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Method for producing biodegradable plastic from activated sludge

A technology for biodegrading plastics and activated sludge, applied in the field of environmental biology, can solve problems such as insufficient mass concentration, and achieve the effects of no need for sterile conditions, easy operation, and saving initial investment.

Inactive Publication Date: 2009-07-01
RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, in conventional activated sludge, the content of PHA in microbial cells in activated sludge is often less than 20% in mass concentration.

Method used

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  • Method for producing biodegradable plastic from activated sludge

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Effect test

Embodiment 1

[0022] The inventive method adopts technological process to see figure 1 . The activated sludge with polyhydroxyalkanoate storage capacity was cultivated by dynamic substrate dosing. First, 1 liter of activated sludge from a sewage treatment plant was inoculated in the sequencing batch reactor 3 (effective volume 2 liters), and then air was charged through the air pump 4 to start the aerobic reaction, which lasted 6 hours in total. In the first 10 minutes of the aerobic reaction, the mineral salt culture solution containing volatile fatty acids in the culture solution storage tank 1 is injected into the sequencing batch reactor 3 at a constant speed by the peristaltic pump 2, and the injection amount of the culture solution is 1 liter, and mixed The sludge concentration in the liquid is controlled at MLSS=2500~5000mg / L, and the concentration of the culture liquid is controlled at about 120Cmmol / L. 5 minutes before the end of the aerobic reaction, 83 ml of the mixture was dis...

Embodiment 2

[0026] The technological process of embodiment 2 is identical with embodiment 1, but concrete operation parameter is different, and difference is:

[0027] 1) The dosing time of the culture medium is increased from 10 minutes to 30 minutes;

[0028] 2) The aerobic reaction time of sequence batch reactor 3 is increased to 12 hours by 6 hours;

[0029] 3) The aerobic reaction time of reactor 5 is increased to 12 hours by 10 hours;

[0030] 4) The components and concentrations of the culture solution are different. The components of the culture solution used in Example 2 and the concentration of each component are: every liter of deionized water contains 30 cmmol of acetic acid, propionic acid, butyric acid and valeric acid, and 480 mg of ammonium sulfate, 200 mg of magnesium chloride, and 30 mg of valeric acid. EDTA, 240mg dipotassium hydrogen phosphate, 60mg potassium dihydrogen phosphate, 100mg calcium chloride, 40mg yeast extract and 2mL trace element solution. Trace elemen...

Embodiment 3

[0033] The technological process of embodiment 3 is identical with embodiment 1, but concrete operation parameter is different, and difference is:

[0034] 1) The dosing time of the culture medium is increased from 10 minutes to 20 minutes;

[0035] 2) The aerobic reaction time of sequence batch reactor 3 is reduced to 4 hours by 6 hours;

[0036]3) The aerobic reaction time of reactor 5 is reduced to 2 hours by 10 hours;

[0037] The culture solution used in embodiment 3 is the same as that in embodiment 1, but the sludge concentration in the mixed solution is controlled at MLSS=2500~5000mg / L, and the concentration of the culture solution is controlled at 60Cmmol / L. In embodiment 3, the productive rate of polyhydroxyalkanoate is 0.57Cmmol / Cmmol, polyhydroxyalkanoate weight accounts for 42% of microbial cell dry weight in microbial cell

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Abstract

The invention relates to the technical field of environmental biology, in particular to a method for producing biodegradable plastics (polyhydroxy paraffinic acid ester (PHA)) by using activated sludge. The method comprises the following steps: in a sequencing batch reactor, using the activated sludge as a strain, adding a mineral salt culture solution containing volatile fatty acid into the sequencing batch reactor by adopting a dynamic substrate feeding mode, and cultivating the activated sludge with storage power of the polyhydroxy paraffinic acid ester under oxic zone condition; transferring the activated sludge with the storage power of the polyhydroxy paraffinic acid ester discharged by the sequencing batch reactor to another reactor, and simultaneously feeding the mineral salt culture solution containing the volatile fatty acid into the another reactor, and making the polyhydroxy paraffinic acid ester stored in bacterial cells in the activated sludge saturated through oxic zone reaction; and extracting the polyhydroxy paraffinic acid ester by the sludge containing the polyhydroxy paraffinic acid ester entering the downstream technology. The method provides possibility for producing a novel environment-friendly material with low cost.

Description

technical field [0001] The invention relates to the field of environmental biotechnology, in particular to a method for producing polyhydroxyalkanoate (PHA) from activated sludge. Background technique [0002] Polyhydroxyalkanoate (PHA) is a kind of biopolyester, which can be synthesized by bacteria as an intracellular carbon source and energy store. PHA has mechanical properties similar to traditional plastics polyethylene and polypropylene, and is non-toxic, harmless, and completely biodegradable. It is a very good biodegradable plastic. Currently, the production of PHA is mainly through pure culture fermentation process. This process requires the screening of strains with high storage capacity, or the construction of genetically engineered bacteria through genetic recombination technology, and under pure culture conditions, aerobic fermentation to obtain biomass with higher intracellular PHA content, and then extract to obtain PHA. However, the PHA produced by this meth...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08G63/78C08G63/06
Inventor 刘俊新曲波
Owner RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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