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Improved antimicrobial peptides

An antimicrobial peptide and antimicrobial technology, applied in the field of antimicrobial peptides, can solve the problems of reducing effective use and increasing resistance problems, and achieve the effect of increasing possibility, enhancing effect and preventing infection

Inactive Publication Date: 2013-11-06
PERGAMUM AB
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is very likely that resistance problems will increase in the near future
In addition, several individuals have developed allergies to antibiotic agents, reducing the likelihood of effective use of certain antibiotic agents

Method used

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  • Improved antimicrobial peptides
  • Improved antimicrobial peptides
  • Improved antimicrobial peptides

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0097] Radial diffusion assay

[0098] It was generally performed as described earlier (Lehrer, RI, Rosenman, M., Harwig, SS, Jackson, R. & Eisenhauer, P. (1991) Ultrasensitive assays for endogenous antimicrobial polypeptides, J Immunol Methods. 137, 167-73.) Radial Diffusion Assay (RDA). The results are shown in Tables 1a-e.

[0099] In short, the bacteria (E. coli, Staphylococcus aureus) in 10 ml of full-strength (3% w / v) tryptic soy broth (TSB) (Becton-Dickinson, Cockeysville, MD) ) Or fungus (Candida albicans) cultured to midlogarithmic phase. Use 10mM Tris, pH7.4 to wash the microorganisms once. To 5ml of the underlay agarose gel (from 0.03% (w / v) TSB, 1% (w / v) low electroosmotic (Low-EEO) agarose (Sigma, St Louise MO) and 0.02% ( v / v) the final concentration of Tween20 (Sigma) composition) by adding 4x10 6 Bacterial cfu or 1x10 5 Fungal cfu. Pour the bottom layer into a petri dish with a diameter of 85 mm. After the agarose is solidified, a small hole with a diameter o...

Embodiment 2

[0127] Hemolyis assay

[0128] Centrifuge the EDTA-blood at 800 g for 10 minutes, and then remove the plasma and buffy coat. The red blood cells were washed 3 times, and then resuspended in 5% PBS, pH 7.4. The cells were then incubated at 37°C for 1 hour in the presence of peptide (3-60 µM) under conditions of end-over-end rotation. 2% Triton X-100 (Sigma-Aldrich) was used as a positive control. The sample was then centrifuged at 800g for 10 minutes. The absorbance of hemoglobin released was measured at λ540 nm, and the absorbance is expressed in the figure as the percentage of hemolysis induced by Triton-X100 (Table 1a).

[0129]

[0130]

[0131]

[0132]

[0133]

[0134] Table 1b

[0135]

[0136] Table 1c

[0137]

[0138] Table 1d

[0139]

[0140] Table 1e

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Abstract

The invention relates to an antimicrobial peptide comprising a first set of amino acid residues having a length of from about 2 to about 36 amino acid residues or analogues thereof linked to the amino or carboxyterminal end a second set comprising from 3 to 8 hydrophobic amino acid residue or analogue thereof, wherein said peptide has an antimicrobial activity.

Description

Invention field [0001] The present invention relates to an antimicrobial peptide comprising: a first group of amino acids having a length of about 2 to about 36 amino acid residues or their analogs linked to the amino or carboxy terminus, and comprising about 3 to 8 The second group of amino acid residues of hydrophobic amino acid residues or analogs thereof, wherein the peptide has antimicrobial activity. Background of the invention [0002] The immune system of mammals such as humans successfully fights several infections. However, in some cases, it is not always possible to eliminate bacteria, fungi or viruses, which can cause local or systemic acute infections. This is a serious concern in the perinatal care unit, burn care unit or intensive care unit and in immunocompromised individuals. Local acute infection causes extremely high morbidity. For example, Pseudomona saeruginosa (pseudomona saeruginosa) is the main cause of severe bacterial keratitis, and it is difficult to...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/47A61K38/04A61K38/10A61P31/04C07K7/08A61K38/39C07K14/78
Inventor A·施米特兴M·马尔姆斯滕
Owner PERGAMUM AB
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