeast and enterobacteria are less or absent (Redondo-Lopez et al, Rev. Inf. Dis. 1987(12):856-8...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/74C12R1/225A61K35/747
CPCC12N1/20A61K35/747C12R1/225A61P1/02A61P1/04A61P13/02A61P15/02A61P31/00A61P31/04A61P31/10C12N1/205C12R2001/225
Inventor U·胡斯马克U·福斯格伦布鲁斯克E·格兰霍坎松D·伦奎斯特
Owner ESSITY HYGIENE & HEALTH AB
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