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Bacillus amyloliquefaciens capable of degrading benzene compounds and application thereof

A technology for dissolving starch spores and benzene-based compounds, applied in the field of biological treatment of benzene-based compounds, can solve the problems such as BTEX that have not been degraded, and achieve the effect of high-efficiency degradation

Inactive Publication Date: 2009-08-26
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] After retrieving relevant patent documents, only found that there is a report about Bacillus bataviensis PBACGMCC No.1092 denitrifying and degrading benzene under anaerobic conditions, and there is no report of using Bacillus amyloliquefaciens to degrade BTEX under aerobic conditions in the present invention

Method used

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  • Bacillus amyloliquefaciens capable of degrading benzene compounds and application thereof
  • Bacillus amyloliquefaciens capable of degrading benzene compounds and application thereof
  • Bacillus amyloliquefaciens capable of degrading benzene compounds and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1. Isolation, purification and identification of Bacillus amyloquefaciens byf-5

[0029] 1. Isolation and purification of Bacillus amyloquefaciens byf-5

[0030] Bacillus amyloquefaciens byf-5 is a Gram-positive bacterium obtained by sampling from the activated sludge in the aeration tank of Zhejiang Zhenhai Refining and Chemical Wastewater Treatment Plant, after domestication, isolation and purification. Specific steps are as follows:

[0031] (1) Directed domestication of sludge

[0032] Activated sludge from the aeration tank of Zhejiang Zhenhai Refining and Chemical Wastewater Treatment Plant was taken. The activated sludge was subjected to directional domestication of benzene series compounds (BTEX), and every 5 days was a domestication cycle, and the domestication period was 3 months until the activated sludge achieved stable and efficient degradation of benzene series compounds.

[0033] (2) Screening and purification of bacterial strains

[0034] The...

Embodiment 2

[0044] Example 2.Bacillus amyloquefaciens byf-5 detects BTEX biodegradability

[0045] 1. Biodegradation characteristics of Bacillus amyloliquefaciens byf-5 on BTEX at different temperatures

[0046] The degradation experiment of Bacillus amyloliquefaciens byf-5 on BTEX was carried out at different temperatures, and it was found that it has a high ability to degrade benzene compounds at 25-30°C. From the perspective of practical application, 30°C is the most suitable temperature. At this time The removal rate is the highest, and the specific implementation steps are as follows:

[0047] In the inorganic medium containing benzene series compounds as the only carbon source, the final concentration of benzene series compounds is 50 mg / L of benzene, 50 mg / L of toluene, 50 mg / L of ethylbenzene, and 50 mg / L of o-xylene. Add freshly cultivated h1 -4 bacterial suspension, OD 600 0.2, the inoculum size is 1ml / 50ml, divide 50ml of the culture medium into five 250ml saline bottles, and...

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Abstract

The invention discloses a bacillus amyloliquefaciens capable of degrading benzene compounds, wherein the 16S rDNA of the bacillus amyloliquefaciens has a sequence of SEQ ID No.1, the strain is pale orange red, round, smooth and humid with neat edges in a solid medium, and the diameter thereof of the strain is between 1 and 2 millimeters; the form of the bacillus amyloliquefaciens is a thicker bacilli and is about 10 micrometers in length; spores are formed and are oval; and the bacillus amyloliquefaciens is aerobic, gram-positive and contact oxidase negative, can use a BTEX mixture or a single substance as a unique carbon source, and can degrade the BTEX mixture efficiently.

Description

technical field [0001] The invention relates to the technical field of biological treatment of benzene series compounds, in particular to a bacillus amyloliquefaciens capable of degrading benzene series compounds and an application thereof. Background technique [0002] Benzene compounds are a class of volatile monocyclic aromatic compounds, including benzene, toluene, ethylbenzene, xylene, etc., referred to as BTEX, widely used in petroleum, chemical, pesticide, textile, papermaking, paint, shoemaking and other industries It is a kind of toxic compound widely distributed in the environment. BTEX has "three effects", has been listed as a priority pollutant by many countries, and has been confirmed as a strong carcinogen. It is very important and urgent to develop BTEX pollution control technology in wastewater and waste gas. Currently, biodegradation technology is one of the most effective ways to degrade such substances. [0003] One of the keys to using biological metho...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/07C02F101/32
Inventor 陈建孟张丽丽王家德叶峰吴石金
Owner ZHEJIANG UNIV OF TECH
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