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Preparation method of tuber pinellia polyploid plant

A technology of polyploidy and pinellia, applied in the field of plant cell engineering, can solve problems such as reverting mutations, increasing the workload and complexity of screening mutant plants, and easily generating chimeras

Inactive Publication Date: 2009-09-09
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Germinated seeds, seedlings, buds, callus and shoot apex tissues are often used as colchicine treatment materials for polyploid induction by such methods, and the treatment methods include dipping, smearing, dripping and injection, etc. Chromosome doubling induction is easily disturbed by the physiological conditions of the processed materials, and chimeras are prone to occur, and back mutations may occur, thereby increasing the workload and complexity of mutant plant screening

Method used

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  • Preparation method of tuber pinellia polyploid plant
  • Preparation method of tuber pinellia polyploid plant
  • Preparation method of tuber pinellia polyploid plant

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] Embodiment 1, a kind of preparation method of peach leaf type pinellia polyploid plant, carries out following steps successively:

[0065] 1), soak the tender peach-leaf-type pinellia leaves with 75% alcohol by volume concentration for 30-45s, and then soak them with HgCl with a mass concentration of 0.1%. 2 Disinfect the solution for 1-5 minutes, rinse with sterile water for 5-10 times; cut the sterilized leaves into 0.5cm 2 The left and right small pieces were inoculated into the callus induction medium for 14-16 days of light culture, the light time was 12h / d, the light intensity was 1500lx-2000lx, and the culture temperature was 22-28°C to obtain yellow-green hard callus;

[0066] The formula of callus induction medium is: MS medium + 6-BA 1.0mg·L -1 +NAA 0.02mg·L -1 + sucrose 30g·L -1 , pH 5.6~6.0.

[0067] 2), transfer the hard callus to the callus subculture medium for light culture, the light time is 12h / d, the light intensity is 1500lx-2000lx, and the cultu...

Embodiment 2

[0086] Embodiment 2, polyploid induction is carried out with peach-leaf pinellia as material, the specific steps are as shown in Example 1, the difference is that the mass concentration of colchicine in step 5 is 0.003%, the treatment time is 48h, and the induction obtains The polyploid traits of the treatment were consistent with Example 1, and the polyploid induction rate of this treatment was 31.43%.

Embodiment 3

[0087] Example 3, polyploid induction was carried out with peach-leaf pinellia as a material, the specific steps were as shown in Example 1, the difference was that the mass concentration of colchicine in step 5 was 0.005%, the treatment time was 24h, and the induction obtained The polyploid traits of the treatment were consistent with Example 1, and the polyploid induction rate of this treatment was 33.15%.

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Abstract

The invention discloses a preparation method of a tuber pinellia polyploid plant. In the method, tuber pinellia leaves are used for inducing callus with loose texture, then the callus is treated by suspension culture, tuber pinellia single cell is separated out to be processed by colchicines, then the tuber pinellia single cell is cultured in a tuber pinellia single cell culture medium after the colchicines is washed away, the formed cell mass is inoculated into the callus inducing culture medium for culturing, the formed callus is moved into a cluster buds culture medium for culturing and then moved into a rooting medium for rooting medium after the plant leaves are completed spread, and the root tips are taken for chromosome observation for confirming the ploidy of the plant, so as to obtain the tuber pinellia polyploid plant. The tuber pinellia polyploid plant obtained by the method has obvious polyploidy feature and the screening process is relatively simple.

Description

technical field [0001] The invention belongs to the field of plant cell engineering, and in particular relates to a method for inducing polyploids of medicinal plants Pinellia polyploidy. Background technique [0002] Pinellia spp. is a plant of the genus Pinellia in the family Araceae, which mainly reproduces asexually. The dried tubers are common traditional Chinese medicines. Modern pharmacological studies have shown that pinellia has many functions such as drying dampness and resolving phlegm, reducing adverse events and relieving cough, eliminating mass and dissipating stagnation, anti-early pregnancy, anti-tumor effect, lowering blood fat, protecting liver and treating coronary heart disease. At present, wild Pinellia resources are gradually decreasing due to people's blind digging, while the genetic background and ploidy of artificially cultivated Pinellia are complex and changeable, and the heterogeneity of germplasm resources limits the industrialization of Pinellia...

Claims

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Application Information

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IPC IPC(8): A01H1/08A01H4/00C12N5/04
Inventor 陈集双郭巧萍
Owner NANJING UNIV OF TECH
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