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Method for breeding fermentation high stability hyaluronic acid sodium bacterial

A technique of sodium hyaluronate and a cultivation method, which is applied in the field of cultivation of fermented high-stability sodium hyaluronate strains, can solve problems such as poor stability of sodium hyaluronate and affect the effective use of sodium hyaluronate, and achieve high stability effect

Inactive Publication Date: 2009-06-10
TONGXIANG HENGJI BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0006] At present, the sodium hyaluronate produced by fermentation often has poor stability, which seriously affects the effective use of sodium hyaluronate. How to cultivate highly stable sodium hyaluronate is very necessary

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0043] The method for cultivating highly stable sodium hyaluronate strains provided in this example includes adopting numbering ATCC39920 as Streptococcus zooepidemicus, which is characterized in that the strains of Streptococcus zooepidemicus numbering ATCC39920 are cultured and fermented:

[0044] First: Streptococcus zooepidemicus producing sodium hyaluronate, optimized for long-term culture under the following conditions;

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PUM

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Abstract

The invention discloses a method for culturing a high-stability sodium hyaluronate strain, which comprises the step of adoption of streptococcus zooepidemicus with a number of ATCC39920. The method is characterized in that the streptococcus zooepidemicus with the number of ATCC39920 is subjected to strain culture and fermentation culture; after repeating the steps and performing repeated tests for 200 times, a strain for fermentation is obtained; fermentation culture of sodium hyaluronate is performed in a fermentation medium; and the fermentation medium is subjected to slight sterilization for 20 minutes at the temperature of 121 DEG C, cooled and cultured at a temperature of between 36 and 37 DEG C and at a pH value of between 6.8 and 7.1, enters the after-treatment step after culturing the sodium hyaluronate for 30 hours without limiting the air flow, and the high-stability sodium hyaluronate is obtained. Under the same conditions, the prior strain which is not subjected to optimization treatment is used for fermentation and production of the sodium hyaluronate; and the stability of the sodium hyaluronate finished product is obviously higher than that of an external sodium hyaluronate sample by comparing and examining the stability of the two types of sodium hyaluronate.

Description

technical field [0001] The invention relates to a cultivation and optimization technology of strains used in the fermentative production of sodium hyaluronate, in particular to a cultivation method for fermenting high-stability sodium hyaluronate strains. Background technique [0002] The importance of hyaluronic acid cannot be underestimated as it is present in all tissues of the body. One of the most important biological functions of hyaluronic acid is its water retention. Second, it was found that hyaluronic acid can provide nutrients to cells that do not have a direct blood supply, such as chondrocytes, and remove waste from these cells. When the content of hyaluronic acid is lower than the appropriate amount, nutrients cannot enter these cells and waste products cannot be excreted from these cells. Hyaluronic acid is abbreviated as HA. [0003] Scientific studies have proven that hyaluronic acid is found in synovial fluid, vitreous humor of the eye, cartilage, blood ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P19/04C12R1/46
Inventor 陈雪梅倪海良
Owner TONGXIANG HENGJI BIOTECH
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