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Method for preparing mixed type nucleotide feed addictive from yeast cell and application

A technology of feed additives and yeast cells, which is applied in the molding or processing of animal feed, application, animal feed, etc., can solve the problems of no occurrence, etc., and achieve the effects of reducing salt consumption, improving feed utilization rate, and increasing wall breaking rate

Inactive Publication Date: 2009-07-15
江苏省苏微微生物研究有限公司 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] So far, there are many domestic research papers on the use of nucleotides in animal feed, but there are no domestic patents that use nucleotides as animal feed additives and clearly propose the method of use and dosage.

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0051] 1. Stir the waste beer yeast with water evenly, pass through an 80-mesh sieve, settle and remove the supernatant, wash the sediment twice with water, and centrifuge (7000 rpm). The solids are waste yeast cells with high purity.

[0052] 2. Mix the pretreated waste beer yeast into water, use a high-pressure homogenizer to mechanically break the wall twice under a pressure of 35Mpa, then add solid salt, dissolve it, and prepare it to contain 10% yeast cells (dry weight) and 5% NaCl. yeast suspension.

[0053] 3. Heat the steam jacket in the reaction kettle to rapidly raise the temperature of the yeast emulsion to 95°C-100°C.

[0054] 4. After stirring and keeping warm for 4 hours under the condition of 95 ℃ ~ 100 ℃, the jacket is cooled to room temperature by cooling water.

[0055] 5. Centrifuge the above reaction solution (7000 r / min), the solid matter is denucleic acid yeast, mainly cell wall (rich in yeast glucan, mannan, etc.) and insoluble protein.

[0056] 6. Adj...

Embodiment 2

[0064] 1. Mix the mixed nucleotide additive containing 60.9% of monomeric nucleotides with the aquatic feed, the addition ratio is 0.03% (dry weight), and then through aging, tempering, granulation, and drying, it is prepared into a special Granular aquatic feed; used in the early growth stage of aquatic animals, or when aquatic animals are in a state of stress, and can also be used as an immune enhancer in the entire growth stage of aquatic animals. Feed 3 times a day, one in the morning, one in the middle and one in the evening. The feeding time for shrimp is 2 months; the feeding time for fish can be from 3 weeks to 17 weeks, generally 5 weeks to 8 weeks.

[0065] 2. Mix the mixed nucleotide additive containing 60.9% of monomeric nucleotides with livestock and poultry feed. For chicken feed and pig feed, the addition ratio is 0.02% to 0.08% (dry weight); for cattle feed, sheep feed For animal feed such as feed, the addition ratio is increased to 0.05% to 0.09% (dry weight)...

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Abstract

The invention relates to a method for preparing a mixed-type nucleotide feed additive from yeast cells and applications of the feed additive, which belong to the technical field of the preparation of nucleotide feed additives from yeast cells. The method comprises the steps: using yeast cells as raw materials and combining a homogenization method and a salt method to break yeast cell walls ; separating the yeast cell walls, protein and other insoluble substances by centrifugation and fractional isoelectric crystallization; specially collecting RNA precipitates at special isoelectric points to obtain wet and crude nucleic acid with the purity of 70 percent; dissolving the wet and crude nucleic acid in water, using high-activity phosphodiesterase from malt roots to carry out a high-efficiency directional enzymatic reaction; directly spraying, drying and crushing the solution after the enzymatic reaction to obtain the mixed-type nucleotide feed additive product comprising the protein content lower than 10 percent and four monosomic nucleotides (AMP, GMP, CMP and UMP) larger than 60 percent. After the feed additive is applied to animal feed, the intestinal absorption function of animals can be notably improved, the growth of the animals is stimulated, and the immune function, the antiviral capability and the anti-stress capability of the animals are improved.

Description

technical field [0001] The invention relates to a method for preparing a feed additive with a high content of monomeric nucleotides from yeast cells and applying it to animal feed, and belongs to the technical field of yeast preparation of nucleotides and feed additives. Background technique [0002] Yeast is a group of round or oval unicellular fungi whose main mode of reproduction is budding or division. It has a typical cell structure, including cell wall, cell membrane, cytoplasm, and nucleus. Yeast cells are a rich treasure trove of natural nutrition, with a protein content of up to 50% and a nucleic acid content of nearly 8%. In addition, it also contains a large amount of glucan, mannan and other immune polysaccharides and lipids, inorganic salts, vitamins, etc., which are very developed value. [0003] As a single-celled microorganism, yeast has the characteristics of fast reproduction, easy cultivation, and large output. For example, the annual output of beer in m...

Claims

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Application Information

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IPC IPC(8): A23K1/16A23K20/153A23K40/00
Inventor 张一平张南伟谢骏李靖叶进诸建国秦海萍
Owner 江苏省苏微微生物研究有限公司
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