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Synchronous separation, extraction and purification method for flavone, alkaloid and polysaccharide in lotus leaf

A simultaneous separation and lotus leaf flavonoid technology, applied in lotus leaf alkaloids and lotus leaf polysaccharides, extracting flavonoids, can solve the problems affecting the stability of flavonoids, high impurities in crude polysaccharides, high impurities in crude alkaloids, etc.

Active Publication Date: 2009-04-08
播恩集团股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This is mainly because when extracting a single component, the extraction conditions will destroy other components or the extraction rate of other components will be low, and the obtained extract will have high impurities, which will increase the difficulty and requirements of subsequent purification
For example, when flavonoids are extracted with water or organic solvents, some alkaloids can also be extracted, and the obtained crude flavonoids have high impurities, which increases the difficulty of subsequent purification and also affects the re-extraction of alkaloids
Similarly, when extracting alkaloids, the extraction conditions affect the stability of flavonoids, greatly affecting the re-extraction of flavonoids, and the obtained crude alkaloids have high impurities, which increases the difficulty of subsequent purification
When extracting polysaccharides with water, the pretreatment is complicated, and the extracted crude polysaccharides have high impurities, dark color, and are difficult to purify

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Take fresh lotus leaves, wash them with 0.1% NaCl-containing phosphoric acid solution (pH=3.0), dry them until the water content is 8%, crush them, pass through a 18-mesh sieve, weigh 100 g of the crushed lotus leaf dry powder, and microwave , the output power is 300W, the total treatment time is 2min, then add 2.5L of 50% ethanol solution adjusted by ammonia water to ph=13.0, add 0.02g of tertiary butyl hydroquinone (TBHQ) at the same time, heat, stir and extract for 2h , and filtered to obtain extract 1; add 2.5L of 90% ethanol solution adjusted to pH=2.0 by hydrochloric acid into the filter residue, add 0.05g of D-isoascorbic acid at the same time, heat, stir and extract for 2h, filter to obtain extract, and then add Add this extract to the residue and extract in the same way, filter to obtain the extract, combine the two extracts to obtain extract 2; add 3L of pure water to the residue, heat, stir and extract for 2 hours, and filter to obtain Extract 3.

[0022] Ta...

Embodiment 2

[0026] Take fresh lotus leaves, wash them with 0.1% NaCl-containing phosphoric acid solution (pH=4.0), dry them until the water content is 8%, pulverize them, pass through a 18-mesh sieve, weigh 100 g of pulverized lotus leaf dry powder, and microwave , the output power is 300W, the total treatment time is 2min, then add 2.5L of 50% ethanol solution adjusted by ammonia water to pH = 14.0, add 0.02g of dibutylhydroxytoluene (BHT) at the same time, heat, stir and extract for 2h, filter To obtain extract 1; add 2.5 L of 90% ethanol solution adjusted to pH = 3.0 with hydrochloric acid to the filter residue, add 0.05 g of D-isoascorbic acid at the same time, heat, stir and extract for 2 hours, filter to obtain the extract, and add to the residue Add this extract and extract in the same way, filter to obtain the extract, combine the two extracts to obtain extract 2; add 3L of pure water to the residue, heat, stir and extract for 2 hours, and filter to obtain extract Liquid 3.

[00...

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Abstract

The invention discloses a method for synchronously separating, extracting and purifying flavone, alkaloid and polyoses in lotus leaves. The method is characterized in that the lotus leaves are pretreated, added with different organic solvents and continuously leached for three times to obtain three kinds of leaching liquors of the flavone, the alkaloid and the polyoses respectively, and the three kinds of the leaching liquors are subjected to concentration, decoloration and drying treatment to obtain three kinds of products of the flavone, the alkaloid and the polyoses. The method overcomes the defects that the prior art can only extract a single component, destroys other components during the process of extracting the single component, has low extraction rate for the other components, high impurity content of extractives, and so on, gives attention to chemical properties of the flavone, the alkaloid and the polyoses, sufficiently considers mutual influence, activity and extraction rate of the extracting processes of various components and has the characteristics of high extraction rate, high purity and high utilization rate of lotus leaf resource.

Description

technical field [0001] The invention belongs to the field of deep processing of agricultural and forestry products, and relates to a method for extracting flavonoids, lotus leaf alkaloids and lotus leaf polysaccharides from lotus leaves. Background technique [0002] my country's lotus leaf resources are quite rich, and it has been listed in the second batch of "both food and medicine", which can be used as raw materials for the production of health food. For a long time, lotus leaf has been widely used in food and medicine. Lotus leaves are distributed in most parts of my country, mainly produced in Jiangxi, Hunan, Hubei, Zhejiang, Jiangsu and other places. Lotus leaf has the effects of relieving summer heat and clearing heat, promoting hair growth and clearing yang, dispelling blood stasis and stopping bleeding. It is also widely used clinically for obesity and hyperlipidemia, and has achieved good curative effect. The comprehensive utilization value is very high. [000...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/30C07H17/07C07G5/00C08B37/00A61K36/62
Inventor 邓泽元单斌范亚苇
Owner 播恩集团股份有限公司
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