Intellectualized bionic cultivation apparatus for tissue engineering tissue

Abstract

The invention discloses an intelligent biomimetic tissue cultivating device in tissue engineering, a gas-to-fluid and fluid-to-fluid exchanger is arranged between a tissue cultivating room and a fresh culture fluid storage bottle, the tissue cultivating room is connected with the gas-to-fluid and fluid-to-fluid exchanger through a circular pipeline, the fresh culture fluid storage bottle is communicated with the gas-to-fluid and fluid-to-fluid exchanger through another circular pipeline, a telescopic pump that is driven by a stepping motor is respectively arranged on each circular pipeline, the gas-to-fluid and fluid-to-fluid exchanger is communicated with O2 / CO2 / N2 sources through a gas transmitting pipe with a throttle, a forcing device that is driven by the stepping motor and simulates physiological stress of human beings is connected with a device used for exerting stress on a cultivated tissue of the tissue cultivating room, and a programmable logic controller is arranged for implementing intelligent control to the cultivation process. The intelligent biomimetic tissue cultivating device not only can provide the in vitro culture environment that is used for the in vitro tissue cultivation in tissue engineering and similar to the in vivo environment and truly realize biomimetic cultivation, but also can realize industrial production with intelligent control.

Description

technical field [0001] The invention relates to a biological tissue in vitro culture device in the field of biomedical engineering, in particular to an intelligent bionic culture device for tissue engineering tissue. Background technique [0002] Tissue engineering tissue cultivation in the field of biomedical engineering is to cultivate a biological tissue in vitro to construct a bioactive implant, and then implant the bioactive implant into the body to repair tissue defects and replace organ functions. Tissue engineering tissue cultivation requires that the cultured tissue be placed in the culture fluid in the culture chamber of the engineered tissue culture system for cultivation, and the culture fluid provides the required nutrition for the cultured tissue. The technical scheme of "Cyclic and Physiological Stress Simulation Engineered Tissue Three-Dimensional Culture Device" with the publication number CN1155690C provides a continuous perfusion to circulate the culture f...

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Problems solved by technology

This three-dimensional culture device for engineered tissue improves mass transfer to a large extent, promotes the growth and maturation of tissue engineering tissue, and improves the automation of tissue engineering tissue cultivation, but there are also the following problems: 1. Due to the new culture Both the liquid storage bottle and the circulating culture liquid storage bottle are equipped with exhaust ports, and a completely closed liquid flow channel is not formed. The end of the silicone tube in the circulation culture liquid storage bottle is provided with a microporous gas injector. The gas is provided to the circulating culture solution through the silicone tube inserted into the circulation culture solution storage bottle. 2 gas, making O 2 The gas generates bubbles in the circulating culture solution to supply oxygen. The bubbles will cause a large amount of foam on the liquid surface, which is easy to breed bacteria and cause pollution, resulting in tissue culture failure. Moreover, the circulation and physiological stress of this structure simulate the liquid used in the engineered tissue three-dimensional culture device. The flow driver can only use a peristaltic pump to drive the liquid flow, and its liquid flow form is continuous perfusion flow. This continuous perfusion flow is different from the pulse flow formed by the blood in the body under the state of systole and diastole, and is different from the real state of blood circulation in the body. Large difference; if the liquid flow driver uses a pulse telescopic pump to drive the culture liquid, it will cause the culture liquid to spray from the exhaust port

2. Since the middle part of the three-dimensional tissue cell culture chamber is only provided with a rack for resting the cultured tissue, the cultured tissue resting on the rack is soaked in the culture solution, and the culture solution exerts only static pressure and pressure when the culture solution flows on the cultured tissue. Shear force, can not apply compressive and tensile stress to the cultured tissue, which is quite different from the state of the tissue when the human body moves

3. The rack in the cultivation room is only suitable for the cultivation of massive tissues, but not for the cultivation of tubular or linear soft tissues

4. Since the new culture medium is added and mixed with the circulating culture medium, and a certain amount of new culture medium must be injected, a certain amount of circulating culture medium must be discharged, which will cause the amount of new culture medium to decrease until it is used In addition, the active factors secreted by the tissue will be partially lost along with the discharge of the culture medium and cannot be reused. The waste of the culture medium is large, and the long-term tissue culture process needs to be opened to add new culture medium, causing pollution. possibility, so the device cannot completely avoid the possibility of contamination

Due to the above shortcomings, the existing tissue engineering tissue culture devices do not have the real meaning of bionic cultivation, and it is difficult to meet the requirements of modern tissue engineering culture automation and industrialization development

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