Compound enzyme for producing reducing sugar by degrading maize straws

Abstract

The invention provides a compound enzyme for producing reducing sugar by degrading maize straws. The compound enzyme is prepared from pectase, cellulose, hemicellulase, ligninolytic enzyme, amylase, prolease, lipase and chitosanase. Compared with the prior art, 1) the yield of the reducing sugar by degrading the maize straws has high yield, and the gross amount of the reducing sugar of straws pergram reaches over 480 mg, so the compound enzyme has wide application prospect in the fuel alcohol industry; 2) compared with sugar preparation by acid process, severe acid pollution is prevented to facilitate subsequent fermentation; and 3) compared with monomer cellulose or simple compound enzyme, the pretreatment of steam explosion with high energy consumption is not required, and the amount of the reducing sugar is increased by over 1 time; meanwhile, glucose and xylose in a degradation product have high content, side products are few so as to contribute to subsequent fermentation, the industrial application value of the maize straws is improved, and the compound enzyme can be applied to production of single cell protein and fuel alcohol.

Description

A complex enzyme that degrades corn stalks to produce reducing sugars technical field The invention belongs to the technical field of biological enzyme engineering, and in particular relates to a special composite enzyme for degrading reducing sugar produced by corn stalks. Background technique Straw cellulose is the most abundant renewable resource in the world, and it is a potential reserve resource for food, feed and energy industries. The annual output of corn stalks in my country is more than 200 million tons. Corn stalks are rich in cellulose, hemicellulose and lignin, and also contain some pectin, starch, protein and other substances. Among them, cellulose accounts for about 30% to 40%, which is a long-chain macromolecule formed by 8000 to 12000 glucose monomers connected by β-1,4-glycosidic bonds; hemicellulose is mainly composed of xylose and a small amount of arabinose , galactose, mannose, glucose and other components, accounting for about 35% to 48% of the dr...

AI Technical Summary

Problems solved by technology

The purpose of the present invention is to solve the problem of serious pollution of corn stalk chemical pretreatment, high energy consumption of steam explosion treatment, low enzymatic hydrolysis efficiency and incomplete enzymatic hydrolysis of single enzyme or simple compound enzyme, and solve the problem of reducing sugar production in the process of corn stalk degradation. Low efficiency and lack of practical application value, providing a compound enzyme for degrading reducing sugar produced by corn stalks