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Anti-vancocin staphylococcus aureus culture medium

A vancomycin and staphylococcus technology, applied in biochemical equipment and methods, microbial determination/inspection, etc., can solve the problem of no vancomycin-resistant Staphylococcus aureus chromogenic medium, etc., and achieve operability. strong effect

Active Publication Date: 2009-05-20
AUTOBIO DIAGNOSTICS CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, although the chromogenic medium for methicillin-resistant S. aureus has appeared on the market, there is no chromogenic medium suitable for the identification of vancomycin-resistant S. aureus

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Each 1000g medium contains: agar 10g, tryptone 18g, yeast powder 7g, sodium pyruvate 11g, lithium chloride 8g, potassium tellurite 5mg, vancomycin 24mg, 5-bromo-4-chloro-3- Indole phosphate 1.5g, nitro blue tetrazolium 0.7g, the balance is water. After mixing the above raw materials evenly, the temperature is raised to 100-110°C, heated for 10 minutes, and then filled in a petri dish.

Embodiment 2

[0017] Each 1000g medium contains: agar 13g, tryptone 14g, yeast powder 2g, sodium pyruvate 8g, lithium chloride 2g, potassium tellurite 8mg, vancomycin 7mg, 5-bromo-4-chloro-3- Indole phosphate 1g, nitro blue tetrazolium 0.3g, the balance is water. After mixing the above raw materials evenly, the temperature is raised to 100-110°C, heated for 15 minutes, and then filled in a petri dish.

Embodiment 3

[0019] Each 1000g medium contains: agar 15g, tryptone 16g, yeast powder 4g, sodium pyruvate 5g, lithium chloride 6g, potassium tellurite 3mg, vancomycin 15mg, 5-bromo-4-chloro-3- Indole phosphate 1.8g, tetrazolium nitro blue 0.5g, the balance is water. After mixing the above raw materials evenly, the temperature is raised to 100-110°C, heated for 15 minutes, and then filled in a petri dish.

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Abstract

The invention belongs to the in vitro diagnostic reagent field, and particularly relates to a culture medium for identifying vancomycin-resistant Staphylococcus aureus. The culture medium not only contains nutrient substances, solidified material and miscellaneous bacteria inhibitor, but also contains vancomycin, as well as color indicator 5-bromo-4-chloro-3-indolyl-phosphate and nitroblue tetrazolium. The miscellaneous bacteria inhibitor can inhibit the growth of miscellaneous bacteria out of the Staphylococcus aureus, the vancomycin can inhibit the Staphylococcus aureus which is sensitive to the vancomycin, and the indicator can cause the vancomycin-resistant Staphylococcus aureus colony to show purple for being further differentiated from other drug-resistant bacteria. The invention combines the layers of screening culture of the vancomycin-resistant Staphylococcus aureus with the test of the characteristic enzyme thereof, can accurately and visually detect and identify the vancomycin-resistant Staphylococcus aureus, has strong operability, is suitable to be promoted in hospitals and other research institutions, and can help to guide the doctor for medicine use and develop novel antibiotics.

Description

technical field [0001] The invention belongs to the field of in vitro diagnostic reagents, in particular to a culture medium for vancomycin-resistant Staphylococcus aureus. Background technique [0002] In our country, the abuse of antibiotics is very common. After the bacteria have been in contact with the drug for many times, their sensitivity to the drug decreases or even disappears, resulting in the reduction or even ineffectiveness of the drug's efficacy against the drug-resistant bacteria. Staphylococcus aureus (referred to as Staphylococcus aureus) has successively defeated natural penicillin, ampicillin, cephalosporins, etc. in the "encounter" with antibiotics, and even staphylococcus aureus that can be resistant to vancomycin ( vancomycin-resistant staphylococcus aureus, abbreviated as VRSA). The continuous escalation of bacterial resistance has almost forced doctors to the embarrassing situation where there is no medicine available. The emergence of many drug-re...

Claims

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Application Information

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IPC IPC(8): C12Q1/14
Inventor 罗江卫王则宇康运凯冯长城崔晓晓杨红云郑业焕付光宇吴学纬
Owner AUTOBIO DIAGNOSTICS CO LTD
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