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Ion-exchange recovery method for synergistic matter in Bt fermentation

A substance, separation technology, applied in the fields of botanical equipment and methods, chemicals for biological control, biocides, etc., can solve the problems of virulence loss, high cost of sewage purification and treatment, and high COD content in the supernatant , to achieve the effect of strong anti-pollution ability, convenient and easy regeneration operation and good character

Active Publication Date: 2012-03-21
WUHAN KERNEL BIO-TECH CO LTD
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AI Technical Summary

Problems solved by technology

[0009] Due to the obvious synergistic effect of Zwittermicin A, centrifugation discards the supernatant, which leads to serious loss of virulence, and the supernatant has a high COD content, and the cost of sewage purification treatment is also high. The recovery process of Zwittermicin A has been studied

Method used

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Effect test

Embodiment 1

[0030] A method for the separation and recovery of synergistic substances in Bt fermentation, the steps are:

[0031] (1) The pretreatment of BT supernatant, carry out precipitation reaction under the condition of pH value 4 or 4.2 or 4.3 or 4.5 by adding protein flocculant zinc sulfate, potassium ferrocyanide and metal ion complexing agent oxalic acid, Remove the precipitate by plate and frame filtration, and adjust the pH of the filtrate to 7.0 before entering the column

[0032] About 20 tons (about 20 cubic meters) of the obtained whole supernatant of the normal Bt fermented liquid of each batch of fermentation is centrifuged by an industrial centrifuge, and a certain weight ratio of protein flocculant 0.15% zinc sulfate and potassium ferrocyanide 0.1% is added. Oxalic acid, then adjust the pH value to 4 or 4.1 or 4.2 or 4.4 or 4.5 and react for 2 or 3 or 4 hours. The reacted feed liquid enters the plate and frame filter for filtration, and the filtered clear liquid adjus...

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Abstract

The invention discloses an ion exchange recycling method for synergistic material in fermentation of Bt, comprising steps: (1) pre-treating Bt supernatant fluid, carrying out precipitation reaction by adding protein flocculating agent zinc sulfate, yellow potassium ferrocyanide and metallic ion complexing agent oxalic acid under condition of pH value, filtering and removing precipitate by a plateframe, and adjusting the pH value to pH6 before filtrate enters a column; (2) choosing polymeric adsorbent ZJ-16 with high adsorption capacity, good selectivity, high mechanical strength and long service life to adsorb synergistic material of Bt; and (3) collecting the obtained precipitated liquid and processing as follows according to needs: A, processing and producing Bt water suspension preparation with high valence, adding thinner assistants such as sodium chloride, sodium benzoate and xanthan gum into the precipitated liquid collected through ion exchange to produce Bt water aqua; B, processing Bt raw powder with high valence; and C, processing synergistic material powder for scientific research with high purity and high content. The method is simple and practical, improves the valence yield of a product, reduces production cost and improves the quality of the product.

Description

technical field [0001] The present invention relates to the technical field of recovered synergistic substances, and more specifically relates to a method for recovering synergistic substances in Bt fermentation. The synergistic substances in the supernatant recovered by the ion exchange process can be mixed with high-power centrifugal bacteria slurry to add The auxiliary agent can produce 16000IU / μl Bt SC liquid preparation, which can produce a new type of Bt preparation, enrich the variety of dosage forms, and meet the special market demand. The supernatant powder obtained by spray-drying the desorption liquid and the original powder can be mixed to produce high-quality Bt original powder, improve the potency recovery rate, and increase economic benefits. Background technique [0002] With the improvement of people's awareness of environmental protection, the ecological environment is getting worse and worse, people's requirements for pesticide residues are getting higher ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C237/22C07C231/24A01N37/46A01P7/00
Inventor 李青刘华梅陈振民程治国杨克华谢翔黄志远龚永华
Owner WUHAN KERNEL BIO-TECH CO LTD
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