Method for simultaneously extracting lipid and protein from microalgae

Abstract

The invention relates to a method for simultaneously extracting grease and proteins from microalgae, which takes wet algae mud as a raw material, adjusts the pH value to be alkaline or slightly alkaline, and performs wall breaking of microalgae cells and dissolution of the grease and the proteins through vapor. The method is as follows: dissolved microalgae slurry is filtered for removal of cell residue, and a mixture of the grease and the proteins is obtained; a hydrocyclone is utilized for oil-water separation, and microalgae grease is obtained; and a protein aqueous solution is subjected to nano-filtration, condensation, isoelectric precipitation and freeze drying or spray drying, and protein powder is obtained. The method utilizes the water-bearing microalgae cells as the raw material, saves the energy consumption for drying the algae cells, adopts a slightly alkaline or alkaline algae cell liquid to realize vapor wall breaking and simultaneous dissolution of the grease and the proteins, and has good wall breaking effect, high dissolution rate, low energy consumption and easily widened process. The method adopts vortex hydrocyclone separation to well solve the problem of difficult oil-water separation under the condition of low phase ratio and realize simultaneous extraction of the microalgae grease and the proteins. Moreover, the method has the advantages of simple process, economy, low energy consumption, and easy realization of industrialization.

Description

technical field [0001] The invention belongs to the technical field of microalgae bioengineering, and in particular relates to a method for simultaneously extracting oil and protein from microalgae, especially for microalgae biodiesel refining and microalgae protein polygeneration. Background technique [0002] Microalgae are aquatic photoautotrophic microorganisms that use light energy to produce CO 2 and H 2 O acts as a substrate for cell growth, and synthesizes a large amount of proteins, triglycerides (TAGs), pigments, polysaccharides and other substances. Microalgae protein has important application value in food, medicine, feed and other fields, and triglycerides (TAGs), including unsaturated glycerides (DHA, EPA) can not only be used as important nutritional security products, but also can be used as It is an important source of oil raw material for biological liquid fuels (such as biodiesel). Especially with the worldwide shortage of fossil raw materials such as p...

AI Technical Summary

Problems solved by technology

However, when it is actually applied to microalgal cells such as chlorella with thicker cell walls and higher cell wall protein content, or diatom cells with siliceous shells such as Phaeodactylum tricornutum, the effect is not very good. , and the explosion when a large amount of high-pressure air is released instantaneously will cause the wet algae cells to splash, which is not conducive to cell collection

In addition, because oilseeds generally have high oil content and are dry seeds, when the aqueous method is used for oil extraction of oilseeds, the resulting slurry has a high oil content (that is, the oil-water ratio is large), so the traditional centrifugal separation method It is easier to achieve oil-water separation to obtain oil

For microalgae wet cells, the water content of the cells is as high as 80-95%, so the content of oil in the algae pulp after the wet cell water preparation method is broken and dissolved is very low (that is, the oil-water ratio is very low), and the upper phase after centrifugation is only A thin layer of oil phase, difficult to achieve oil phase collection

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