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Culture medium for fermenting pleocidin producing bacteria

A spinosad and culture medium technology, applied in fermentation, microorganism-based methods, bacteria, etc., can solve the problems of limited sources, high cost of raw materials, low fermentation units, etc., to achieve increased fermentation units, foam elimination, and rich sources Effect

Inactive Publication Date: 2009-10-21
SHANGHAI INST OF PHARMA IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the existing technology, the fermentation medium of spinosad is not effective, and the fermentation unit is low, which cannot meet the needs of industrialization, and there are problems such as high cost of raw materials and limited sources, and it cannot be effectively controlled during the fermentation process. Eliminate foam, the fermentation broth often contains polysaccharides that are not fully utilized, resulting in a viscous fermentation broth that does not use subsequent extraction steps

Method used

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  • Culture medium for fermenting pleocidin producing bacteria
  • Culture medium for fermenting pleocidin producing bacteria
  • Culture medium for fermenting pleocidin producing bacteria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Embodiment 1: Determination of the optimal formulation of the medium of the present invention

[0021] 1): Determination experiment of single carbon source type

[0022] Method: Taking the formula of the basic fermentation medium as the control, the nitrogen source composition and dosage were unchanged, and glucose, starch, soluble starch, dextrin, fructose, lactose, maltose, sucrose, mannitol and other multi-carbon sources were added to conduct single-factor carbon In the source experiment, the addition amount of each carbon source is equivalent, both are 6%. The shake flask was fermented for 8 days, and the titer was determined by HPLC.

[0023]

[0024] 2): Carbon source combination experiment

[0025] Based on the above results, glucose was selected as the quick-acting carbon source and combined with other late-acting carbon sources. The shake flask was fermented for 8 days, and the titer was determined by HPLC.

[0026]

[0027] 3): Glucose addition exper...

Embodiment 2

[0052]Utilize spinosad to produce bacterium Saccharopolyspora spinosa to ferment, and fermentation medium is configured according to the data listed in the following table (percentage by weight):

[0053] Glucose 5.0, cottonseed meal 2.0, fish meal peptone 1.0, sunflower oil 2.0, lysine 0.2, CaCO 3 0.3 and tap water 89.5.

[0054] Sterilize at 121°C for 30 minutes. The inoculum size is 10%, the filling volume is 100ml / 750ml, and it is placed in a constant temperature room at 28°C with a rotary shaker at 250rpm for 8 days. After 8 days of fermentation, take 2ml of fermentation broth, centrifuge to get mycelia, soak in 6ml of acetone overnight, and then centrifuge to get The titer of the supernatant was determined by HPLC, and the fermentation unit was 536mg / L.

Embodiment 3

[0056] Utilize spinosad to produce bacterium Saccharopolyspora spinosa to ferment, and fermentation medium is configured according to the data listed in the following table (percentage by weight):

[0057] Glucose 8.0, cottonseed meal 3.0, fish meal peptone 2.0, sunflower oil 2.0, lysine 0.1, CaCO 3 0.5 and tap water 84.4.

[0058] Sterilize at 121°C for 30 minutes. The inoculum size is 10%, the filling volume is 100ml / 750ml, and it is placed in a 28°C constant temperature room with a rotary shaker at 250rpm for 10 days. After 10 days of fermentation, take 2ml of the fermentation broth, centrifuge to get the mycelia, soak in 6ml of acetone overnight, and then centrifuge to get The titer of the supernatant was determined by HPLC, and the fermentation unit was 1150 mg / L.

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Abstract

The invention provides a culture medium for fermenting pleocidin producing bacteria by using Saccharopolyspora spinosa NRRL18395 as strains, which comprises the following components: 5.0-9.0 percent of carbon source, 3.0-7.0 percent of nitrogen source, 0.2-2.5 percent of seed oil, 0.1-0.5 percent of amino acid, 0.1-1.0 percent of CaCO3 and 81.0-91.6 percent of water. The fermentation level of the pleocidin producing bacteria can be improved by 19 times to the maximum based on the original fermentation level, and thereby the culture medium for fermenting pleocidin producing bacteria is beneficial to the industrialization of pleocidin in China.

Description

technical field [0001] The invention belongs to the technical field of biological pesticide production, and in particular relates to a medium for fermenting spinosad-producing bacteria. Background technique [0002] Spinosad is a novel macrolide ultra-high-efficiency biological source extracted from the fermentation broth of Saccharopolyspora spinosa isolated from the soil of the Virgin Islands in 1982 by researchers from the American Lilly Company. pesticides. Produced by the Dow Agricultural Sciences Company of the United States, the trade names registered in my country in 1999 are Tracer (48% suspension) and Caixi (Success 2.5% suspension), which are respectively used in the control of diamondback moth and cotton bollworm. Its active ingredients are Spinosyn A and D, wherein the proportions of A and D are 85% and 15% respectively. Spinosad is mainly used in the control of cotton bollworm, tobacco budworm, diamondback moth, fruit fly, beet armyworm, thrips, etc. It is su...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P19/62C12R1/01
Inventor 李继安韩丽敏
Owner SHANGHAI INST OF PHARMA IND
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