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Improved prealbumin detection kit

A prealbumin and kit technology, applied in the field of medical immunology in vitro diagnosis, can solve the problems of inability to remove pseudo-turbidity, poor anti-interference ability, etc., so as to improve the anti-interference ability and sensitivity, save costs, and optimize determination. Effects of value methods

Inactive Publication Date: 2009-08-05
BEIJING STRONG BIOTECH INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The prealbumin detection reagents currently on the market cannot remove the influence of pseudo-turbidity very well, and the anti-interference ability is poor, so that the measured value of some special samples is zero or negative.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0073] Embodiment one: main component and preparation of kit I, II and III

[0074] The present application has exemplarily prepared 3 kinds of kits, and its main components and preparation steps are respectively:

[0075] 1. Main Components of Kits I, II and III

[0076] A. The main components of kit I:

[0077] Reagent R1:

[0078] Buffer (10mM Tris-HcL buffer containing 150mM NaCl, 0.1% EDTA, 0.1% NaN 3 , PH 6.5-8.0)

[0079] PEG6000 0.5%

[0080] Lauryl ether 0.02%

[0081] Tween20 0.02%

[0082] Reagent R2:

[0083] Buffer (10mM Tris-HcL buffer containing 150mM NaCl, 0.1% EDTA, 0.1% NaN 3 , PH 6.5-8.0)

[0084] Antiserum 10%

[0085] MgCl 2 2%

[0086] PEG6000 0.5%

[0087] Human serum matrix standard solution:

[0088] Mixed Human Health Serum

[0089] Penicillin 20000U

[0090] Nystatin 2mM

[0091] Lauryl ether 0.02%

[0092] B. Main Components of Kit II:

[0093] Reagent R1:

[0094] Buffer (50mM phosphate buffer containing ...

Embodiment 2

[0153] Embodiment 2: the main performance index of kit I, II, III

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Abstract

The invention discloses an improved immune transmission turbidity reagent kit for detecting the prealbumin content in blood serum, comprising a reagent R1, a reagent R2 and a prealbumin standard. Through the specific reaction of an antigen-antibody, the reagent kit forms a small immune compound which is smaller than 19s and forms a large visual compound which is larger than 19s under the action of a flocculant to generate a certain turbidity, thereby being suitable for measuring the transmission turbidity. The reagent can effectively prevent false turbidity from generating, has high accuracy rate, favorable reproducibility, strong capacity of resisting disturbance and simple operation and is suitable for various fully automatic biochemical analysers. The titer containing in the reagent adopts stroma with whole serum, thereby the influence of stroma effect is avoided.

Description

technical field [0001] The invention belongs to the field of medical immune in vitro diagnosis, and relates to an immunoturbidimetric detection reagent, in particular, the invention relates to a kit for detecting prealbumin in serum by immunoturbidimetric turbidimetry. Background technique [0002] Prealbumin is a serum protein synthesized by liver cells. It migrates before albumin during electrophoresis, so it is called prealbumin. Its main physiological function is to participate in the transport of thyroxine and retinol in serum, and it has thymus hormone activity. Increases body immunity by promoting the maturation of lymphocytes. Prealbumin is composed of four identical subunits, with a molecular weight of 54980, an extinction coefficient (E280nm) of 13.6, and a half-life of 1.9 days. [0003] The determination of prealbumin in serum is an excellent indicator of liver function and body nutritional status clinically, and it is common in malnourished patients. Because t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/539
Inventor 温云飞
Owner BEIJING STRONG BIOTECH INC
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