Effective component of cynanchum atratum and preparation method thereof
An effective component, the technology of Baiwei, is applied in the field of traditional Chinese medicine extracts for the treatment of tumor diseases, and can solve problems such as side effects and involvement of normal cells
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Embodiment 1
[0110] The preparation of embodiment 1 Baiwei effective components
[0111] Pulverize Baiwei, pass through a 20-mesh sieve, add 8 times the amount of ethyl acetate:ethanol = 1:1, heat and reflux for 1 hour, extract twice, add 8 times the amount of 70% ethanol to the dregs, heat and reflux for 1 hour, and extract 2 times, the filtrate was combined to obtain the extract, after the extract was concentrated, the sample was dissolved with 5% ethanol, and the ODS-C18 column was crossed. At first, 1250ml of 5% ethanol was used as the mobile phase, and then 1250ml of 50% ethanol was replaced as the mobile phase to obtain Eluent, continue to separate eluent by preparative liquid chromatography, separation conditions: chromatographic column is Agilent preparative column (Zorbax SB-C18; 21.2mm×250mm), mobile phase is water A and acetonitrile B, gradient elution procedure is as follows
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[0114] The flow rate is 10ml / min, the column temperature is room temperature; ...
Embodiment 2
[0115] The analysis of embodiment 2 Baiwei effective components
[0116] Chromatographic conditions Chromatographic column Agilent Zorbax SB-C18 column (4.6mm * 150mm, 5 μ m); Adopt gradient elution, mobile phase A phase is 0.2% glacial acetic acid aqueous solution, mobile phase B phase is the acetonitrile solution containing 0.2% glacial acetic acid; Gradient elution The desorption procedure is as follows: at 0 minutes, mobile phase A is 90% 0.2% glacial acetic acid aqueous solution, mobile phase B is 10% 0.2% glacial acetic acid acetonitrile solution; at 10 minutes, mobile phase A is 50% 0.2% glacial acetic acid Acetic acid aqueous solution, mobile phase B is the acetonitrile solution of 0.2% glacial acetic acid of 50%; In 30 minutes, mobile phase A is 0.2% glacial acetic acid aqueous solution of 5%, mobile phase B is the acetonitrile solution of 95% 0.2% glacial acetic acid; At 35 minutes, the mobile phase A was 5% 0.2% glacial acetic acid in water, and the mobile phase B...
Embodiment 3
[0119] Embodiment 3 Baiwei active ingredient preparation
[0120] Take the active components 1, 2, 3, 4, 5 or 6 of Baiwei in Example 1, mix 0.5g with 10.5g polyethylene glycol-6000 evenly, heat and melt, and move to the drip irrigation after the material is changed. Drop the liquid into liquid paraffin at 6-8°C, remove oil, and make 400 dropping pills.
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