Halimodendron halodendron ERF transcription factor cDNA sequence, expression vector and use thereof

Abstract

The invention discloses a cDNA sequence of ERF transcription factor from Halimodendron halodendron, as well as an expression vector and application thereof, which belongs to the field of plant genetic engineering. A novel ERF2 ERF2 transcription factor gene HhERF2 (SEQ ID NO:1) is obtained by separating and cloning psammophyte Halimodendron halodendron through a RACE-PCR technique. The result of tissue-specific expression shows that the gene is expressed in roots, stalks and leaves of the Halimodendron halodendron. The result of stress response detection shows that the expression of the gene is induced by droughty, high-salt and low-temperature environmental stress and does not depend on the regulation of ABA. The cDNA sequence is transformed into Arabidopsis for expression; the stress resistance of transgenic Arabidopsis to drought, high salt, low temperature, diseases and the like is analyzed; and the relevant physiological and biochemical indexes of the transgenic Arabidopsis are determined. Experimental result shows that the cDNA sequence can effectively improve the drought resistance, osmotic-stress resistance and disease resistance of plants.

Description

technical field [0001] The present invention relates to a cDNA sequence related to plant stress resistance and the transcription factor encoded by it, in particular to the cDNA sequence encoding ERF transcription factor isolated and cloned from the psammophyte bell thorn (Halimodendron halodendron V.). The invention also relates to plant high-efficiency expression vectors containing the cDNA sequence and their application in improving plant stress resistance, belonging to the field of plant genetic engineering. Background technique [0002] The stress of plants includes abiotic stress (such as drought, salinity and low temperature, etc.) and biological stress (such as diseases, insect pests and weed damage caused by fungi, bacteria, viruses and nematodes). [0003] Drought, salinity and low temperature are three major abiotic stress factors that affect the growth of terrestrial plants and limit crop yield. According to statistics, the world's arid and semi-arid areas involv...

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Problems solved by technology

In 2008, the occurrence area of ​​major rice diseases and insect pests was 1.48 billion mu. Among them, the main diseases were sheath blight and rice blast, and the occurrence area was 400 million mu. The area is 1.08 billion mu, which is basically the same as that in 2007. In addition, important economic crops such as corn and cotton are seriously damaged by diseases and insect pests, causing huge economic losses

Although a large number of genes related to stress resistance have been cloned from various plants, most of these genes can only increase a single resistance of plants, and cannot comprehensively improve the stress resistance of plants as a whole.

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