Method for detecting trimethyl tin chloride and thorazine in human urine
A technology of trimethyl tin chloride and detection of human body, applied in the field of bioengineering, can solve the problems of neglecting the comprehensiveness and comprehensiveness of detection types, unfavorable industrialization to promote the general population, and the degree of contact with difficult pollutants, and achieves convenience and ease. The effect of obtaining, the method is convenient, and the detection cost is low
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[0032] Take 3 urine samples stored at -20°C, marked as No. 1, 2, and 3 respectively, centrifuge after natural thawing, and perform the following experimental procedures at the same time: draw 10ml of urine samples into the extraction bottle, add 5% NaOH200ul, 3g chlorine Add sodium chloride, then add 10ml ethyl acetate for extraction, vortex at 1500rpm for 5min, centrifuge at 3000rpm for 4min, suck out the organic phase, add 5g of anhydrous sodium sulfate to remove water, concentrate with nitrogen under normal temperature and pressure, and finally settle to 500ul after concentration Injection analysis. Take 1ul of the reaction solution for gas chromatography-mass spectrometry determination. The GC / MS conditions are: DB-5ms column, EI ion source, voltage of 70eV, ion source temperature of 230°C, and injection port temperature of 280°C. The experimental results were collected by SIM. The results are attached figure 1 , 2 , 3 shown. figure 1 It is the SIM chromatogram of uri...
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