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Fast urine HIV detection diagnosis test paper and method for producing the same

A technology of test strips and urine, which is applied in the field of rapid urine HIV detection and diagnosis test strips and its preparation and use, to achieve the effects of convenient storage, wide application of people, and elimination of risk factors

Inactive Publication Date: 2009-07-22
关一夫
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved by the present invention is to overcome the defect that existing AIDS requires blood tests, and to provide a rapid urine HIV detection and diagnosis test strip and its preparation method and use method. The antibody status of HIV virus can be accurately determined within 5-15 minutes

Method used

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  • Fast urine HIV detection diagnosis test paper and method for producing the same
  • Fast urine HIV detection diagnosis test paper and method for producing the same
  • Fast urine HIV detection diagnosis test paper and method for producing the same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] like figure 1 As shown, the present invention is a rapid urine HIV detection diagnostic test strip, the test strip includes the base pad 1 at the bottom, the base pad is provided with a lateral flow chromatography pad 2, and the lateral flow chromatography pad is A detection line 21 and a quality control line 22 are provided, and the detection line is upstream of the quality control line; the upstream end of the lateral flow chromatography pad is provided with a gold-labeled binding pad 3 treated with the HIV antigen-colloidal gold complex, The upstream end of the gold standard binding pad is connected to the sample pad 4, and the downstream end of the lateral flow chromatography pad is connected to the absorbent pad 5; the end of the sample pad is the detection end of the test strip.

[0048] The lateral flow chromatography pad of the present invention is a nitrocellulose membrane.

[0049] The detection line of the present invention is coated with HIV antigen; the qu...

Embodiment 2

[0051] like figure 1 As shown, the present invention is a rapid urine HIV detection diagnostic test strip, the test strip includes the base pad 1 at the bottom, the base pad is provided with a lateral flow chromatography pad 2, and the lateral flow chromatography pad is A detection line 21 and a quality control line 22 are provided, and the detection line is upstream of the quality control line; the upstream end of the lateral flow chromatography pad is provided with a selenium-labeled binding pad 3 treated with HIV antigen-colloidal selenium complex, The upstream end of the selenium label binding pad is connected to the sample pad 4, and the downstream end of the lateral flow chromatography pad is connected to the water-absorbing pad 5; the end of the sample pad is the detection end of the test strip.

[0052] The lateral flow chromatography pad of the present invention is a nitrocellulose membrane.

[0053] The detection line of the present invention is coated with HIV anti...

Embodiment 3

[0062] Example 3 Preparation method

[0063] The preparation method comprises the following steps:

[0064] 1) Preparation of each component plate: including the preparation of the base pad, the preparation of the lateral flow chromatography pad, the preparation of the gold standard binding pad, the preparation of the sample pad and the preparation of the absorbent pad;

[0065] 2) Assemble each component board into a rapid urine HIV detection diagnostic test strip.

[0066] Specifically include the following steps:

[0067] 1. Preparation of colloidal gold

[0068] First, wash all the glassware used for colloidal gold with clean water to clean the dust on the glassware, add potassium dichromate, soak in concentrated sulfuric acid lotion for 24 hours, wash with high-purity water after silanization, and dry it for later use .

[0069] Take 100ml of chloroauric acid solution with a concentration of 0.01%, heat it to boiling, quickly add trisodium citrate with a concentration...

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Abstract

This invention discloses a urine test stripe for fast HIV diagnosis as well as a preparation method and a using method thereof. The test stripe consists of a base pad at the bottom and a lateral flow chromatographic pad, which is fixed at the former. Detection lines and quality control lines are arranged on the lateral flow chromatographic pad, and detection lines are positioned on upstream of quality control lines; the upstream end of the lateral flow chromatographic pad is connected to a binding pad treated by HIV antigen-colloidal gold compound or HIV antigen-colloidal selenium compound; the downstream end is connected to a blotting pad and the upstream end of the binding pad is connected to a sample pad. The test paper can correctly tell the HIV virus antibody condition of the person by testing the urine in 5-15 minutes. The invention has advantages of high testing precision, simple testing method, convenient sampling and high performance ratio, and it provides new and powerful technical support and product assurance for AIDS prevention and control in our country.

Description

technical field [0001] The invention relates to a rapid urine HIV detection and diagnosis test strip, a preparation method and a use method thereof. Background technique [0002] AIDS is an immunodeficiency disease caused by human immunodeficiency virus (HIV), which is extremely harmful to human health and life. In the past ten years, AIDS has spread rapidly in our country. As of December this year, the number of AIDS patients under the control of relevant departments in my country has reached 300,000, but due to personal privacy issues and other social reasons, the number of HIV-infected people in my country may be as high as 1 million. Since there is currently no effective preventive vaccine and treatment, early diagnosis is the best way to prevent HIV transmission. [0003] For the census and follow-up of AIDS-suspected groups and AIDS patients, the usual detection method is to conduct blood tests in designated hospital laboratories and disease inspection institutions. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N33/558G01N33/531
Inventor 关一夫王彪李晓娜赵晓阳郭亮郝风进
Owner 关一夫
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