Method for detecting glyoxylate and glyoxal by high performance liquid chromatography

Abstract

The invention discloses a method for detecting glyoxylate and glyoxal by high performance liquid chromatography (HPLC), particularly a method for detecting the content of glyoxylate and glyoxal by HPLC in glyoxylate sample solution which is prepared from reaction solution for the oxidation preparation of glyoxylate from glyoxal by adding sodium hydroxide solution to the reaction solution, so as to carry out the disproportionation reaction between incompletely reacted glyoxal and the sodium hydroxide solution in the reaction solution. By adopting the method of the invention, the content of glyoxal as the raw material and glyoxylate as the product can be detected rapidly and accurately; and the invention has the advantages of simple detection method and good repeatability, therefore, the invention is applicable to the quality control over glyoxylate during the industrialized oxidation preparation process of glyoxylate from glyoxal.

Description

technical field [0001] The invention relates to a high performance liquid chromatography detection method for glyoxylic acid and glyoxal. Background technique [0002] Glyoxylic acid, also known as diglycolic acid or formylformic acid, has both carboxyl and aldehyde groups in its molecule, and its chemical properties are very active. As an important chemical raw material and an intermediate in organic synthesis, glyoxylic acid is widely used in the production of fine chemicals such as spices, medicines, pesticides, daily chemical additives, and water treatment agents. In recent years, the development and utilization of glyoxylic acid and its downstream products have shown broad market prospects. [0003] Zhou Xin et al. (Zhou Xin, Zhao Zhichao, Zhang Yanjie, etc., Discussion on the mechanism of separation of glyoxylic acid derivatives by reversed-phase ion-pair high performance liquid chromatography, Journal of Qingdao Institute of Chemical Technology, 1998, 19 (1): 67-69) ...

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Problems solved by technology

In this method, the reaction between glyoxylic acid and 2,4-dinitrophenylhydrazine is completely converted into glyoxylic hydrazone for a long time, and the signal interference and spectral overlap phenomenon of more complex systems cannot be overcome.

[0004] In the method of glyoxylic acid oxidation to glyoxylic acid, the quantitative detection of the main product of glyoxylic acid is of great significance to the evaluation of the reaction process and production guidance. Since the molecular structures of glyoxylic acid and glyoxal are very similar, both contain carboxyl or Aldehyde groups, the mutual interference between the raw material glyoxylic acid and the main product glyoxal is very large, and there is no efficient detection method for glyoxylic acid and glyoxal suitable for industrial processes

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