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Rice protein OsOEE3-1, encoding gene and application thereof

A rice protein and coding technology, applied in the fields of application, genetic engineering, plant genetic improvement, etc., can solve the problems of unknown function of rice, unknown gene function, etc., and achieve the effect of increasing farmers' income, improving yield and quality, and increasing yield.

Inactive Publication Date: 2009-06-03
HANGZHOU ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0005] As we all know, the genetic map of rice has been drawn, that is, most of the rice gene sequences are public, but the specific function of a certain gene is unknown, especially the protein encoded by a certain gene and its effect on rice production. The functional impact of the

Method used

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  • Rice protein OsOEE3-1, encoding gene and application thereof
  • Rice protein OsOEE3-1, encoding gene and application thereof
  • Rice protein OsOEE3-1, encoding gene and application thereof

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Embodiment Construction

[0015] Gene acquisition

[0016] (1) The hybrid rice salt-tolerant combination Shanyou No. 10 and the salt-sensitive combination Liangyoupeijiu were used as materials, and their seeds were sown on filter paper culture dishes soaked in 100mM NaCl solution, placed in an incubator at 30°C to germinate, and the salt was changed every day. solution to keep the salt concentration essentially constant. After the seedlings grew to 10 days, the leaves of Shanyou 10 and Liangyoupeijiu seedlings were collected respectively.

[0017] (2) Precipitation with cold acetone / trichloroacetic acid (Salekdeh G H, Siopongco J, Wade L J, Ghareyazie B, Bennett J.A proteomic approach to analyzing drought-andsalt-responsiveness in rice. Field Crop Res, 2002, 76(2-3 ): 199-219) to quickly extract total leaf protein, the specific operation is as follows:

[0018] 1) Rice leaves are ground into fine powder with liquid nitrogen, put into 1.5ml centrifuge tubes, add 1ml protein extract solution I (acetone...

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Abstract

The invention provides and discloses a rice salt-resistant protein OsOEE3-1 with amino acid sequence described in a sequence table SEQ ID NO.1. The protein obtains over-expression in rice seedlings and can improve the high-salt-resistance ability and high-temperature-resistance ability of the rice seedlings. If a gene encoding the protein is used for converting Arabidopsis thaliana, pepper, eggplant, strawberry, Salvia splendens, gerbera hybrida and other vegetables and flowers, the high-salt-resistance and the high-temperature resistance performances of the plants are possibly improved, thereby being conductive to increasing the yield of rice or outdoor vegetables and flowers on saline-alkali soil, improving the utilization of the saline-alkali soil in coastal areas of China, overcoming the continuous cropping obstacle caused by soil salinization and high temperature under the facility conditions, improving the yield and the quality of vegetables, flowers and other plants, promoting the agricultural efficiency improvement and increasing the income of farmers.

Description

technical field [0001] The invention relates to the field of functional genomics, in particular to a rice protein OsOEE3-1 and its coding gene and application. Background technique [0002] The threat of soil salinization to agriculture is a global problem. There are a total of 1 billion hectares of saline-alkali land in the world, accounting for about 7.6% of the world's land area. my country's saline-alkali land is nearly 100 million hectares, and nearly 3.335 million hectares of agricultural land has been reduced or abandoned due to salinization. In recent years, my country's facility agriculture has developed rapidly, especially the production area of ​​vegetable and flower greenhouses has continued to expand. According to statistics, in 2005, the facility cultivation area of ​​vegetables, flowers, melons and fruits in China reached 2.1 million hectares. The development of facility agriculture has played an important role in the adjustment of agricultural production str...

Claims

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Application Information

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IPC IPC(8): C07K14/415C12N15/29A01H5/00
Inventor 阮松林马华升王世恒王淑珍童建新忻雅郑桂珍来文国白宇杰何俊平
Owner HANGZHOU ACAD OF AGRI SCI
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