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Effective component of cynanchum atratum and preparation method thereof

An effective component, the technology of Baiwei, is applied in the field of traditional Chinese medicine extracts for the treatment of tumor diseases, and can solve problems such as side effects and involvement of normal cells

Inactive Publication Date: 2009-10-07
TIANJIN TASLY PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the treatment of malignant tumors in the industry is mainly based on surgery, radiotherapy, and chemotherapy, but many chemical anticancer drugs often involve normal cells when they act on target cells, causing serious side effects

Method used

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  • Effective component of cynanchum atratum and preparation method thereof
  • Effective component of cynanchum atratum and preparation method thereof
  • Effective component of cynanchum atratum and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0110] The preparation of embodiment 1 Baiwei effective components

[0111] Pulverize Baiwei, pass through a 20-mesh sieve, add 8 times the amount of ethyl acetate:ethanol = 1:1, heat and reflux for 1 hour, extract twice, add 8 times the amount of 70% ethanol to the dregs, heat and reflux for 1 hour, and extract 2 times, the filtrate was combined to obtain the extract, after the extract was concentrated, the sample was dissolved with 5% ethanol, and the ODS-C18 column was crossed. At first, 1250ml of 5% ethanol was used as the mobile phase, and then 1250ml of 50% ethanol was replaced as the mobile phase to obtain Eluent, continue to separate eluent by preparative liquid chromatography, separation conditions: chromatographic column is Agilent preparative column (Zorbax SB-C18; 21.2mm×250mm), mobile phase is water A and acetonitrile B, gradient elution procedure is as follows

[0112]

[0113]

[0114] The flow rate is 10ml / min, the column temperature is room temperature; ...

Embodiment 2

[0115] The analysis of embodiment 2 Baiwei effective components

[0116] Chromatographic conditions Chromatographic column Agilent Zorbax SB-C18 column (4.6mm * 150mm, 5 μ m); Adopt gradient elution, mobile phase A phase is 0.2% glacial acetic acid aqueous solution, mobile phase B phase is the acetonitrile solution containing 0.2% glacial acetic acid; Gradient elution The desorption procedure is as follows: at 0 minutes, mobile phase A is 90% 0.2% glacial acetic acid aqueous solution, mobile phase B is 10% 0.2% glacial acetic acid acetonitrile solution; at 10 minutes, mobile phase A is 50% 0.2% glacial acetic acid Acetic acid aqueous solution, mobile phase B is the acetonitrile solution of 0.2% glacial acetic acid of 50%; In 30 minutes, mobile phase A is 0.2% glacial acetic acid aqueous solution of 5%, mobile phase B is the acetonitrile solution of 95% 0.2% glacial acetic acid; At 35 minutes, the mobile phase A was 5% 0.2% glacial acetic acid in water, and the mobile phase B...

Embodiment 3

[0119] Embodiment 3 Baiwei active ingredient preparation

[0120] Take the active components 1, 2, 3, 4, 5 or 6 of Baiwei in Example 1, mix 0.5g with 10.5g polyethylene glycol-6000 evenly, heat and melt, and move to the drip irrigation after the material is changed. Drop the liquid into liquid paraffin at 6-8°C, remove oil, and make 400 dropping pills.

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Abstract

The invention relates to an effective component of cynanchum atratum as well as a preparation method and an application thereof. The effective component of the cynanchum atratum is prepared according to the following steps: (1) a mixture of ethyl acetate and ethanol is used as a solvent to extract the cynanchum atratum; (2) dregs of a decoction are extracted with the ethanol so as to obtain an extracting solution; (3)the extracting solution is processed by chromatography through a chromatographic column to obtain an eluent; and (4) the eluent is eluted by prepared liquid chromatogram gradient and collected for 40.0-44.0 minutes, 44.0-48.0 minutes, 48.0-52.0 minutes, 52.0-56.0 minutes, 56.0-60.0 minutes and 60.0-64.0 minutes to obtain the effective component by using water and acetonitrile as flow phases.

Description

technical field [0001] The invention relates to a traditional Chinese medicine extract for treating tumor diseases, in particular to an effective component extracted from Baiwei, a preparation and a preparation method and use thereof. Background technique [0002] Tumor is a common and frequently-occurring disease, among which malignant tumor is the most serious disease that endangers human health. At present, the treatment of malignant tumors in the industry is mainly based on surgery, radiotherapy, and chemotherapy. However, many chemical anticancer drugs often involve normal cells when they act on target cells, causing serious side effects. The genotoxicity of botanical medicine is not obvious, and Chinese herbal medicine has unique advantages and broad application prospects in anti-cancer and anti-mutation, and Chinese medicine also plays a role that cannot be ignored in the adjuvant treatment of tumors. Paclitaxel is a typical natural compound obtained from plants with...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/27A61P35/00A61K125/00
Inventor 贺庆刘雳程翼宇水文波窦静葛志伟
Owner TIANJIN TASLY PHARMA CO LTD
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