Method for treating cultivation wastewater by using microbial flocculant and compound fertilizer obtained thereby
A microbial flocculant and aquaculture wastewater technology, applied in the direction of microbial-based methods, biological water/sewage treatment, biochemical equipment and methods, etc., can solve the problem of long time for sedimentation treatment, unsuitable for large-scale production, and long fermentation and culture time and other problems, to achieve the effect of short cycle time, shortened processing time and shortened production time
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Embodiment 1
[0020] Embodiment 1, a kind of method utilizing microbial flocculant to process aquaculture wastewater, carries out following steps successively:
[0021] 1), select Bacillus colloidus KNP414 as the production bacteria; the Bacillus colioids KNP414 can be purchased from the Institute of Bioengineering of Zhejiang Sci-tech University;
[0022] This strain was also reported in the "Ion Beam Implantation Technology Breeding Phosphorus Solubilizing Mutant Strain of Bacillus colloidus KNP414" published in the 4th issue of "Journal of Nuclear Agricultural Sciences" in 2008.
[0023] 2), strain activation and fermentation culture:
[0024] Formula (g / L) of selective medium, with deionized water as solvent:
[0025] Sucrose 5.0, Na 2 HPO 4 12H 2 O 0.6, MgSO 4 ·7H 2 O 0.2, soil minerals 1.0, CaCO 3 0.2, agar powder 15.0, pH7.0.
[0026] The formula (g / L) of seed bacteria production medium, with deionized water as solvent:
[0027] Sucrose 10.0, MgSO 4 ·7H 2 O 0.5,K 2 HPO ...
Embodiment 2
[0039] Embodiment 2, a kind of method utilizing microbial flocculant to process aquaculture wastewater, carries out following steps successively:
[0040] 1), select Bacillus colloidus (disclosed in the patent of patent No. 200410079363.5) with the preservation number of CGMCC 1231 as the production bacterium;
[0041] 2), strain activation and fermentation culture:
[0042] Selective medium formula (g / L), with deionized water as solvent:
[0043] Sucrose 6.0, Na 2 HPO 4 12H 2 O 1.0, MgSO 4 ·7H 2 O 0.2, soil minerals 1.0, CaCO 3 0.2, agar powder 15.0, pH7.3.
[0044] The formulation of the seed bacteria production medium is (g / L), with deionized water as the solvent:
[0045] Sucrose 10.0, MgSO 4 ·7H 2 O 0.2, K 2 HPO 4 ·3H 2 O 1.5, (NH 4 ) 2 SO 4 2.0, CaCO 3 0.2, pH7.3.
[0046] Liquid fermentation medium formula (g / L), with tap water as solvent:
[0047] Glucose 12.0, (NH 4 ) 2 SO 4 0.3, Na 2 HPO 4 12H 2 O 1.0, MgSO 4 ·7H 2 O 0.2, soil minerals 1....
Embodiment 3
[0056] Embodiment 3, a kind of method utilizing microbial flocculant to process aquaculture wastewater, carries out following steps successively:
[0057] 1), select Bacillus colioids with the preservation number CGMCC 1153 (patent No. 200410043837.0 discloses this bacterial strain) as the production bacterium;
[0058] 2), strain activation and fermentation culture:
[0059] Selective medium formula (g / L), with deionized water as solvent:
[0060] Sucrose 10.0, Na 2 HPO 4 12H 2 O 1.0, MgSO 4 ·7H 2 O 0.4, soil minerals 0.8, CaCO 3 0.4, agar powder 17.0, pH7.0.
[0061] The formulation of the seed bacteria production medium is (g / L), with deionized water as the solvent:
[0062] Sucrose 8.0, MgSO 4 ·7H 2 O 0.4, K 2 HPO 4 ·3H 2 O 1.0, (NH 4 ) 2 SO 4 2.5, CaCO 3 0.4, pH7.2.
[0063] Liquid fermentation medium formula (g / L), with tap water as solvent:
[0064] Sucrose 15.0, (NH 4 ) 2 SO 4 0.5, Na 2 HPO 4 12H 2 O 2.0, MgSO 4 ·7H 2 O 0.5, Soil Minerals...
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