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Plant desensitizer and preparation method thereof

An anti-allergic agent, cactus technology, applied in the field of plant anti-allergic agent and its preparation, can solve the problems of high cost, difficult removal, cumbersome operation steps, etc., and achieve the effect of stable quality, simple extraction and separation method, and obvious anti-allergic effect

Active Publication Date: 2009-04-29
SHANGHAI PECHOIN BIOTECH CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the extraction of effective components of cactus is generally carried out through organic solvent extraction or mild alkali extraction. These methods require the introduction of organic solvents, which are costly and difficult to remove; moreover, isoelectric point precipitation is generally used in the protein separation process. The removal efficiency of miscellaneous proteins is low, and the removal process requires centrifugation, which is not conducive to industrial production; these extraction methods also have the disadvantages of cumbersome operation steps, many reagents are added, and the purity of the obtained products is not high.

Method used

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  • Plant desensitizer and preparation method thereof
  • Plant desensitizer and preparation method thereof
  • Plant desensitizer and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1. Production of plant anti-sensitizer

[0026] The production process of plant anti-sensitizers is as follows: figure 1 As shown, the specific process is as follows:

[0027] 1) Mix 100g of cactus powder (purchased from Xinsheng Biotechnology Development Co., Ltd.) and 2L of water at a mass ratio of 1:20, then add 20g of cellulase with an enzyme activity of 1 million U / g, and stir for 1 hour at 50°C , With no precipitation as an indicator, obtain an aqueous solution of cactus powder;

[0028] 2) Heat the above-mentioned aqueous solution of cactus powder at 80°C for 10 minutes to inactivate the enzyme. After the enzyme is inactivated, the solution is allowed to stand for 2 hours, and the supernatant is taken;

[0029] 3) Take 1800ml of the supernatant from the above step 2), adjust the pH to 4.2, and then add 36g of activated carbon to it so that the ratio of activated carbon to the supernatant is 1:50, and stir at 70°C for 1.5 hours;

[0030] 4) The reaction solut...

Embodiment 2

[0035] Example 2. Function test of plant anti-sensitizer

[0036] 1. Moisturizing effect experiment

[0037] Twenty volunteers were selected, half of the volunteers were male and female, the left and right forearms of the volunteers were washed with clean water respectively, and the moisturizing effect experiment was carried out without using any cosmetics.

[0038] Test the moisture content and TEWL value of the subject’s left and right forearm skin with a moisture tester, then apply the plant anti-sensitizer prepared in Example 1 on the subject’s left forearm, and use the right forearm as a blank control without applying any cosmetics . Test every half an hour for a total of 4 hours. The experiment is set for three repetitions. The average moisture content and the average moisture loss percentage of the skin surface of the subject change with time as shown in the figure figure 2 with image 3 Shown. The results show that the plant anti-sensitizer prepared in Example 1 has a goo...

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Abstract

The invention discloses a plant desensitizer and a preparation method thereof. The method comprises the following steps: 1) cellulose is used for treating cactus to degrade cell walls and obtain a cell lysate; 2) killing enzyme is carried out for the cell lysate obtained in Step 1, then the solution is allowed to stand and the upper clear liquid is taken; 3) the pH value of the upper clear liquid obtained in Step 2 is regulated to 4.2, then active carbon is added into the liquid, the mixture is stirred and filtered, filtrate is collected and a cactus extract is obtained; and 4) ion exchange chromatography is carried out to the cactus extract obtained in Step 3 to carry out desalination and decoloring, an effluent liquid is collected and the plant desensitizer is obtained. The preparation method of the plant desensitizer needs no organic reagent, has simple extraction and separation methods, and the obtained desensitizer has stable quality and significant anti-allergy effect.

Description

Technical field [0001] The invention relates to a plant anti-sensitizer and a preparation method thereof. Background technique [0002] Allergic reaction refers to the abnormal reaction of the human body to antigenic substances. The general manifestations of skin allergies are: dryness, itching, redness and hyperpigmentation. It is different from the normal immune response. Not only does it have no protective effect, but on the contrary, it causes physiological dysfunction and tissue damage due to over-reaction. Cactus contains a variety of phytonutrients, 18 kinds of amino acids, and various vitamins and minerals, which can bring precious nutrients and energy to the skin, can resist external irritation to the skin, and can effectively eliminate the skin caused by external irritation. Redness on the surface has anti-allergic and soothing effects. [0003] At present, the effective components of cactus are generally extracted by organic solvent extraction or mild alkaline extracti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/33A61P37/08A61K135/00
Inventor 董银卯何聪芬王昌涛王海涛
Owner SHANGHAI PECHOIN BIOTECH CO LTD
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