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Extractions and methods comprising elder species

A technology of extracts, species, applied in the field of extracts and methods including Elderberry species, capable of solving problems such as no contraindications

Inactive Publication Date: 2009-04-22
HERBALSCI SINGAPORE PTE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Additionally, Sambucus elderflower or elderberry extract compositions are generally considered safe and have no known contraindications

Method used

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  • Extractions and methods comprising elder species
  • Extractions and methods comprising elder species
  • Extractions and methods comprising elder species

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0357] Example of Step 1A: Single-step SFE maximum extraction and purification of elderberry

[0358] All SFE extractions were performed on SFT 250 (Supercritical Fluid Technologies, Inc., Newark, Delaware, USA) with a design pressure and temperature of up to 690 bar and 200°C, respectively. The device allows simple and effective extraction in dynamic or static mode operation flexibly under supercritical conditions. The device is mainly composed of three modules; oven, pump and controller, and collection module. The oven has a preheating column and a 100ml extraction vessel. The pump module has a compressed air driven pump with a constant flow rate of 300ml / min. The collection module is a 40ml glass bottle sealed with a cap and a septum for the recovery of the extracted product. The equipment is equipped with micro metering valves and flow meters. The pressure and temperature of the extraction vessel are monitored and controlled within ±3 bar and ±1°C.

[0359] In a typical embod...

Embodiment 2

[0361] Example of Step 2: Water Alcohol Extraction

Embodiment 3

[0364] Example of Step 3: Affinity adsorbent extraction of phenolic acid fraction (F1-F4 and F1-F6 fraction preparation)

[0365] In a typical experiment, the working solution is a transparent hydroalcoholic solution of the extract from the elderberry species in ethanol solution in step 2. The affinity adsorbent polymer resin is XAD7HP or ADS5. Before and after filling the column with ID 25mm and length 500mm, pre-wash 15gm ADS5 affinity adsorbent or 20gm XAD7HP affinity adsorbent with 95% ethanol (4-5BV) and distilled water (4-5BV) . The loaded solution is a crude 80% ethanol leaching phenolic acid solution, in which the chemical components are concentrated by rotary vacuum distillation and ethanol recovery. For XAD7HP loading, the final loading solution concentration was 29.03mg / ml, and for ADS5 loading, its concentration was 34.90mg / ml. At a flow rate of 0.3 BV / hr, 50 ml of the loading solution was loaded on the XAD7HP column, and 60 ml of the loading solution was loaded on t...

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Abstract

The present invention relates to extracts of elder species plant material prepared by supercritical CO2 extractions methods, methods of treating viruses in a subject and methods of inhibiting viral infections in cells thereof.

Description

[0001] Related application [0002] This application requires US provisional patent application No. 60 / 783,453 filed on March 17, 2006, 60 / 846,412 filed on September 22, 2006, and US provisional patent application No. 60 / filed on December 7, 2006 The priority of No. 873,473 is incorporated in this application by reference in its entirety. Invention field [0003] The present invention relates to extracts derived from elder sambucus species and methods thereof, as well as extracts extracted by such methods and methods of using such extracts, said extracts having substantially improved chemical composition of essential oils , Phenolic acid chemical composition, anthocyanidin or proanthocyanidin chemical composition, or phytohemagglutinin-polysaccharide chemical composition. Background of the invention [0004] The genus Sambucus, Sambuca nigra L., native to Europe, North America and West Asia, is a wild shrub. The genus Elderberry is further composed of more than 20 Sambuca species...

Claims

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Application Information

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IPC IPC(8): A01N65/00A61K36/00
CPCY02A50/30
Inventor R·T·高G·W·塞佩尔特D·李R·S·阿尔伯特
Owner HERBALSCI SINGAPORE PTE
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