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Method for preparing inhibin genetic engineering regulation antigen

A technology of genetic engineering and inhibin, which is applied in the field of preparation of antigens regulated by inhibin genetic engineering, can solve the problems of low utilization rate of embryos and lack of excavation, and achieves the effects of good immunogenicity, improved utilization rate and simple preparation method.

Inactive Publication Date: 2009-05-06
SOUTH CHINA AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The conventional drug used is to inject FSH or FSH-like drugs into livestock to antagonize the effect of inhibin. According to the current results: the utilization rate of the embryos obtained during the superovulation process is very low, and the potential has not been explored.

Method used

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  • Method for preparing inhibin genetic engineering regulation antigen
  • Method for preparing inhibin genetic engineering regulation antigen
  • Method for preparing inhibin genetic engineering regulation antigen

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] In this example, the cDNA encoding porcine inhibin was cloned from the ovarian tissue of the Blue Tang pig to prepare the genetic engineering regulatory antigen of inhibin. It should be noted that the amino acid residue sequences of the mature inhibin peptides of different animals found so far are very similar, so in addition to using the porcine inhibin gene, the inhibin gene of other animals can also be used to prepare the inhibin gene Engineering Regulatory Antigens.

[0048] The preparation process specifically includes the following steps:

[0049] (1) Cloning the porcine inhibin coding gene;

[0050] (2) using the porcine inhibin coding gene to prepare a recombinant fusion protein of porcine inhibin;

[0051] (3) Purifying the expressed porcine inhibin recombinant fusion protein;

[0052] (4) Mixing the purified porcine inhibin recombinant fusion protein with mineral oil adjuvant to prepare porcine inhibin gene engineering regulation antigen.

[0053] The specif...

Embodiment 2

[0071] Embodiment 2 Test experiment

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Abstract

The invention discloses a method for preparing an inhibin genetic engineering regulation antigen, which comprises the following steps: cloning an inhibin encoding gene; making use of the inhibin encoding gene to prepare an inhibin recombinant fusion protein; and making use of the inhibin recombinant fusion protein to prepare the inhibin genetic engineering regulation antigen. The method for preparing the inhibin genetic engineering regulation antigen has the advantages of simplicity, high purity, good immunogenicity, low price and the like; the inhibin genetic engineering regulation antigen prepared by the method does not have toxicity, pathogenicity and drug residue, is harmless to human and animals, has no adverse effect on consumers, can improve the ovulation rate and the litter size, improve utilization ratio of a superovulation embryo, promote the sexual maturation so as to improve the reproduction and the like.

Description

technical field [0001] The invention belongs to the technical field of substances for regulating animal production traits, and in particular relates to a method for preparing an inhibin gene engineering regulating antigen. Background technique [0002] In the current process of intensive and industrialized development of animal husbandry, the production efficiency of animal husbandry has been greatly improved due to the application of genetic breeding technology, feed compounding technology, epidemic prevention technology, environmental sanitation technology and modern enterprise management technology. improvement. However, in each production link, the reproductive performance of animals has not improved in the past few decades. In domestic animals except pigs, most animals such as cattle and sheep only lay one egg and can only produce one litter during estrus. When producing a meat animal, a breeding female must be supported at the same time, causing the beef cattle indust...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/12C12N15/70C07K19/00C07K14/47A61K39/00A61P15/08
Inventor 施振旦黎敏义刘颖
Owner SOUTH CHINA AGRI UNIV
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