Transgenic method for cultivated silkworm diapause variety

Abstract

The invention provides a method for making transgenic silkworms of a silkworm diapause variety. The method comprises the steps of utilizing the relationship between the environmental conditions of a parental generation and the diapause property of filial-generation silkworm eggs and utilizing a generation before transgenic operation to control the environmental conditions of the diapause silkworm variety so as to change the diapause property of a present generation of transgenic operation, performing silkworm-egg embryo microinjection on non-diapause silkworm eggs, utilizing fluorescent protein labeling to screen and making the transgenic silkworms. The method has the advantages of ingeniously breaking through the obstacles of transgenic technology for the silkworm diapause variety and effectively overcoming the defects of the prior transgenic method, and can lay a solid technical foundation for the large-scale application of silkworm transgenic technology in functional gene research, bioreactor development and other practical research.

Description

technical field [0001] The invention relates to the fields of biotechnology and bioengineering, in particular to a transgenic method for diapause varieties of silkworm. Background technique [0002] Transgenic technology is an important technology in modern biological research. It can effectively introduce artificially isolated or modified genes into the genome of organisms, and is the most powerful genetic tool for heterologous or ectopic expression. In the post-genome era, transgenic technology has irreplaceable advantages in functional gene research, biological material innovation, new product development, and modern genetic breeding. [0003] The silkworm is an important economic insect and a model organism of Lepidoptera. The silkworm genome frame map (2004) and fine map (2008) have been drawn successively, which marks that the research and understanding of silkworm has officially entered the post-genome era. Using efficient and stable technology to study the function...

AI Technical Summary

Problems solved by technology

Therefore, the use of polymorphic silkworm varieties as transgenic materials has obvious defects in the following two aspects: ① the economic traits of polymorphic silkworm varieties are poor, and the additional traits created by transgenes cannot reach the standard for direct promotion and application in terms of yield and quality , transgenic strains with polymorphic silkworm eggs as raw materials can only be used as raw materials for breeding, and as the original carrier of a certain related excellent trait, they can be used in the long and arduous traditional breeding

②Pluripotent silkworm varieties do not have a diapause period, and the maintenance of their subculture can only rely on continuous feeding and reproduction, which requires a lot of waste of manpower and material resources to maintain and subculture the obtained transgenic silkworm strains, and also greatly increases The risk of resource loss of obtained transgenic silkworm varieties

Although the above two patents have respectively provided corresponding transgenic methods for diapause species, the transgenic methods of most diapause species can also be realized accordingly, but the methods provided by them have their own limitations and shortcomings: the early pickling transgenic method of silkworm diapause species (Application No.: 200710303904.1) Due to the need for pickling, the hatching rate after microinjection is low, and the effect of early pickling is relatively large in differences among varieties; the low-temperature accelerated greening transgenic method of silkworm diapause species (Application No.: 200710303903.7) though After injection, the hatching rate is comparable to that of polymorphic silkworm eggs, but the effect of low-temperature acceleration of greening varies greatly among varieties, and it is basically not applicable to monomorphic and Japanese dimorphic silkworm varieties.

These shortcomings greatly limit the large-scale application of silkworm transgenic technology in practical research such as functional gene research and bioreactor development.