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Method for producing valerian iridoids

The technology of a kind of iridoid and production method is applied in the production field of valerian iridoid, which can solve the problems of low purity of valerianin product and long period of total output period, so as to improve product purity and quality, Low production cost and stable active ingredients

Inactive Publication Date: 2009-03-25
CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above several methods are to concentrate and dry the extraction solvent, so the purity of the valerianin product obtained is not high, and the total output period is long

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0010] Embodiment one, this is a broad-leaf valerian (Valeriana officinalis L.var.latifoliaMiq) root is used as an explant to cultivate valerian iridoid cells to produce an example of iridoid products, and its production method is as follows Steps to proceed:

[0011] (1) Induction of valerian callus: prepare solid medium consisting of MS+2,4-D 2.5mg / L+KT1.8mg / L+6-BA 3.0mg / L+NAA 1.0mg / L, with acid Or alkali to adjust the pH value of the solid medium to 5.8, select valerian root as explants, inoculate on the above-mentioned prepared medium for cultivation, the cultivation temperature is 25°C±1°C, the light intensity is 1200lx, and the light time 18 hours a day, subculture once every 30 days on a solid medium, select fast-growing, light-colored uniform cells as subculture, and select valerian callus with high iridoid content.

[0012] (2) Cell suspension culture: The prepared liquid medium consists of MS+2,4-D 2.5mg / L+KT 1.8mg / L+6-BA 3.0mg / L+NAA 1.0mg / L+sucrose 40g / L+ethylene ...

Embodiment 2

[0014] Embodiment 2, this is one uses spider sweet (Valeriana jatamansi Jones) leaf as explant, cultivates valerian iridoid cell, produces the example of iridoid product, and its production method is carried out as follows:

[0015] (1) Valerian callus induction: the preparation of solid medium consists of MS+2, 4-D4mg / L+KT3.0mg / L+6-BA 5mg / L+NAA 3.0mg / L, with acid or alkali The pH value of the solid medium was adjusted to 7, and the leaves of the spider were selected as explants, and were inoculated on the prepared medium for cultivation. Subculture once every 15 days on the culture medium, select fast-growing, light-colored uniform granule cells as subculture, and select valerian callus with high iridoid content.

[0016] (2) Cell suspension culture: The prepared liquid medium consists of MS+2,4-D 4mg / L+KT 3.0mg / L+6-BA 5mg / L+NAA 3.0mg / L+sucrose 60g / L+ethephon 6mg / L, the pH was adjusted to 7, the above-mentioned callus cells were transferred to the liquid medium, and gradual...

Embodiment 3

[0018] Embodiment 3, select Valeriana officinalis L. (Valeriana officinalisL.) root to be the explant culture valerian iridoid cell, the example of producing iridoid product, its production method is carried out as follows:

[0019] (1) Valerian callus induction: the composition of the solid medium is MS+2, 4-D2.5mg / L+KT1.5mg / L+6-BA3.5mg / L+NAA 0.2mg / L, with acid Or alkali to adjust the pH to 6, select valerian root as explants, inoculate on the medium for cultivation, the cultivation temperature is 25°C±2°C, the light intensity is 1500lx, the light is 10 hours per day, and every 25 days on the solid medium Subculture once, select fast-growing, light-colored uniform cells as subculture, and select valerian callus with high iridoid content.

[0020] (2) Cell suspension culture: The prepared liquid medium consists of MS+2,4-D 2.5mg / L+KT 1.5mg / L+6-BA3.5mg / L+NAA 0.2mg / L+sucrose 35g / L+ethylene 3.5mg / L, pH adjusted to 6, above-mentioned callus cell is transferred in the liquid cultu...

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Abstract

The invention discloses a production method of valerian iridoid, which comprises the following steps: (1) induction cultivation and treatment of valerian callus: the explant of the valerian is inoculated to an MS solid medium containing corresponding plant growth regulatory substances for culture, successive transfer culture is carried out once every 15 days to 30 days, and the valerian callus containing more iridoid is selected; (2) cell suspension culture: the valerian callus is transferred to the liquid medium for gradual expansion culture and the valerian cells by expansion culture are inoculated to the liquid medium in a bioreactor for large-scale valerian cell suspension culture so as to obtain valerian cells; and (3) the iridoid is obtained by the extraction and separation of the valerian cells. The production method of the valerian iridoid resolves the problem that the production of valerian iridoid is short of raw materials, has high product purity, easy-control technical process and relatively low cost.

Description

technical field [0001] The invention relates to a production method of valerian iridoids. Background technique [0002] Valerian is a perennial herbaceous plant belonging to the genus Valeriana L. There are mainly four kinds of resources in our country: Valeriana officinalis L., Valeriana officinalis L.var. latifolia Miq., Valeriana amurensis Smir.ex Kom and spider incense ( Valeriana jatamansi Jones), from east to west, mainly distributed in south-central, southwest, northwest, northeast mountainous and hilly areas. Valerian root mainly contains iridoids, sesquiterpenes, alkaloids, lignans, flavonoids and other bioactive components, which have significant antispasmodic effects on smooth muscle, increase coronary flow, antiarrhythmia, anti Tumor, sedative and soothe the nerves, etc., is an excellent natural drug resource. Valeriana currently used in my country is basically in the wild state, artificial planting has just begun, and the iridoid content in wild valerian can ...

Claims

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Application Information

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IPC IPC(8): C12N5/04C12P17/06
Inventor 黎继烈李忠海方勤敏陈建华钟海雁彭映辉鲁海波王卫张慧
Owner CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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