Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Special culture medium for separating pathogen of soybean root rot

A technology for root rot bacteria and Phytophthora sojae, applied in fungi, microorganism-based methods, microorganisms, etc., can solve the problems of difficult separation and slow growth of Phytophthora sojae root rot bacteria, and achieves short experimental period and less labor. , good separation effect

Inactive Publication Date: 2009-07-08
NORTHEAST AGRICULTURAL UNIVERSITY +1
View PDF0 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

During the isolation process, Phytophthora sojae root rot grows slowly on common culture medium, while the associated main miscellaneous bacteria grow rapidly, often covering Phytophthora and inhibiting its growth, which makes the isolation of Phytophthora sojae root rot difficult

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Special culture medium for separating pathogen of soybean root rot
  • Special culture medium for separating pathogen of soybean root rot
  • Special culture medium for separating pathogen of soybean root rot

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1, the application of the selection medium I of the present invention in the isolation of Phytophthora soybean root rot

[0028] (1) The present invention selects the composition of culture medium I: Hymexazol (hymexazol) 0.5g, ceftriaxone sodium 0.2g, carrot 200.0g, agar powder 20.0g, H 2 O constant volume.

[0029] Preparation: Prepare the CA medium according to the above method, and sterilize it. When the CA medium is cooled to 65°C, add hymexazol and ceftriaxone sodium to the CA medium successively, shake well, and pour into the sterilized culture medium. in the dish.

[0030](2) PBNIC medium composition: Benlate (50% benomyl) 0.01g, Terrachlor (75% pentachloronitrobenzene) 0.054g, Rovral (50% iprodione) 0.04g, Neomycin (neomycin sulfate) 0.1g, Chloramphenicol (chloramphenicol) 0.01g, carrot 200.0g, agar powder 20.0g, H 2 O constant volume.

[0031] Preparation: After the sterilized CA medium is cooled to 65°C, add benomyl, pentachloronitrobenzene, ne...

Embodiment 2

[0059] Embodiment 2, separate with selection medium II of the present invention

[0060] Selection medium II of the present invention consists of: Hymexazol (hymexazol) 0.3g, ceftriaxone sodium 0.1g, carrot 200.0g, agar powder 20.0g, H 2 O constant volume.

[0061] Preparation: After the sterilized CA medium is cooled to 65°C, add hymexazol and ceftriaxone sodium successively, shake well, and pour it into a sterilized petri dish.

[0062] The experimental method is as described in Experiment 1, wherein the medium used is the selective medium II of the present invention, and the selective culture condition is: 5 days of dark culture in an incubator at 19°C.

[0063] The experiment was repeated 3 times, and the results showed that each diseased strain used in the experiment was isolated to obtain Phytophthora sojae root rot, and the isolation rate and the like were the same as the results in Example 1.

Embodiment 3

[0064] Embodiment 3, separate with selection medium III of the present invention

[0065] The composition of the selection medium III of the present invention: Hymexazol (hymexazol) 0.6g, ceftriaxone sodium 0.3g, carrot 200.0g, agar powder 20.0g, H 2 O constant volume.

[0066] Preparation: After the sterilized CA medium is cooled to 65°C, add hymexazol and ceftriaxone sodium successively, shake well, and pour it into a sterilized petri dish.

[0067] The experimental method is as described in Experiment 1, wherein the medium used is the selective medium III of the present invention, and the selective culture condition is: 7 days of dark culture in an incubator at 20°C.

[0068] The experiment was repeated 3 times, and the results showed that each diseased strain used in the experiment was isolated to obtain Phytophthora sojae root rot, and the isolation rate and the like were the same as the results in Example 1.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
lengthaaaaaaaaaa
Login to View More

Abstract

The invention discloses a special substrate for separating the soybean phytophthora root rot, which is a substrate obtained by adding 3-hydroxy-5-methyl isoxazolyl and [6R [6alpha, 7beta(Z)]]-3-[[(1,2,5,6-tetrahydrochysene-2- methyl-5,6-dioxo-1,2,4-triazine-3-yl) thio] methyl]-7-[[(2-amino-4-thiazolyl) (methoxyimino) acetyl] amino]-8-oxo-5-thia-1-azabicyclo [4.2.0] oct-2-ene-2-carboxylic acid disodium triple hemigydrate, which corespondingly have a final concentration of 0.3-0.6g / L and 0.1-0 .3 g / L in the special substrate for separating the soybean phytophthora root rot. The inventive substrate can effectively inhibit the growth of bacteria and mildew, and has good separation efficiency, simple composition and low cost. Accordingly, the present inventive substrate can be used for large-scale separation of soybean phytophthora root rot.

Description

technical field [0001] The invention relates to a special culture medium for isolating Phytophthora soybean root rot. Background technique [0002] Phytophthora root and stem rot of soybean (Phytophthora root and stem rot of soybean) is caused by Phytophthorasojae Kaufmann & Gerdemann infection. It is one of the most serious and devastating soybean diseases in the world. When the disease occurs, it can lead to the failure of soybean yield. At present, the more effective way to control soybean Phytophthora root rot is to breed varieties resistant to Phytophthora soybean root rot. And mastering the virulence type of Phytophthora soybean root rot is the basis and key work for disease-resistant breeding. Therefore, it is an important part of disease-resistant breeding to isolate and obtain Phytophthora soybean root rot to identify the virulence type. [0003] The isolation of Phytophthora soybean root rot has always been considered very difficult, because Phytophthora soybean...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12R1/845
Inventor 张淑珍吴俊江马凤鸣徐鹏飞李文滨刘丽君陈维元郭泰吕慧颖许修宏陈晨林蔚刚董德建钟鹏魏崃王志新鹿文成刘德生
Owner NORTHEAST AGRICULTURAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com