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Method for treating cultivation wastewater by using microbial flocculant and compound fertilizer obtained thereby

A microbial flocculant and aquaculture wastewater technology, applied in the direction of microbial-based methods, biological water/sewage treatment, biochemical equipment and methods, etc., can solve the problem of long time for sedimentation treatment, unsuitable for large-scale production, and long fermentation and culture time and other problems, to achieve the effect of short cycle time, shortened processing time and shortened production time

Inactive Publication Date: 2009-07-08
ZHEJIANG SCI-TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the method requires a long time for fermentation and cultivation, and the time for sedimentation treatment by microbial flocculation is also long, which not only leads to increased production costs, but is also not suitable for large-scale production.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Embodiment 1, a kind of method utilizing microbial flocculant to process aquaculture wastewater, carries out following steps successively:

[0021] 1), select Bacillus colloidus KNP414 as the production bacteria; the Bacillus colioids KNP414 can be purchased from the Institute of Bioengineering of Zhejiang Sci-tech University;

[0022] This strain was also reported in the "Ion Beam Implantation Technology Breeding Phosphorus Solubilizing Mutant Strain of Bacillus colloidus KNP414" published in the 4th issue of "Journal of Nuclear Agricultural Sciences" in 2008.

[0023] 2), strain activation and fermentation culture:

[0024] Formula (g / L) of selective medium, with deionized water as solvent:

[0025] Sucrose 5.0, Na 2 HPO 4 12H 2 O 0.6, MgSO 4 ·7H 2 O 0.2, soil minerals 1.0, CaCO 3 0.2, agar powder 15.0, pH7.0.

[0026] The formula (g / L) of seed bacteria production medium, with deionized water as solvent:

[0027] Sucrose 10.0, MgSO 4 ·7H 2 O 0.5,K 2 HPO ...

Embodiment 2

[0039] Embodiment 2, a kind of method utilizing microbial flocculant to process aquaculture wastewater, carries out following steps successively:

[0040] 1), select Bacillus colloidus (disclosed in the patent of patent No. 200410079363.5) with the preservation number of CGMCC 1231 as the production bacterium;

[0041] 2), strain activation and fermentation culture:

[0042] Selective medium formula (g / L), with deionized water as solvent:

[0043] Sucrose 6.0, Na 2 HPO 4 12H 2 O 1.0, MgSO 4 ·7H 2 O 0.2, soil minerals 1.0, CaCO 3 0.2, agar powder 15.0, pH7.3.

[0044] The formulation of the seed bacteria production medium is (g / L), with deionized water as the solvent:

[0045] Sucrose 10.0, MgSO 4 ·7H 2 O 0.2, K 2 HPO 4 ·3H 2 O 1.5, (NH 4 ) 2 SO 4 2.0, CaCO 3 0.2, pH7.3.

[0046] Liquid fermentation medium formula (g / L), with tap water as solvent:

[0047] Glucose 12.0, (NH 4 ) 2 SO 4 0.3, Na 2 HPO 4 12H 2 O 1.0, MgSO 4 ·7H 2 O 0.2, soil minerals 1....

Embodiment 3

[0056] Embodiment 3, a kind of method utilizing microbial flocculant to process aquaculture wastewater, carries out following steps successively:

[0057] 1), select Bacillus colioids with the preservation number CGMCC 1153 (patent No. 200410043837.0 discloses this bacterial strain) as the production bacterium;

[0058] 2), strain activation and fermentation culture:

[0059] Selective medium formula (g / L), with deionized water as solvent:

[0060] Sucrose 10.0, Na 2 HPO 4 12H 2 O 1.0, MgSO 4 ·7H 2 O 0.4, soil minerals 0.8, CaCO 3 0.4, agar powder 17.0, pH7.0.

[0061] The formulation of the seed bacteria production medium is (g / L), with deionized water as the solvent:

[0062] Sucrose 8.0, MgSO 4 ·7H 2 O 0.4, K 2 HPO 4 ·3H 2 O 1.0, (NH 4 ) 2 SO 4 2.5, CaCO 3 0.4, pH7.2.

[0063] Liquid fermentation medium formula (g / L), with tap water as solvent:

[0064] Sucrose 15.0, (NH 4 ) 2 SO 4 0.5, Na 2 HPO 4 12H 2 O 2.0, MgSO 4 ·7H 2 O 0.5, Soil Minerals...

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PUM

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Abstract

The invention discloses a method for treating culturing wastewater through utilization of a microorganism flocculant. The method comprises the following steps: 1) selection of bacillus mucilaginosus as a producing strain; 2) activation, fermentation and cultivation of a strain, wherein the strain is activated by a selective culture medium, inoculated in a culture medium for producing a seed strain and cultured to prepare the seed strain; and the seed strain is inoculated in a liquid fermentation culture medium and is fermented to obtain a fermentation liquid; 3) preparation of the microorganism flocculant, wherein the fermentation liquid is naturally precipitated; and a supernatant fluid is removed to prepare the microorganism flocculant; and 4) treatment on the culturing wastewater, wherein the microorganism flocculant and the culturing wastewater in volume ratio of 1 : 40-60 are mixed for 30 to 45 minutes and filtered; and filter residue is removed. The invention also provides a composite fertilizer prepared according to the method. The culturing wastewater treated by the method can reach emission standard and can also prepare composite fertilizer with good fertilizing effect.

Description

technical field [0001] The invention relates to a method for treating aquaculture wastewater by using a microbial flocculant, and a compound fertilizer produced by the method. Background technique [0002] Aquaculture wastewater belongs to the "three high" wastewater with high organic concentration, high N, P, K content and high number of harmful microorganisms, and it needs to be treated before it can be discharged into the environment. In wastewater treatment, flocculation is an indispensable process. The effect of traditional inorganic salt flocculants is not ideal, and aluminum salts are easy to cause Alzheimer’s disease and affect the ecological environment; organic polymer flocculants such as polyacrylamide have strong Neurotoxicity and strong teratogenic, carcinogenic and mutagenic effects. The unsafe and easy to cause secondary pollution of chemically synthesized flocculant greatly restricts its application. In contrast, microbial flocculants have the advantages of...

Claims

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Application Information

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IPC IPC(8): C02F1/52C02F3/34C05F7/00C12N1/20C12R1/085
CPCY02A40/20
Inventor 胡秀芳方琼楼余婕赵凯鹏陈集双
Owner ZHEJIANG SCI-TECH UNIV
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